Meat quality - Genetic background and methods of its analysis

Growing consumer awareness is forcing food producers to supply raw material and products of increasingly high quality and health-promoting properties. Knowledge of the genetic background of quality characteristics is taking on great importance, enabling selection based on molecular markers. The incr...

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Main Authors: Marek Kowalczyk, Agnieszka Kaliniak-Dziura, Michał Prasow, Piotr Domaradzki, Anna Litwińczuk
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2022-01-01
Series:Czech Journal of Food Sciences
Subjects:
Online Access:https://cjfs.agriculturejournals.cz/artkey/cjf-202201-0002_meat-quality-genetic-background-and-methods-of-its-analysis.php
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author Marek Kowalczyk
Agnieszka Kaliniak-Dziura
Michał Prasow
Piotr Domaradzki
Anna Litwińczuk
author_facet Marek Kowalczyk
Agnieszka Kaliniak-Dziura
Michał Prasow
Piotr Domaradzki
Anna Litwińczuk
author_sort Marek Kowalczyk
collection DOAJ
description Growing consumer awareness is forcing food producers to supply raw material and products of increasingly high quality and health-promoting properties. Knowledge of the genetic background of quality characteristics is taking on great importance, enabling selection based on molecular markers. The increasing throughput of molecular techniques, in combination with an expanding bioinformatics infrastructure, is leading to continual improvement in understanding of the molecular mechanisms influencing meat quality. This has resulted in the identification of polymorphic nucleotides [single nucleotide polymorphisms (SNPs)] showing a relationship with meat characteristics such as tenderness [polymorphism in the calpain (CAPN) and calpastatin (CAST) genes], marbling [diacylglycerol o-acyltransferase 1 (DGAT1)], colour, pH and water-holding capacity (WHC) [CAST, stearoyl-CoA desaturase (SCD) and others], and fatty acid profile (SCD1). An increasingly wide range of methods is used for analysis, from techniques based on amplification of nucleic acids [polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system-PCR (ARMS-PCR)] through Sanger sequencing to high-throughput next-generation sequencing (NGS) techniques. This paper is a review of the literature on polymorphism of genes determining the quality characteristics of meat and molecular methods used to detect them.
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spelling doaj.art-43694143dfb74780bc36795c0b41609a2023-02-23T03:28:51ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172022-01-01401152510.17221/255/2020-CJFScjf-202201-0002Meat quality - Genetic background and methods of its analysisMarek Kowalczyk0Agnieszka Kaliniak-DziuraMichał Prasow1Piotr Domaradzki2Anna Litwińczuk3Institute of Quality Assessment and Processing of Animal Products, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences, Lublin, PolandInstitute of Quality Assessment and Processing of Animal Products, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences, Lublin, PolandInstitute of Quality Assessment and Processing of Animal Products, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences, Lublin, PolandInstitute of Quality Assessment and Processing of Animal Products, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences, Lublin, PolandGrowing consumer awareness is forcing food producers to supply raw material and products of increasingly high quality and health-promoting properties. Knowledge of the genetic background of quality characteristics is taking on great importance, enabling selection based on molecular markers. The increasing throughput of molecular techniques, in combination with an expanding bioinformatics infrastructure, is leading to continual improvement in understanding of the molecular mechanisms influencing meat quality. This has resulted in the identification of polymorphic nucleotides [single nucleotide polymorphisms (SNPs)] showing a relationship with meat characteristics such as tenderness [polymorphism in the calpain (CAPN) and calpastatin (CAST) genes], marbling [diacylglycerol o-acyltransferase 1 (DGAT1)], colour, pH and water-holding capacity (WHC) [CAST, stearoyl-CoA desaturase (SCD) and others], and fatty acid profile (SCD1). An increasingly wide range of methods is used for analysis, from techniques based on amplification of nucleic acids [polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system-PCR (ARMS-PCR)] through Sanger sequencing to high-throughput next-generation sequencing (NGS) techniques. This paper is a review of the literature on polymorphism of genes determining the quality characteristics of meat and molecular methods used to detect them.https://cjfs.agriculturejournals.cz/artkey/cjf-202201-0002_meat-quality-genetic-background-and-methods-of-its-analysis.phpmeat tendernessfatty acid profilemolecular markerssnpmolecular techniques
spellingShingle Marek Kowalczyk
Agnieszka Kaliniak-Dziura
Michał Prasow
Piotr Domaradzki
Anna Litwińczuk
Meat quality - Genetic background and methods of its analysis
Czech Journal of Food Sciences
meat tenderness
fatty acid profile
molecular markers
snp
molecular techniques
title Meat quality - Genetic background and methods of its analysis
title_full Meat quality - Genetic background and methods of its analysis
title_fullStr Meat quality - Genetic background and methods of its analysis
title_full_unstemmed Meat quality - Genetic background and methods of its analysis
title_short Meat quality - Genetic background and methods of its analysis
title_sort meat quality genetic background and methods of its analysis
topic meat tenderness
fatty acid profile
molecular markers
snp
molecular techniques
url https://cjfs.agriculturejournals.cz/artkey/cjf-202201-0002_meat-quality-genetic-background-and-methods-of-its-analysis.php
work_keys_str_mv AT marekkowalczyk meatqualitygeneticbackgroundandmethodsofitsanalysis
AT agnieszkakaliniakdziura meatqualitygeneticbackgroundandmethodsofitsanalysis
AT michałprasow meatqualitygeneticbackgroundandmethodsofitsanalysis
AT piotrdomaradzki meatqualitygeneticbackgroundandmethodsofitsanalysis
AT annalitwinczuk meatqualitygeneticbackgroundandmethodsofitsanalysis