Tn7382, a novel composite transposon harboring blaNDM-1 and aphA6 in Acinetobacter baumannii
Objectives: Co-transfer of carbapenem and amikacin resistance might contribute to the evolution of extensively drug resistant (XDR) Acinetobacter baumannii. The current study used bioinformatic tools and an in silico approach to investigate the potential mobility of a novel composite transposon co-h...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716522001916 |
| _version_ | 1798003416557944832 |
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| author | Samira M. Hamed Amira F.A. Hussein Mohamed H. Al-Agamy Hesham H. Radwan Mai M. Zafer |
| author_facet | Samira M. Hamed Amira F.A. Hussein Mohamed H. Al-Agamy Hesham H. Radwan Mai M. Zafer |
| author_sort | Samira M. Hamed |
| collection | DOAJ |
| description | Objectives: Co-transfer of carbapenem and amikacin resistance might contribute to the evolution of extensively drug resistant (XDR) Acinetobacter baumannii. The current study used bioinformatic tools and an in silico approach to investigate the potential mobility of a novel composite transposon co-harboring blaNDM-1 and aphA6. Methods: The transposon, named here Tn7382, was recently identified in the chromosomes of two XDR A. baumannii isolates (M02 and M11) from Egypt. The draft genomes of M02 and M11 were generated by Illumina sequencing. Nucleotide homology of Tn7382 and flanking regions was analyzed using the Basic Local Alignment Search Tool. Results: Tn7382 is derived from Tn125 and encompasses seven open reading frames [aphA6, ISAba125 transposase-coding gene, blaNDM-1, ble, iso, tat, cutA] enclosed by two direct copies of ISAba14. While described for the first time, Tn7382 was found in the chromosomes of five A. baumannii strains deposited in the NCBI database. Using the Artemis Comparison Tool, the potential mobility of Tn7382 was demonstrated in silico by comparative genomic analysis of two A. baumannii strains (TP1 and TP2) retrieved from the NCBI database. The transposon was acquired by TP2 at the same location as an ISAba14 element in the ancestral variant TP1 isolated from the same patient in the USA 11 days earlier. Conclusions: Here, we present the characteristics of Tn7382, a composite transposon flanked by ISAba14 and harboring the aphA6 and blaNDM-1 resistance genes. In silico analysis inferred the potential mobility of Tn7382, but experimental validation is still required. |
| first_indexed | 2024-04-11T12:07:15Z |
| format | Article |
| id | doaj.art-436c3f8138b9483fbe5d7a92debf1e78 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-04-11T12:07:15Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-436c3f8138b9483fbe5d7a92debf1e782022-12-22T04:24:42ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-09-0130414417Tn7382, a novel composite transposon harboring blaNDM-1 and aphA6 in Acinetobacter baumanniiSamira M. Hamed0Amira F.A. Hussein1Mohamed H. Al-Agamy2Hesham H. Radwan3Mai M. Zafer4Department of Microbiology and Immunology, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, EgyptDepartment of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, EgyptDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo, EgyptDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, Cairo, Egypt; Corresponding author. 4th Industrial Zone, Banks complex, 6th of October City, Cairo, Egypt. +201111112472.Objectives: Co-transfer of carbapenem and amikacin resistance might contribute to the evolution of extensively drug resistant (XDR) Acinetobacter baumannii. The current study used bioinformatic tools and an in silico approach to investigate the potential mobility of a novel composite transposon co-harboring blaNDM-1 and aphA6. Methods: The transposon, named here Tn7382, was recently identified in the chromosomes of two XDR A. baumannii isolates (M02 and M11) from Egypt. The draft genomes of M02 and M11 were generated by Illumina sequencing. Nucleotide homology of Tn7382 and flanking regions was analyzed using the Basic Local Alignment Search Tool. Results: Tn7382 is derived from Tn125 and encompasses seven open reading frames [aphA6, ISAba125 transposase-coding gene, blaNDM-1, ble, iso, tat, cutA] enclosed by two direct copies of ISAba14. While described for the first time, Tn7382 was found in the chromosomes of five A. baumannii strains deposited in the NCBI database. Using the Artemis Comparison Tool, the potential mobility of Tn7382 was demonstrated in silico by comparative genomic analysis of two A. baumannii strains (TP1 and TP2) retrieved from the NCBI database. The transposon was acquired by TP2 at the same location as an ISAba14 element in the ancestral variant TP1 isolated from the same patient in the USA 11 days earlier. Conclusions: Here, we present the characteristics of Tn7382, a composite transposon flanked by ISAba14 and harboring the aphA6 and blaNDM-1 resistance genes. In silico analysis inferred the potential mobility of Tn7382, but experimental validation is still required.http://www.sciencedirect.com/science/article/pii/S2213716522001916Acinetobacter baumanniiWhole genome sequencingWGSTransposonTn7382blaNDM-1 |
| spellingShingle | Samira M. Hamed Amira F.A. Hussein Mohamed H. Al-Agamy Hesham H. Radwan Mai M. Zafer Tn7382, a novel composite transposon harboring blaNDM-1 and aphA6 in Acinetobacter baumannii Journal of Global Antimicrobial Resistance Acinetobacter baumannii Whole genome sequencing WGS Transposon Tn7382 blaNDM-1 |
| title | Tn7382, a novel composite transposon harboring blaNDM-1 and aphA6 in Acinetobacter baumannii |
| title_full | Tn7382, a novel composite transposon harboring blaNDM-1 and aphA6 in Acinetobacter baumannii |
| title_fullStr | Tn7382, a novel composite transposon harboring blaNDM-1 and aphA6 in Acinetobacter baumannii |
| title_full_unstemmed | Tn7382, a novel composite transposon harboring blaNDM-1 and aphA6 in Acinetobacter baumannii |
| title_short | Tn7382, a novel composite transposon harboring blaNDM-1 and aphA6 in Acinetobacter baumannii |
| title_sort | tn7382 a novel composite transposon harboring blandm 1 and apha6 in acinetobacter baumannii |
| topic | Acinetobacter baumannii Whole genome sequencing WGS Transposon Tn7382 blaNDM-1 |
| url | http://www.sciencedirect.com/science/article/pii/S2213716522001916 |
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