Global characterization of OsPIP aquaporins reveals that the H2O2 transporter OsPIP2;6 increases resistance to rice blast
Plasma membrane intrinsic proteins (PIPs) are conserved plant aquaporins that transport small molecules across the plasma membrane to trigger instant stress responses and maintain cellular homeostasis under biotic and abiotic stress. To elucidate their roles in plant immunity to pathogen attack, we...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-02-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221451412300168X |
| _version_ | 1797289729032454144 |
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| author | Gousi Li Jingluan Han Chen Yi Hao Luo Yuzhu Wang Fengpin Wang Xiaoyu Wang Letian Chen Yaling Zhang |
| author_facet | Gousi Li Jingluan Han Chen Yi Hao Luo Yuzhu Wang Fengpin Wang Xiaoyu Wang Letian Chen Yaling Zhang |
| author_sort | Gousi Li |
| collection | DOAJ |
| description | Plasma membrane intrinsic proteins (PIPs) are conserved plant aquaporins that transport small molecules across the plasma membrane to trigger instant stress responses and maintain cellular homeostasis under biotic and abiotic stress. To elucidate their roles in plant immunity to pathogen attack, we characterized the expression patterns, subcellular localizations, and H2O2-transport ability of 11 OsPIPs in rice (Oryza sativa), and identified OsPIP2;6 as necessary for rice disease resistance. OsPIP2;6 resides on the plasma membrane and facilitates cytoplasmic import of the immune signaling molecule H2O2. Knockout of OsPIP2;6 increases rice susceptibility to Magnaporthe oryzae, indicating a positive function in plant immunity. OsPIP2;6 interacts with OsPIP2;2, which has been reported to increase rice resistance to pathogens via H2O2 transport. Our findings suggest that OsPIP2;6 cooperates with OsPIP2;2 as a defense signal transporter complex during plant–pathogen interaction. |
| first_indexed | 2024-03-07T19:09:11Z |
| format | Article |
| id | doaj.art-4371d29c53954b7394ba1194e0f948c1 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-03-07T19:09:11Z |
| publishDate | 2024-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-4371d29c53954b7394ba1194e0f948c12024-03-01T05:06:23ZengKeAi Communications Co., Ltd.Crop Journal2214-51412024-02-01121102109Global characterization of OsPIP aquaporins reveals that the H2O2 transporter OsPIP2;6 increases resistance to rice blastGousi Li0Jingluan Han1Chen Yi2Hao Luo3Yuzhu Wang4Fengpin Wang5Xiaoyu Wang6Letian Chen7Yaling Zhang8State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, Guangdong, China; Corresponding authors.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, Guangdong, China; National Key Laboratory of Plant Molecular Genetics, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China; Corresponding authors.Plasma membrane intrinsic proteins (PIPs) are conserved plant aquaporins that transport small molecules across the plasma membrane to trigger instant stress responses and maintain cellular homeostasis under biotic and abiotic stress. To elucidate their roles in plant immunity to pathogen attack, we characterized the expression patterns, subcellular localizations, and H2O2-transport ability of 11 OsPIPs in rice (Oryza sativa), and identified OsPIP2;6 as necessary for rice disease resistance. OsPIP2;6 resides on the plasma membrane and facilitates cytoplasmic import of the immune signaling molecule H2O2. Knockout of OsPIP2;6 increases rice susceptibility to Magnaporthe oryzae, indicating a positive function in plant immunity. OsPIP2;6 interacts with OsPIP2;2, which has been reported to increase rice resistance to pathogens via H2O2 transport. Our findings suggest that OsPIP2;6 cooperates with OsPIP2;2 as a defense signal transporter complex during plant–pathogen interaction.http://www.sciencedirect.com/science/article/pii/S221451412300168XAquaporinPlant immunityRice blastH2O2 transport |
| spellingShingle | Gousi Li Jingluan Han Chen Yi Hao Luo Yuzhu Wang Fengpin Wang Xiaoyu Wang Letian Chen Yaling Zhang Global characterization of OsPIP aquaporins reveals that the H2O2 transporter OsPIP2;6 increases resistance to rice blast Crop Journal Aquaporin Plant immunity Rice blast H2O2 transport |
| title | Global characterization of OsPIP aquaporins reveals that the H2O2 transporter OsPIP2;6 increases resistance to rice blast |
| title_full | Global characterization of OsPIP aquaporins reveals that the H2O2 transporter OsPIP2;6 increases resistance to rice blast |
| title_fullStr | Global characterization of OsPIP aquaporins reveals that the H2O2 transporter OsPIP2;6 increases resistance to rice blast |
| title_full_unstemmed | Global characterization of OsPIP aquaporins reveals that the H2O2 transporter OsPIP2;6 increases resistance to rice blast |
| title_short | Global characterization of OsPIP aquaporins reveals that the H2O2 transporter OsPIP2;6 increases resistance to rice blast |
| title_sort | global characterization of ospip aquaporins reveals that the h2o2 transporter ospip2 6 increases resistance to rice blast |
| topic | Aquaporin Plant immunity Rice blast H2O2 transport |
| url | http://www.sciencedirect.com/science/article/pii/S221451412300168X |
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