Single-Particle Tracking with Scanning Non-Linear Microscopy
This study describes the adaptation of non-linear microscopy for single-particle tracking (SPT), a method commonly used in biology with single-photon fluorescence. Imaging moving objects with non-linear microscopy raises difficulties due to the scanning process of the acquisitions. The interest of t...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/10/8/1519 |
| _version_ | 1797560388529684480 |
|---|---|
| author | Théo Travers Vincent G. Colin Matthieu Loumaigne Régis Barillé Denis Gindre |
| author_facet | Théo Travers Vincent G. Colin Matthieu Loumaigne Régis Barillé Denis Gindre |
| author_sort | Théo Travers |
| collection | DOAJ |
| description | This study describes the adaptation of non-linear microscopy for single-particle tracking (SPT), a method commonly used in biology with single-photon fluorescence. Imaging moving objects with non-linear microscopy raises difficulties due to the scanning process of the acquisitions. The interest of the study is based on the balance between all the experimental parameters (objective, resolution, frame rate) which need to be optimized to record long trajectories with the best accuracy and frame rate. To evaluate the performance of the setup for SPT, several basic estimation methods are used and adapted to the new detection process. The covariance-based estimator (CVE) seems to be the best way to evaluate the diffusion coefficient from trajectories using the specific factors of motion blur and localization error. |
| first_indexed | 2024-03-10T17:59:52Z |
| format | Article |
| id | doaj.art-43791133350e46fc9b23cdb607f4dfab |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T17:59:52Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-43791133350e46fc9b23cdb607f4dfab2023-11-20T08:57:16ZengMDPI AGNanomaterials2079-49912020-08-01108151910.3390/nano10081519Single-Particle Tracking with Scanning Non-Linear MicroscopyThéo Travers0Vincent G. Colin1Matthieu Loumaigne2Régis Barillé3Denis Gindre4MOLTECH-Anjou Laboratory, UMR CNRS 6200, University of Angers, 2 bd Lavoisier, 49045 Angers CEDEX, FranceMOLTECH-Anjou Laboratory, UMR CNRS 6200, University of Angers, 2 bd Lavoisier, 49045 Angers CEDEX, FranceMOLTECH-Anjou Laboratory, UMR CNRS 6200, University of Angers, 2 bd Lavoisier, 49045 Angers CEDEX, FranceMOLTECH-Anjou Laboratory, UMR CNRS 6200, University of Angers, 2 bd Lavoisier, 49045 Angers CEDEX, FranceMOLTECH-Anjou Laboratory, UMR CNRS 6200, University of Angers, 2 bd Lavoisier, 49045 Angers CEDEX, FranceThis study describes the adaptation of non-linear microscopy for single-particle tracking (SPT), a method commonly used in biology with single-photon fluorescence. Imaging moving objects with non-linear microscopy raises difficulties due to the scanning process of the acquisitions. The interest of the study is based on the balance between all the experimental parameters (objective, resolution, frame rate) which need to be optimized to record long trajectories with the best accuracy and frame rate. To evaluate the performance of the setup for SPT, several basic estimation methods are used and adapted to the new detection process. The covariance-based estimator (CVE) seems to be the best way to evaluate the diffusion coefficient from trajectories using the specific factors of motion blur and localization error.https://www.mdpi.com/2079-4991/10/8/1519non-linear microscopytwo-photon fluorescence imagingscanning microscopysingle particle trackingfree diffusionparticle size characterization |
| spellingShingle | Théo Travers Vincent G. Colin Matthieu Loumaigne Régis Barillé Denis Gindre Single-Particle Tracking with Scanning Non-Linear Microscopy Nanomaterials non-linear microscopy two-photon fluorescence imaging scanning microscopy single particle tracking free diffusion particle size characterization |
| title | Single-Particle Tracking with Scanning Non-Linear Microscopy |
| title_full | Single-Particle Tracking with Scanning Non-Linear Microscopy |
| title_fullStr | Single-Particle Tracking with Scanning Non-Linear Microscopy |
| title_full_unstemmed | Single-Particle Tracking with Scanning Non-Linear Microscopy |
| title_short | Single-Particle Tracking with Scanning Non-Linear Microscopy |
| title_sort | single particle tracking with scanning non linear microscopy |
| topic | non-linear microscopy two-photon fluorescence imaging scanning microscopy single particle tracking free diffusion particle size characterization |
| url | https://www.mdpi.com/2079-4991/10/8/1519 |
| work_keys_str_mv | AT theotravers singleparticletrackingwithscanningnonlinearmicroscopy AT vincentgcolin singleparticletrackingwithscanningnonlinearmicroscopy AT matthieuloumaigne singleparticletrackingwithscanningnonlinearmicroscopy AT regisbarille singleparticletrackingwithscanningnonlinearmicroscopy AT denisgindre singleparticletrackingwithscanningnonlinearmicroscopy |