CTLA-4 CT-60 A/G and CTLA-4 1822 C/T Gene Polymorphisms in Indonesians with Type 1 Diabetes Mellitus
Nur Rochmah,1 Muhammad Faizi,1 Suhasta Nova,1 Retno Asih Setyoningrum,1 Sukmawati Basuki,2 Anang Endaryanto1 1Faculty of Medicine, Department of Child Health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia; 2Institute of Tropical Disease, Universitas Airlangga, S...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-04-01
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| Series: | The Application of Clinical Genetics |
| Subjects: | |
| Online Access: | https://www.dovepress.com/ctla-4-ct-60-ag-and-ctla-4-1822-ct-gene-polymorphisms-in-indonesians-w-peer-reviewed-fulltext-article-TACG |
| _version_ | 1818011558429589504 |
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| author | Rochmah N Faizi M Nova S Setyoningrum RA Basuki S Endaryanto A |
| author_facet | Rochmah N Faizi M Nova S Setyoningrum RA Basuki S Endaryanto A |
| author_sort | Rochmah N |
| collection | DOAJ |
| description | Nur Rochmah,1 Muhammad Faizi,1 Suhasta Nova,1 Retno Asih Setyoningrum,1 Sukmawati Basuki,2 Anang Endaryanto1 1Faculty of Medicine, Department of Child Health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia; 2Institute of Tropical Disease, Universitas Airlangga, Surabaya, East Java, IndonesiaCorrespondence: Nur Rochmah, Faculty of Medicine, Department of Child Health, Dr. Soetomo General Hospital, Universitas Airlangga, Mayjend Prof. Dr. Moestopo No. 6-8, Surabaya, East Java, 60286, Indonesia, Tel +6281703501118, Email nur-r@fk.unair.ac.idIntroduction: CTLA-4 gene polymorphism plays an important role in children with type 1 diabetes mellitus (T1DM). However, data on this subject vary among different races and ethnics.Purpose: To analyze CTLA-4 CT-60 A/G and CTLA-4 1822 C/T gene polymorphism among children with T1DM compared to control.Patients and Methods: The CTLA-4 CT-60 A/G and CTLA-4 1822 C/T gene polymorphism in children with T1DM using polymerase chain reaction-restriction fragment length polymorphism in 25 T1DM and 25 controls. The inclusion criteria were patients regularly controlled at the Pediatric Endocrine Outpatient Clinic of Dr. Soetomo Hospital, aged 4– 18 years and willing to join this study and the exclusion criteria were T1DM patients hospitalized in the pediatric intensive care unit. In the control group, the inclusion criteria were healthy children, aged 4– 18 years and willing to join this study. The exclusion criteria included children with ongoing infection, history of other autoimmune diseases, allergies, or malignancy.Results: The mean age was 12.48 years old, and the mean of T1DM onset was 9.28 years old. The CTLA-4 1822 T allele observed in 62% T1DM and 56% in control (p = 0.388, OR = 0.78, 95% CI = 0.44– 1.37) and CTLA-4 CT-60 G allele observed in 52% T1DM and 58% in control (p = 0.393, OR = 1.27, 95% CI = 0.73– 2.22). The C/T genotypes was significantly higher in control group (p = 0.045, OR = 3.27, 95% CI = 1.00– 10.62). The A/G genotypes was commonly found in control group (p = 0.765, OR = 1.20, 95% CI = 0.37– 3.86). The Javanese was the dominant ethnic group in our study.Conclusion: The frequency of CTLA-4 CT-60 A/G polymorphism almost equivalent in T1DM and control group. However, CTLA-4 1822 C/T polymorphism was more prevalent in the control group; thus, this genotype may have a protective effect against T1DM.Keywords: type 1 diabetes mellitus, CTLA-4 |
| first_indexed | 2024-04-14T06:09:17Z |
| format | Article |
| id | doaj.art-436c8f291131462bb354039f8d12fadd |
| institution | Directory Open Access Journal |
| issn | 1178-704X |
| language | English |
| last_indexed | 2024-04-14T06:09:17Z |
| publishDate | 2022-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | The Application of Clinical Genetics |
| spelling | doaj.art-436c8f291131462bb354039f8d12fadd2022-12-22T02:08:24ZengDove Medical PressThe Application of Clinical Genetics1178-704X2022-04-01Volume 15192574899CTLA-4 CT-60 A/G and CTLA-4 1822 C/T Gene Polymorphisms in Indonesians with Type 1 Diabetes MellitusRochmah NFaizi MNova SSetyoningrum RABasuki SEndaryanto ANur Rochmah,1 Muhammad Faizi,1 Suhasta Nova,1 Retno Asih Setyoningrum,1 Sukmawati Basuki,2 Anang Endaryanto1 1Faculty of Medicine, Department of Child Health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia; 2Institute of Tropical Disease, Universitas Airlangga, Surabaya, East Java, IndonesiaCorrespondence: Nur Rochmah, Faculty of Medicine, Department of Child Health, Dr. Soetomo General Hospital, Universitas Airlangga, Mayjend Prof. Dr. Moestopo No. 6-8, Surabaya, East Java, 60286, Indonesia, Tel +6281703501118, Email nur-r@fk.unair.ac.idIntroduction: CTLA-4 gene polymorphism plays an important role in children with type 1 diabetes mellitus (T1DM). However, data on this subject vary among different races and ethnics.Purpose: To analyze CTLA-4 CT-60 A/G and CTLA-4 1822 C/T gene polymorphism among children with T1DM compared to control.Patients and Methods: The CTLA-4 CT-60 A/G and CTLA-4 1822 C/T gene polymorphism in children with T1DM using polymerase chain reaction-restriction fragment length polymorphism in 25 T1DM and 25 controls. The inclusion criteria were patients regularly controlled at the Pediatric Endocrine Outpatient Clinic of Dr. Soetomo Hospital, aged 4– 18 years and willing to join this study and the exclusion criteria were T1DM patients hospitalized in the pediatric intensive care unit. In the control group, the inclusion criteria were healthy children, aged 4– 18 years and willing to join this study. The exclusion criteria included children with ongoing infection, history of other autoimmune diseases, allergies, or malignancy.Results: The mean age was 12.48 years old, and the mean of T1DM onset was 9.28 years old. The CTLA-4 1822 T allele observed in 62% T1DM and 56% in control (p = 0.388, OR = 0.78, 95% CI = 0.44– 1.37) and CTLA-4 CT-60 G allele observed in 52% T1DM and 58% in control (p = 0.393, OR = 1.27, 95% CI = 0.73– 2.22). The C/T genotypes was significantly higher in control group (p = 0.045, OR = 3.27, 95% CI = 1.00– 10.62). The A/G genotypes was commonly found in control group (p = 0.765, OR = 1.20, 95% CI = 0.37– 3.86). The Javanese was the dominant ethnic group in our study.Conclusion: The frequency of CTLA-4 CT-60 A/G polymorphism almost equivalent in T1DM and control group. However, CTLA-4 1822 C/T polymorphism was more prevalent in the control group; thus, this genotype may have a protective effect against T1DM.Keywords: type 1 diabetes mellitus, CTLA-4https://www.dovepress.com/ctla-4-ct-60-ag-and-ctla-4-1822-ct-gene-polymorphisms-in-indonesians-w-peer-reviewed-fulltext-article-TACGtype 1 diabetes mellitusctla-4 |
| spellingShingle | Rochmah N Faizi M Nova S Setyoningrum RA Basuki S Endaryanto A CTLA-4 CT-60 A/G and CTLA-4 1822 C/T Gene Polymorphisms in Indonesians with Type 1 Diabetes Mellitus The Application of Clinical Genetics type 1 diabetes mellitus ctla-4 |
| title | CTLA-4 CT-60 A/G and CTLA-4 1822 C/T Gene Polymorphisms in Indonesians with Type 1 Diabetes Mellitus |
| title_full | CTLA-4 CT-60 A/G and CTLA-4 1822 C/T Gene Polymorphisms in Indonesians with Type 1 Diabetes Mellitus |
| title_fullStr | CTLA-4 CT-60 A/G and CTLA-4 1822 C/T Gene Polymorphisms in Indonesians with Type 1 Diabetes Mellitus |
| title_full_unstemmed | CTLA-4 CT-60 A/G and CTLA-4 1822 C/T Gene Polymorphisms in Indonesians with Type 1 Diabetes Mellitus |
| title_short | CTLA-4 CT-60 A/G and CTLA-4 1822 C/T Gene Polymorphisms in Indonesians with Type 1 Diabetes Mellitus |
| title_sort | ctla 4 ct 60 a g and ctla 4 1822 c t gene polymorphisms in indonesians with type 1 diabetes mellitus |
| topic | type 1 diabetes mellitus ctla-4 |
| url | https://www.dovepress.com/ctla-4-ct-60-ag-and-ctla-4-1822-ct-gene-polymorphisms-in-indonesians-w-peer-reviewed-fulltext-article-TACG |
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