Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinone
The mechanisms of disorders in cell functions induced by 1,4-naphthoquinone amide derivatives are not clarified yet. The article is dedicated to the study of features of these substances influence on loach Misgurnus fossilis L. embryos pro/antioxidant homeostasis during early embryogenesis. The aim...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2016-06-01
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| Series: | The Ukrainian Biochemical Journal |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2016/06/Bezkorovaynyj_3_16.pdf |
| _version_ | 1797427771191853056 |
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| author | A. O. Bezkorovaynyj A. R. Zyn N. M. Harasym J. T. Len O. M. Figurka D. I. Sanagursky |
| author_facet | A. O. Bezkorovaynyj A. R. Zyn N. M. Harasym J. T. Len O. M. Figurka D. I. Sanagursky |
| author_sort | A. O. Bezkorovaynyj |
| collection | DOAJ |
| description | The mechanisms of disorders in cell functions induced by 1,4-naphthoquinone amide derivatives are not clarified yet. The article is dedicated to the study of features of these substances influence on loach Misgurnus fossilis L. embryos pro/antioxidant homeostasis during early embryogenesis. The aim of this work was to study the effect of 2-chloro-3-hydroxy-1,4-naphthoquinone, 2-chloro-3-(3-oxo-3-(piperidine-1-yl)propylamine)-1,4-naphthoquinone (FO-1), 2-chloro-3-(3-(morpholine-4-yl)-3-oxopropylamine)-1,4-naphthoquinone (FO-2 at concentrations of 10-3, 10-5, 10-7 M on the content of TBA-reactive substances (a byproduct of lipid peroxidation) and the activities of superoxide dismutase and catalase in loach embryos. It was established that 1,4-naphthoquinone amide derivatives and 2-chloro-3-hydroxy-1,4-naphthoquinone decreased the content of lipid peroxidation products in embryo cells in a dose-dependent manner. The investigated compounds cause an increase in superoxide dismutase and catalase activities compared with the control value. The results of the two-factor ANOVA test indicate that 2-chloro-3-hydroxy-1,4-naphthoquinone and 1,4-naphthoquinone amide derivatives (FO-1, FO-2) have predominant influence on the TBA-reactive substances content and superoxide dismutase activity. However, the time of loach embryos development has a more pronounced effect on catalase activity than the studied 1,4-naphthoquinone derivatives. |
| first_indexed | 2024-03-09T08:49:51Z |
| format | Article |
| id | doaj.art-43777aabafdd4b4cb14fec09fd867990 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T08:49:51Z |
| publishDate | 2016-06-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-43777aabafdd4b4cb14fec09fd8679902023-12-02T14:35:55ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032016-06-01883465310.15407/ubj88.03.046Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinoneA. O. Bezkorovaynyj0A. R. Zyn1N. M. Harasym2J. T. Len3O. M. Figurka4D. I. Sanagursky5Ivan Franko National University of Lviv, Ukraine; Lviv Expert Center of Scientific Researches Ministry of Internal Affairs of UkraineLviv Expert Center of Scientific Researches Ministry of Internal Affairs of UkraineIvan Franko National University of Lviv, UkraineLviv Expert Center of Scientific Researches Ministry of Internal Affairs of Ukraine; National University “Lviv Polytechnicа”, UkraineNational University “Lviv Polytechnicа”, UkraineIvan Franko National University of Lviv, UkraineThe mechanisms of disorders in cell functions induced by 1,4-naphthoquinone amide derivatives are not clarified yet. The article is dedicated to the study of features of these substances influence on loach Misgurnus fossilis L. embryos pro/antioxidant homeostasis during early embryogenesis. The aim of this work was to study the effect of 2-chloro-3-hydroxy-1,4-naphthoquinone, 2-chloro-3-(3-oxo-3-(piperidine-1-yl)propylamine)-1,4-naphthoquinone (FO-1), 2-chloro-3-(3-(morpholine-4-yl)-3-oxopropylamine)-1,4-naphthoquinone (FO-2 at concentrations of 10-3, 10-5, 10-7 M on the content of TBA-reactive substances (a byproduct of lipid peroxidation) and the activities of superoxide dismutase and catalase in loach embryos. It was established that 1,4-naphthoquinone amide derivatives and 2-chloro-3-hydroxy-1,4-naphthoquinone decreased the content of lipid peroxidation products in embryo cells in a dose-dependent manner. The investigated compounds cause an increase in superoxide dismutase and catalase activities compared with the control value. The results of the two-factor ANOVA test indicate that 2-chloro-3-hydroxy-1,4-naphthoquinone and 1,4-naphthoquinone amide derivatives (FO-1, FO-2) have predominant influence on the TBA-reactive substances content and superoxide dismutase activity. However, the time of loach embryos development has a more pronounced effect on catalase activity than the studied 1,4-naphthoquinone derivatives.http://ukrbiochemjournal.org/wp-content/uploads/2016/06/Bezkorovaynyj_3_16.pdf |
| spellingShingle | A. O. Bezkorovaynyj A. R. Zyn N. M. Harasym J. T. Len O. M. Figurka D. I. Sanagursky Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinone The Ukrainian Biochemical Journal |
| title | Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinone |
| title_full | Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinone |
| title_fullStr | Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinone |
| title_full_unstemmed | Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinone |
| title_short | Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinone |
| title_sort | loach embryos prooxidant antioxidant status under the influence of amide derivatives of 1 4 naphthoquinone |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2016/06/Bezkorovaynyj_3_16.pdf |
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