A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal <i>Neisseria</i>—A Pilot Study
Commensal <i>Neisseria</i> provide a reservoir of resistance genes that can be transferred to the pathogens <i>Neisseria gonorrhoeae</i> and <i>N. meningitidis</i> in the human oropharynx. Surveillance programs are thus needed to monitor resistance in oropharyngea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/1/100 |
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| author | Jolein Gyonne Elise Laumen Saïd Abdellati Christophe Van Dijck Delphine Martiny Irith De Baetselier Sheeba Santhini Manoharan-Basil Dorien Van den Bossche Chris Kenyon |
| author_facet | Jolein Gyonne Elise Laumen Saïd Abdellati Christophe Van Dijck Delphine Martiny Irith De Baetselier Sheeba Santhini Manoharan-Basil Dorien Van den Bossche Chris Kenyon |
| author_sort | Jolein Gyonne Elise Laumen |
| collection | DOAJ |
| description | Commensal <i>Neisseria</i> provide a reservoir of resistance genes that can be transferred to the pathogens <i>Neisseria gonorrhoeae</i> and <i>N. meningitidis</i> in the human oropharynx. Surveillance programs are thus needed to monitor resistance in oropharyngeal commensal <i>Neisseria,</i> but currently the isolation and antimicrobial susceptibility testing of these commensals is laborious, complex and expensive. In addition, the posterior oropharyngeal/tonsillar swab, which is commonly used to sample oropharyngeal <i>Neisseria</i>, is poorly tolerated by many individuals. We evaluated an alternative non-invasive method to isolate oropharyngeal commensal <i>Neisseria</i> and to detect decreased susceptibility to azithromycin using selective media (LBVT.SNR) with and without azithromycin (2 µg/mL). In this pilot study, we compared paired posterior oropharyngeal/tonsillar swabs and oral rinse-and-gargle samples from 10 participants and demonstrated that a similar <i>Neisseria</i> species diversity and number of colonies were isolated from both sample types. Moreover, the proportion of <i>Neisseria</i> colonies that had a decreased susceptibility to azithromycin was similar in the rinse samples compared to the swabs. This pilot study has produced encouraging data that a simple protocol of oral rinse-and-gargle and culture on plates selective for commensal <i>Neisseria</i> with and without a target antimicrobial can be used as a surveillance tool to monitor antimicrobial susceptibility in commensal oropharyngeal <i>Neisseria.</i> Larger studies are required to validate these findings. |
| first_indexed | 2024-03-10T03:01:37Z |
| format | Article |
| id | doaj.art-43687488da5e44b1b17a5dc0702fe57f |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T03:01:37Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-43687488da5e44b1b17a5dc0702fe57f2023-11-23T12:45:16ZengMDPI AGAntibiotics2079-63822022-01-0111110010.3390/antibiotics11010100A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal <i>Neisseria</i>—A Pilot StudyJolein Gyonne Elise Laumen0Saïd Abdellati1Christophe Van Dijck2Delphine Martiny3Irith De Baetselier4Sheeba Santhini Manoharan-Basil5Dorien Van den Bossche6Chris Kenyon7STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumClinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumSTI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumDepartment of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), Université Libre de Bruxelles, 1000 Brussels, BelgiumClinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumSTI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumClinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumSTI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, BelgiumCommensal <i>Neisseria</i> provide a reservoir of resistance genes that can be transferred to the pathogens <i>Neisseria gonorrhoeae</i> and <i>N. meningitidis</i> in the human oropharynx. Surveillance programs are thus needed to monitor resistance in oropharyngeal commensal <i>Neisseria,</i> but currently the isolation and antimicrobial susceptibility testing of these commensals is laborious, complex and expensive. In addition, the posterior oropharyngeal/tonsillar swab, which is commonly used to sample oropharyngeal <i>Neisseria</i>, is poorly tolerated by many individuals. We evaluated an alternative non-invasive method to isolate oropharyngeal commensal <i>Neisseria</i> and to detect decreased susceptibility to azithromycin using selective media (LBVT.SNR) with and without azithromycin (2 µg/mL). In this pilot study, we compared paired posterior oropharyngeal/tonsillar swabs and oral rinse-and-gargle samples from 10 participants and demonstrated that a similar <i>Neisseria</i> species diversity and number of colonies were isolated from both sample types. Moreover, the proportion of <i>Neisseria</i> colonies that had a decreased susceptibility to azithromycin was similar in the rinse samples compared to the swabs. This pilot study has produced encouraging data that a simple protocol of oral rinse-and-gargle and culture on plates selective for commensal <i>Neisseria</i> with and without a target antimicrobial can be used as a surveillance tool to monitor antimicrobial susceptibility in commensal oropharyngeal <i>Neisseria.</i> Larger studies are required to validate these findings.https://www.mdpi.com/2079-6382/11/1/100<i>Neisseria</i> speciesantimicrobial susceptibilitysurveillance |
| spellingShingle | Jolein Gyonne Elise Laumen Saïd Abdellati Christophe Van Dijck Delphine Martiny Irith De Baetselier Sheeba Santhini Manoharan-Basil Dorien Van den Bossche Chris Kenyon A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal <i>Neisseria</i>—A Pilot Study Antibiotics <i>Neisseria</i> species antimicrobial susceptibility surveillance |
| title | A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal <i>Neisseria</i>—A Pilot Study |
| title_full | A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal <i>Neisseria</i>—A Pilot Study |
| title_fullStr | A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal <i>Neisseria</i>—A Pilot Study |
| title_full_unstemmed | A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal <i>Neisseria</i>—A Pilot Study |
| title_short | A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal <i>Neisseria</i>—A Pilot Study |
| title_sort | novel method to assess antimicrobial susceptibility in commensal oropharyngeal i neisseria i a pilot study |
| topic | <i>Neisseria</i> species antimicrobial susceptibility surveillance |
| url | https://www.mdpi.com/2079-6382/11/1/100 |
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