Correlation of TLR2 Arg677Trp and Arg753Gln polymorphisms with post-treatment serological test positive in syphilis patients
Objective: To investigate whether the Arg677Trp and Arg753Gln polymorphisms in the intracellular signaling region of TLR2 are linked to post-treatment serological test positive in syphilis patients. Methods: PCR-RFLP was used to detect TLR2 Arg677Trp and Arg753Gln polymorphisms in 100 syphilis patie...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology
2020-10-01
|
| Series: | Pifu-xingbing zhenliaoxue zazhi |
| Subjects: | |
| Online Access: | http://pfxbzlx.gdvdc.com/CN/abstract/abstract1863.shtml |
| _version_ | 1798029025514356736 |
|---|---|
| author | Dong-ping LU Rong ZHANG Fen WANG Jie JIA Biao FU Xiao-lan LU |
| author_facet | Dong-ping LU Rong ZHANG Fen WANG Jie JIA Biao FU Xiao-lan LU |
| author_sort | Dong-ping LU |
| collection | DOAJ |
| description | Objective: To investigate whether the Arg677Trp and Arg753Gln polymorphisms in the intracellular signaling region of TLR2 are linked to post-treatment serological test positive in syphilis patients. Methods: PCR-RFLP was used to detect TLR2 Arg677Trp and Arg753Gln polymorphisms in 100 syphilis patients with sero-fixation (group 1), 50 patients with TPPA positive while TRUST negative after syphilis treatment (group 2), and 100 healthy controls. DNA sequencing was used to confirm the diagnosis in case of diagnostic uncertainty. Results: TLR2 Arg677Trp polymorphism was not detected in any of the samples. While only one patient in group 1(1%) displayed TLR2 Arg753Gln polymorphism, proportion of patients with TLR2 Arg753Gln polymorphism was significantly higher in group 2 than in the controls (6/50 vs. 0, χ2=12.50, P<0.01). Conclusions: Neither TLR2 Arg677Trp nor Arg753Gln polymorphisms is associated to syphilis serum fixation. However, TLR2 Arg753Gln polymorphism could be linked to post-treatment TPPA positive and TRUST negative in syphilis patients. |
| first_indexed | 2024-04-11T19:18:31Z |
| format | Article |
| id | doaj.art-43733f3ce69c4afb8a012dee684c602b |
| institution | Directory Open Access Journal |
| issn | 1674-8468 |
| language | zho |
| last_indexed | 2024-04-11T19:18:31Z |
| publishDate | 2020-10-01 |
| publisher | editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology |
| record_format | Article |
| series | Pifu-xingbing zhenliaoxue zazhi |
| spelling | doaj.art-43733f3ce69c4afb8a012dee684c602b2022-12-22T04:07:22Zzhoeditoiral office of Journal of Diagnosis and Therapy on Dermato-venereologyPifu-xingbing zhenliaoxue zazhi1674-84682020-10-0127531231610.3969/j.issn.1674-8468.2020.05.004Correlation of TLR2 Arg677Trp and Arg753Gln polymorphisms with post-treatment serological test positive in syphilis patientsDong-ping LU0Rong ZHANG1Fen WANG2Jie JIA3Biao FU4Xiao-lan LU5Department of Dermatology, Shenzhen Bao’an Traditional Chinese Medicine Hospital Group, Shenzhen 518101, ChinaDepartment of Dermatology, Shenzhen Bao’an Traditional Chinese Medicine Hospital Group, Shenzhen 518101, ChinaDepartment of Obstetrics, Huangshi Maternity & Children’s Health Hospital E’dong Healthcare Group, Huangshi 435000, ChinaDepartment of Dermatology, Shenzhen Bao’an Traditional Chinese Medicine Hospital Group, Shenzhen 518101, ChinaE’dong Medical Care Group, Huangshi 435000, ChinaDepartment of Obstetrics, Huangshi Maternity & Children’s Health Hospital E’dong Healthcare Group, Huangshi 435000, ChinaObjective: To investigate whether the Arg677Trp and Arg753Gln polymorphisms in the intracellular signaling region of TLR2 are linked to post-treatment serological test positive in syphilis patients. Methods: PCR-RFLP was used to detect TLR2 Arg677Trp and Arg753Gln polymorphisms in 100 syphilis patients with sero-fixation (group 1), 50 patients with TPPA positive while TRUST negative after syphilis treatment (group 2), and 100 healthy controls. DNA sequencing was used to confirm the diagnosis in case of diagnostic uncertainty. Results: TLR2 Arg677Trp polymorphism was not detected in any of the samples. While only one patient in group 1(1%) displayed TLR2 Arg753Gln polymorphism, proportion of patients with TLR2 Arg753Gln polymorphism was significantly higher in group 2 than in the controls (6/50 vs. 0, χ2=12.50, P<0.01). Conclusions: Neither TLR2 Arg677Trp nor Arg753Gln polymorphisms is associated to syphilis serum fixation. However, TLR2 Arg753Gln polymorphism could be linked to post-treatment TPPA positive and TRUST negative in syphilis patients.http://pfxbzlx.gdvdc.com/CN/abstract/abstract1863.shtmltoll-like receptor 2genetic polymorphismsyphilisserological test |
| spellingShingle | Dong-ping LU Rong ZHANG Fen WANG Jie JIA Biao FU Xiao-lan LU Correlation of TLR2 Arg677Trp and Arg753Gln polymorphisms with post-treatment serological test positive in syphilis patients Pifu-xingbing zhenliaoxue zazhi toll-like receptor 2 genetic polymorphism syphilis serological test |
| title | Correlation of TLR2 Arg677Trp and Arg753Gln polymorphisms with post-treatment serological test positive in syphilis patients |
| title_full | Correlation of TLR2 Arg677Trp and Arg753Gln polymorphisms with post-treatment serological test positive in syphilis patients |
| title_fullStr | Correlation of TLR2 Arg677Trp and Arg753Gln polymorphisms with post-treatment serological test positive in syphilis patients |
| title_full_unstemmed | Correlation of TLR2 Arg677Trp and Arg753Gln polymorphisms with post-treatment serological test positive in syphilis patients |
| title_short | Correlation of TLR2 Arg677Trp and Arg753Gln polymorphisms with post-treatment serological test positive in syphilis patients |
| title_sort | correlation of tlr2 arg677trp and arg753gln polymorphisms with post treatment serological test positive in syphilis patients |
| topic | toll-like receptor 2 genetic polymorphism syphilis serological test |
| url | http://pfxbzlx.gdvdc.com/CN/abstract/abstract1863.shtml |
| work_keys_str_mv | AT dongpinglu correlationoftlr2arg677trpandarg753glnpolymorphismswithposttreatmentserologicaltestpositiveinsyphilispatients AT rongzhang correlationoftlr2arg677trpandarg753glnpolymorphismswithposttreatmentserologicaltestpositiveinsyphilispatients AT fenwang correlationoftlr2arg677trpandarg753glnpolymorphismswithposttreatmentserologicaltestpositiveinsyphilispatients AT jiejia correlationoftlr2arg677trpandarg753glnpolymorphismswithposttreatmentserologicaltestpositiveinsyphilispatients AT biaofu correlationoftlr2arg677trpandarg753glnpolymorphismswithposttreatmentserologicaltestpositiveinsyphilispatients AT xiaolanlu correlationoftlr2arg677trpandarg753glnpolymorphismswithposttreatmentserologicaltestpositiveinsyphilispatients |