Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro
Herpes simplex virus type 1 (HSV-1) infections are one of the most common diseases in human population. HSV-1 causes subclinical, mild to severe diseases, especially in immunocompromised patients. Acyclovir has been used to reduce manifestations of HSV-1 infections. The extensive use of this drug ha...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Research in Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=2;spage=167;epage=174;aulast=Shabani |
| _version_ | 1819082544698621952 |
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| author | Mehdi Shabani Bahram Nasr Esfahani Bahar Sadegh Ehdaei Sharareh Moghim Arezoo Mirzaei Mohammadreza Sharifi Leili Mouhebat |
| author_facet | Mehdi Shabani Bahram Nasr Esfahani Bahar Sadegh Ehdaei Sharareh Moghim Arezoo Mirzaei Mohammadreza Sharifi Leili Mouhebat |
| author_sort | Mehdi Shabani |
| collection | DOAJ |
| description | Herpes simplex virus type 1 (HSV-1) infections are one of the most common diseases in human population. HSV-1 causes subclinical, mild to severe diseases, especially in immunocompromised patients. Acyclovir has been used to reduce manifestations of HSV-1 infections. The extensive use of this drug has led to the development of resistant strains. Thus, designing a novel anti-herpes drug with different mechanisms of action is urgently needed. Cellular microRNAs (miRNAs) have direct antiviral effects in addition to their regulatory functions. In this study we used a novel miRNA (hsa-miR-7704), expressed in macrophages, to inhibit HSV-1 lytic infection in HeLa cells. Synthesized hsa-miR-7704 mimics were transfected into HSV-1 infected HeLa cell. The inhibitory effects of the miRNA were evaluated by plaque assay, real time polymerase chain reaction and the viral titers were measured by the 50% tissue culture infective dose (TCID50). The viral titer and cell cytopathic effect were dramatically decreased in HeLa cells transfected with hsa-miR-7704 (50 and 100 nM), compared with HSV-1 infected cells alone or transfected with the mock miRNA control. These results suggest that hsa-miR-7704 inhibits HSV-1 replication efficiently in vitro. This may provide an alternative mechanism to prevent HSV-1 infections. |
| first_indexed | 2024-12-21T20:18:22Z |
| format | Article |
| id | doaj.art-437296da2b9e48f2be135b17075ae8cb |
| institution | Directory Open Access Journal |
| issn | 1735-5362 1735-9414 |
| language | English |
| last_indexed | 2024-12-21T20:18:22Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Research in Pharmaceutical Sciences |
| spelling | doaj.art-437296da2b9e48f2be135b17075ae8cb2022-12-21T18:51:33ZengWolters Kluwer Medknow PublicationsResearch in Pharmaceutical Sciences1735-53621735-94142019-01-0114216717410.4103/1735-5362.253364Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitroMehdi ShabaniBahram Nasr EsfahaniBahar Sadegh EhdaeiSharareh MoghimArezoo MirzaeiMohammadreza SharifiLeili MouhebatHerpes simplex virus type 1 (HSV-1) infections are one of the most common diseases in human population. HSV-1 causes subclinical, mild to severe diseases, especially in immunocompromised patients. Acyclovir has been used to reduce manifestations of HSV-1 infections. The extensive use of this drug has led to the development of resistant strains. Thus, designing a novel anti-herpes drug with different mechanisms of action is urgently needed. Cellular microRNAs (miRNAs) have direct antiviral effects in addition to their regulatory functions. In this study we used a novel miRNA (hsa-miR-7704), expressed in macrophages, to inhibit HSV-1 lytic infection in HeLa cells. Synthesized hsa-miR-7704 mimics were transfected into HSV-1 infected HeLa cell. The inhibitory effects of the miRNA were evaluated by plaque assay, real time polymerase chain reaction and the viral titers were measured by the 50% tissue culture infective dose (TCID50). The viral titer and cell cytopathic effect were dramatically decreased in HeLa cells transfected with hsa-miR-7704 (50 and 100 nM), compared with HSV-1 infected cells alone or transfected with the mock miRNA control. These results suggest that hsa-miR-7704 inhibits HSV-1 replication efficiently in vitro. This may provide an alternative mechanism to prevent HSV-1 infections.http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=2;spage=167;epage=174;aulast=Shabaniantiviral therapy; hsv-1; micro rna; mir-has-7704; mir-sx1. |
| spellingShingle | Mehdi Shabani Bahram Nasr Esfahani Bahar Sadegh Ehdaei Sharareh Moghim Arezoo Mirzaei Mohammadreza Sharifi Leili Mouhebat Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro Research in Pharmaceutical Sciences antiviral therapy; hsv-1; micro rna; mir-has-7704; mir-sx1. |
| title | Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro |
| title_full | Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro |
| title_fullStr | Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro |
| title_full_unstemmed | Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro |
| title_short | Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro |
| title_sort | inhibition of herpes simplex virus type 1 replication by novel hsa mir 7704 in vitro |
| topic | antiviral therapy; hsv-1; micro rna; mir-has-7704; mir-sx1. |
| url | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=2;spage=167;epage=174;aulast=Shabani |
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