Comparison of Capillary Zone Electrophoresis with High-pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies
Objective: The capillary zone electrophoresis (CZE) and highperformance liquid chromatography (HPLC) methods were compared in terms of HbA2 measurement for the assessment of hemoglobinopathies. Materials and Methods: CZE was compared with HPLC for the evaluation of patients without hemoglobinopathy...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2023-12-01
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| Series: | Turkish Journal of Hematology |
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| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-72593 |
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| author | Özlem Çakır Madenci Özlem Hürmeydan Asuman Orçun Fatma Erdoğmuş |
| author_facet | Özlem Çakır Madenci Özlem Hürmeydan Asuman Orçun Fatma Erdoğmuş |
| author_sort | Özlem Çakır Madenci |
| collection | DOAJ |
| description | Objective: The capillary zone electrophoresis (CZE) and highperformance liquid chromatography (HPLC) methods were compared in terms of HbA2 measurement for the assessment of hemoglobinopathies. Materials and Methods: CZE was compared with HPLC for the evaluation of patients without hemoglobinopathy (n=321), with β-thalassemia trait (n=113), and with common (HbD-Punjab, E, C, S/A, and S/S) and rare (HbS/D, O-Arap, Lepore, G-Coushata, Setif, Hamadan, Q-Iran, and H) variants (n=21). The reference range for HbA2 was determined by CZE. Results: Among patients without hemoglobinopathy, the median (2.5th-97.5th percentiles) values were 97.4% (97.0-98.0%) and 97.5% (96.6-98.4%) for HbA (p=0.060) and 2.4% (1.6-3.0%) and 2.5% (1.6-3.1%) for HbA2 (p<0.001) by HPLC and CZE, respectively. The reference range for HbA2 was 1.6-3.1% by CZE. In the comparison of methods for HbA2, there was a constant error of 0.255 (confidence interval: 0.062-0.448) and bias of 0.10% (limit of agreement: 0.33- 0.53), and higher values were obtained with CZE. A strong correlation was observed between the methods (r=0.782). Interrater agreement was almost perfect for clinical diagnosis (κ=0.911). The two methods detected and identified the common variants similarly. All rare variants, except HbH by HPLC and HbS/D by CZE, were detected as separate peaks by both methods. Conclusion: The two methods were in agreement regarding the preliminary identification of β-thalassemia patients. Different Hb variants were detected by both methods but with possible methodological interference for HbA2 measurements. CZE is a reliable and simple alternative for the evaluation of hemoglobinopathies. The standardization of HbA2 measurements should be prioritized as more techniques become available in routine laboratory practice. |
| first_indexed | 2024-03-09T02:40:13Z |
| format | Article |
| id | doaj.art-4377a0da1b6947eba902f32a2e3c2dc5 |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-03-09T02:40:13Z |
| publishDate | 2023-12-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-4377a0da1b6947eba902f32a2e3c2dc52023-12-06T06:27:23ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632023-12-0140425826510.4274/tjh.galenos.2023.2023.0114TJH-72593Comparison of Capillary Zone Electrophoresis with High-pressure Liquid Chromatography in the Evaluation of HemoglobinopathiesÖzlem Çakır Madenci0Özlem Hürmeydan1Asuman Orçun2Fatma Erdoğmuş3University of Health Sciences Türkiye, Kartal Dr. Lütfi Kırdar City Hospital, Biochemistry Laboratory, İstanbul, TürkiyeUniversity of Health Sciences Türkiye, Kartal Dr. Lütfi Kırdar City Hospital, Biochemistry Laboratory, İstanbul, TürkiyeUniversity of Health Sciences Türkiye, Kartal Dr. Lütfi Kırdar City Hospital, Biochemistry Laboratory, İstanbul, TürkiyeUniversity of Health Sciences Türkiye, Kartal Dr. Lütfi Kırdar City Hospital, Biochemistry Laboratory, İstanbul, TürkiyeObjective: The capillary zone electrophoresis (CZE) and highperformance liquid chromatography (HPLC) methods were compared in terms of HbA2 measurement for the assessment of hemoglobinopathies. Materials and Methods: CZE was compared with HPLC for the evaluation of patients without hemoglobinopathy (n=321), with β-thalassemia trait (n=113), and with common (HbD-Punjab, E, C, S/A, and S/S) and rare (HbS/D, O-Arap, Lepore, G-Coushata, Setif, Hamadan, Q-Iran, and H) variants (n=21). The reference range for HbA2 was determined by CZE. Results: Among patients without hemoglobinopathy, the median (2.5th-97.5th percentiles) values were 97.4% (97.0-98.0%) and 97.5% (96.6-98.4%) for HbA (p=0.060) and 2.4% (1.6-3.0%) and 2.5% (1.6-3.1%) for HbA2 (p<0.001) by HPLC and CZE, respectively. The reference range for HbA2 was 1.6-3.1% by CZE. In the comparison of methods for HbA2, there was a constant error of 0.255 (confidence interval: 0.062-0.448) and bias of 0.10% (limit of agreement: 0.33- 0.53), and higher values were obtained with CZE. A strong correlation was observed between the methods (r=0.782). Interrater agreement was almost perfect for clinical diagnosis (κ=0.911). The two methods detected and identified the common variants similarly. All rare variants, except HbH by HPLC and HbS/D by CZE, were detected as separate peaks by both methods. Conclusion: The two methods were in agreement regarding the preliminary identification of β-thalassemia patients. Different Hb variants were detected by both methods but with possible methodological interference for HbA2 measurements. CZE is a reliable and simple alternative for the evaluation of hemoglobinopathies. The standardization of HbA2 measurements should be prioritized as more techniques become available in routine laboratory practice.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-72593other hemoglobinopathiesthalassemiahemoglobin disordersanemia |
| spellingShingle | Özlem Çakır Madenci Özlem Hürmeydan Asuman Orçun Fatma Erdoğmuş Comparison of Capillary Zone Electrophoresis with High-pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies Turkish Journal of Hematology other hemoglobinopathies thalassemia hemoglobin disorders anemia |
| title | Comparison of Capillary Zone Electrophoresis with High-pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies |
| title_full | Comparison of Capillary Zone Electrophoresis with High-pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies |
| title_fullStr | Comparison of Capillary Zone Electrophoresis with High-pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies |
| title_full_unstemmed | Comparison of Capillary Zone Electrophoresis with High-pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies |
| title_short | Comparison of Capillary Zone Electrophoresis with High-pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies |
| title_sort | comparison of capillary zone electrophoresis with high pressure liquid chromatography in the evaluation of hemoglobinopathies |
| topic | other hemoglobinopathies thalassemia hemoglobin disorders anemia |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-72593 |
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