Diversity of root-associated culturable fungi of Cephalanthera rubra (Orchidaceae) in relation to soil characteristics
Cephalanthera rubra (L.) Rich., Red Helleborine, is a widespread orchid in Europe but known only from three very small populations in England. These populations are in decline with no natural seed setting for more than a decade. The species may become extinct in the UK soon unless viable strategies...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2020-03-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/8695.pdf |
| _version_ | 1797419836282765312 |
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| author | Jake Bell Kazutomo Yokoya Jonathan P. Kendon Viswambharan Sarasan |
| author_facet | Jake Bell Kazutomo Yokoya Jonathan P. Kendon Viswambharan Sarasan |
| author_sort | Jake Bell |
| collection | DOAJ |
| description | Cephalanthera rubra (L.) Rich., Red Helleborine, is a widespread orchid in Europe but known only from three very small populations in England. These populations are in decline with no natural seed setting for more than a decade. The species may become extinct in the UK soon unless viable strategies are in place for ex situ conservation, especially the use of symbiotic propagation. Because of the fragile nature of the populations in England mycorrhizal fungal diversity study is not feasible. Therefore, to understand the factors needed for healthy Red Helleborine populations, soil characteristics and diversity of culturable root-derived fungi of the populations from a small area in the Loire Valley in France were studied. The main objectives of the study were: (1) Which culturable mycorrhizal fungi associated with C. rubra roots and (2) To what extent is variation in fungal communities related to variation in soil characteristics? Here, we report a significant difference in diversity of culturable mycorrhizal and non-mycorrhizal fungi depending on soil pH and phosphorus content. Mycorrhizal associations were favoured by plants in locations with low soil nutrient availability and comparatively higher pH. Our study shows that mycorrhizal fungi, both ecto and endo, can be cultured from roots of plants at different maturity stages. |
| first_indexed | 2024-03-09T06:53:00Z |
| format | Article |
| id | doaj.art-43775a9907754f73911844860d1a6a27 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:53:00Z |
| publishDate | 2020-03-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-43775a9907754f73911844860d1a6a272023-12-03T10:15:18ZengPeerJ Inc.PeerJ2167-83592020-03-018e869510.7717/peerj.8695Diversity of root-associated culturable fungi of Cephalanthera rubra (Orchidaceae) in relation to soil characteristicsJake Bell0Kazutomo Yokoya1Jonathan P. Kendon2Viswambharan Sarasan3Natural Capital and Plant Health, Royal Botanic Gardens, Kew, Richmond, Surrey, UKNatural Capital and Plant Health, Royal Botanic Gardens, Kew, Richmond, Surrey, UKComparative Plant and Fungal Biology, Royal Botanic Gardens, Kew, Richmond, Surrey, UKNatural Capital and Plant Health, Royal Botanic Gardens, Kew, Richmond, Surrey, UKCephalanthera rubra (L.) Rich., Red Helleborine, is a widespread orchid in Europe but known only from three very small populations in England. These populations are in decline with no natural seed setting for more than a decade. The species may become extinct in the UK soon unless viable strategies are in place for ex situ conservation, especially the use of symbiotic propagation. Because of the fragile nature of the populations in England mycorrhizal fungal diversity study is not feasible. Therefore, to understand the factors needed for healthy Red Helleborine populations, soil characteristics and diversity of culturable root-derived fungi of the populations from a small area in the Loire Valley in France were studied. The main objectives of the study were: (1) Which culturable mycorrhizal fungi associated with C. rubra roots and (2) To what extent is variation in fungal communities related to variation in soil characteristics? Here, we report a significant difference in diversity of culturable mycorrhizal and non-mycorrhizal fungi depending on soil pH and phosphorus content. Mycorrhizal associations were favoured by plants in locations with low soil nutrient availability and comparatively higher pH. Our study shows that mycorrhizal fungi, both ecto and endo, can be cultured from roots of plants at different maturity stages.https://peerj.com/articles/8695.pdfMycorrhizaCulture-dependentTripartitePlant-fungus interactionTree-orchid relationshipWoodland |
| spellingShingle | Jake Bell Kazutomo Yokoya Jonathan P. Kendon Viswambharan Sarasan Diversity of root-associated culturable fungi of Cephalanthera rubra (Orchidaceae) in relation to soil characteristics PeerJ Mycorrhiza Culture-dependent Tripartite Plant-fungus interaction Tree-orchid relationship Woodland |
| title | Diversity of root-associated culturable fungi of Cephalanthera rubra (Orchidaceae) in relation to soil characteristics |
| title_full | Diversity of root-associated culturable fungi of Cephalanthera rubra (Orchidaceae) in relation to soil characteristics |
| title_fullStr | Diversity of root-associated culturable fungi of Cephalanthera rubra (Orchidaceae) in relation to soil characteristics |
| title_full_unstemmed | Diversity of root-associated culturable fungi of Cephalanthera rubra (Orchidaceae) in relation to soil characteristics |
| title_short | Diversity of root-associated culturable fungi of Cephalanthera rubra (Orchidaceae) in relation to soil characteristics |
| title_sort | diversity of root associated culturable fungi of cephalanthera rubra orchidaceae in relation to soil characteristics |
| topic | Mycorrhiza Culture-dependent Tripartite Plant-fungus interaction Tree-orchid relationship Woodland |
| url | https://peerj.com/articles/8695.pdf |
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