Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress
Abstract Background The Polygonatum cyrtonema Hua rhizomes (also known as Rhizoma Polygonati, RP) are consumed for their health benefits. The main source of the RP is wild P. cyrtonema populations in the Hunan province of China. However, the soil Cadmium (Cd) content in Huanan is increasing, thus in...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-03-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04162-6 |
| _version_ | 1797865111221698560 |
|---|---|
| author | Rong Song Bei Yan Jin Xie Li Zhou Rui Xu Jia Min Zhou Xiong Hui Ji Zi Li Yi |
| author_facet | Rong Song Bei Yan Jin Xie Li Zhou Rui Xu Jia Min Zhou Xiong Hui Ji Zi Li Yi |
| author_sort | Rong Song |
| collection | DOAJ |
| description | Abstract Background The Polygonatum cyrtonema Hua rhizomes (also known as Rhizoma Polygonati, RP) are consumed for their health benefits. The main source of the RP is wild P. cyrtonema populations in the Hunan province of China. However, the soil Cadmium (Cd) content in Huanan is increasing, thus increasing the risks of Cd accumulation in RP which may end up in the human food chain. To understand the mechanism of Cd accumulation and resistance in P. cyrtonema, we subjected P. cyrtonema plants to four levels of Cd stress [(D2) 1, (D3) 2, (D4) 4, and (D5) 8 mg/kg)] compared to (D1) 0.5 mg/kg. Results The increase in soil Cd content up to 4 mg/kg resulted in a significant increase in tissue (root hair, rhizome, stem, and leaf) Cd content. The increase in Cd concentration variably affected the antioxidant enzyme activities. We could identify 14,171 and 12,115 protein groups and peptides, respectively. There were 193, 227, 260, and 163 differentially expressed proteins (DEPs) in D2, D3, D4, and D5, respectively, compared to D1. The number of downregulated DEPs increased with an increase in Cd content up to 4 mg/kg. These downregulated proteins belonged to sugar biosynthesis, amino acid biosynthesis-related pathways, and secondary metabolism-related pathways. Our results indicate that Cd stress increases ROS generation, against which, different ROS scavenging proteins are upregulated in P. cyrtonema. Moreover, Cd stress affected the expression of lipid transport and assembly, glycolysis/gluconeogenesis, sugar biosynthesis, and ATP generation. Conclusion These results suggest that an increase in soil Cd content may end up in Huangjing. Cadmium stress initiates expression changes in multiple pathways related to energy metabolism, sugar biosynthesis, and secondary metabolite biosynthesis. The proteins involved in these pathways are potential candidates for manipulation and development of Cd stress-tolerant genotypes. |
| first_indexed | 2024-04-09T23:03:57Z |
| format | Article |
| id | doaj.art-4372d28d0c2f45e4b2c7bc05f2f856c4 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-09T23:03:57Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-4372d28d0c2f45e4b2c7bc05f2f856c42023-03-22T10:50:03ZengBMCBMC Plant Biology1471-22292023-03-0123111710.1186/s12870-023-04162-6Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stressRong Song0Bei Yan1Jin Xie2Li Zhou3Rui Xu4Jia Min Zhou5Xiong Hui Ji6Zi Li Yi7College of Bioscience and Biotechnology, Hunan Agricultural UniversityCollege of Plant Protection, Hunan Agricultural UniversityInstitute of Agricultural Environment and Ecology, Hunan Academy of Agricultural SciencesInstitute of Agricultural Environment and Ecology, Hunan Academy of Agricultural SciencesInstitute of Agricultural Environment and Ecology, Hunan Academy of Agricultural SciencesInstitute of Agricultural Environment and Ecology, Hunan Academy of Agricultural SciencesInstitute of Agricultural Environment and Ecology, Hunan Academy of Agricultural SciencesCollege of Bioscience and Biotechnology, Hunan Agricultural UniversityAbstract Background The Polygonatum cyrtonema Hua rhizomes (also known as Rhizoma Polygonati, RP) are consumed for their health benefits. The main source of the RP is wild P. cyrtonema populations in the Hunan province of China. However, the soil Cadmium (Cd) content in Huanan is increasing, thus increasing the risks of Cd accumulation in RP which may end up in the human food chain. To understand the mechanism of Cd accumulation and resistance in P. cyrtonema, we subjected P. cyrtonema plants to four levels of Cd stress [(D2) 1, (D3) 2, (D4) 4, and (D5) 8 mg/kg)] compared to (D1) 0.5 mg/kg. Results The increase in soil Cd content up to 4 mg/kg resulted in a significant increase in tissue (root hair, rhizome, stem, and leaf) Cd content. The increase in Cd concentration variably affected the antioxidant enzyme activities. We could identify 14,171 and 12,115 protein groups and peptides, respectively. There were 193, 227, 260, and 163 differentially expressed proteins (DEPs) in D2, D3, D4, and D5, respectively, compared to D1. The number of downregulated DEPs increased with an increase in Cd content up to 4 mg/kg. These downregulated proteins belonged to sugar biosynthesis, amino acid biosynthesis-related pathways, and secondary metabolism-related pathways. Our results indicate that Cd stress increases ROS generation, against which, different ROS scavenging proteins are upregulated in P. cyrtonema. Moreover, Cd stress affected the expression of lipid transport and assembly, glycolysis/gluconeogenesis, sugar biosynthesis, and ATP generation. Conclusion These results suggest that an increase in soil Cd content may end up in Huangjing. Cadmium stress initiates expression changes in multiple pathways related to energy metabolism, sugar biosynthesis, and secondary metabolite biosynthesis. The proteins involved in these pathways are potential candidates for manipulation and development of Cd stress-tolerant genotypes.https://doi.org/10.1186/s12870-023-04162-6Cadmium stressDIA technologyHuangjingRhizome Cd contentSugar biosynthesisATP generation |
| spellingShingle | Rong Song Bei Yan Jin Xie Li Zhou Rui Xu Jia Min Zhou Xiong Hui Ji Zi Li Yi Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress BMC Plant Biology Cadmium stress DIA technology Huangjing Rhizome Cd content Sugar biosynthesis ATP generation |
| title | Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress |
| title_full | Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress |
| title_fullStr | Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress |
| title_full_unstemmed | Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress |
| title_short | Comparative proteome profiles of Polygonatum cyrtonema Hua rhizomes (Rhizoma Ploygonati) in response to different levels of cadmium stress |
| title_sort | comparative proteome profiles of polygonatum cyrtonema hua rhizomes rhizoma ploygonati in response to different levels of cadmium stress |
| topic | Cadmium stress DIA technology Huangjing Rhizome Cd content Sugar biosynthesis ATP generation |
| url | https://doi.org/10.1186/s12870-023-04162-6 |
| work_keys_str_mv | AT rongsong comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress AT beiyan comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress AT jinxie comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress AT lizhou comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress AT ruixu comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress AT jiaminzhou comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress AT xionghuiji comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress AT ziliyi comparativeproteomeprofilesofpolygonatumcyrtonemahuarhizomesrhizomaploygonatiinresponsetodifferentlevelsofcadmiumstress |