Comparison Study of Two Fumonisin-Degrading Enzymes for Detoxification in Piglets
Fumonisins (FBs), particularly fumonisin B1 (FB1) and fumonisin B2 (FB2) produced mainly by <i>Fusarium verticillioide</i> and <i>Fusarium proliferatum</i>, are common contaminants in animal feed and pose a serious threat to both animal and human health. The use of microbial...
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MDPI AG
2023-12-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/16/1/3 |
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| author | Zhenlong Wang Zonghao Lv Tibor Czabany Veronika Nagl Rudolf Krska Xiumin Wang Bing Han Hui Tao Jie Liu Jinquan Wang |
| author_facet | Zhenlong Wang Zonghao Lv Tibor Czabany Veronika Nagl Rudolf Krska Xiumin Wang Bing Han Hui Tao Jie Liu Jinquan Wang |
| author_sort | Zhenlong Wang |
| collection | DOAJ |
| description | Fumonisins (FBs), particularly fumonisin B1 (FB1) and fumonisin B2 (FB2) produced mainly by <i>Fusarium verticillioide</i> and <i>Fusarium proliferatum</i>, are common contaminants in animal feed and pose a serious threat to both animal and human health. The use of microbial enzymes to efficiently and specifically convert fumonisins into non-toxic or low-toxic metabolites has emerged as the most promising approach. However, most of the available enzymes have only been evaluated in vitro and lack systematic evaluation in vivo. In this study, the detoxification efficacy of two carboxylesterases, FumD (FUM<i>zyme</i><sup>®</sup>) and FumDSB, was evaluated comparatively in piglets. The results show that feeding piglets 4.4 mg/kg FBs-contaminated diets for 32 days did not significantly affect the average daily gain, organ indices, and immunoglobulins of the piglets. However, a significant reduction (21.2%) in anti-inflammatory cytokine interleukin-4 was observed in the FBs group, and supplementation with FUM<i>zyme</i><sup>®</sup> and FumDSB significantly increased interleukin-4 by 62.1% and 28.0%, respectively. In addition, FBs-contaminated diets resulted in a 3-fold increase in the serum sphinganine/sphingosine (Sa/So) ratio, which is a specific biomarker that has been used to accurately reflect fumonisin levels. The serum Sa/So ratio was significantly reduced by 48.8% after the addition of FUM<i>zyme</i><sup>®</sup>, and was insignificantly reduced by 8.2% in the FumDSB group. These results suggested that FUM<i>zyme</i> was more effective than FumDSB in mitigating FBs toxicity in piglets by down-regulating the Sa/So ratio. |
| first_indexed | 2024-03-08T10:33:17Z |
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| id | doaj.art-437bbc8fd4c848c4945c9b890f78266f |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-08T10:33:17Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-437bbc8fd4c848c4945c9b890f78266f2024-01-26T18:42:08ZengMDPI AGToxins2072-66512023-12-01161310.3390/toxins16010003Comparison Study of Two Fumonisin-Degrading Enzymes for Detoxification in PigletsZhenlong Wang0Zonghao Lv1Tibor Czabany2Veronika Nagl3Rudolf Krska4Xiumin Wang5Bing Han6Hui Tao7Jie Liu8Jinquan Wang9Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, Chinadsm-firmenich, Animal Nutrition and Health R&D Center, Technopark 1, 3430 Tulln, Austriadsm-firmenich, Animal Nutrition and Health R&D Center, Technopark 1, 3430 Tulln, AustriaDepartment of Agrobiotechnology IFA-Tulln, Institute of Bioanalytics and Agro-Metabolomics, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Str. 20, 3430 Tulln, AustriaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, ChinaFumonisins (FBs), particularly fumonisin B1 (FB1) and fumonisin B2 (FB2) produced mainly by <i>Fusarium verticillioide</i> and <i>Fusarium proliferatum</i>, are common contaminants in animal feed and pose a serious threat to both animal and human health. The use of microbial enzymes to efficiently and specifically convert fumonisins into non-toxic or low-toxic metabolites has emerged as the most promising approach. However, most of the available enzymes have only been evaluated in vitro and lack systematic evaluation in vivo. In this study, the detoxification efficacy of two carboxylesterases, FumD (FUM<i>zyme</i><sup>®</sup>) and FumDSB, was evaluated comparatively in piglets. The results show that feeding piglets 4.4 mg/kg FBs-contaminated diets for 32 days did not significantly affect the average daily gain, organ indices, and immunoglobulins of the piglets. However, a significant reduction (21.2%) in anti-inflammatory cytokine interleukin-4 was observed in the FBs group, and supplementation with FUM<i>zyme</i><sup>®</sup> and FumDSB significantly increased interleukin-4 by 62.1% and 28.0%, respectively. In addition, FBs-contaminated diets resulted in a 3-fold increase in the serum sphinganine/sphingosine (Sa/So) ratio, which is a specific biomarker that has been used to accurately reflect fumonisin levels. The serum Sa/So ratio was significantly reduced by 48.8% after the addition of FUM<i>zyme</i><sup>®</sup>, and was insignificantly reduced by 8.2% in the FumDSB group. These results suggested that FUM<i>zyme</i> was more effective than FumDSB in mitigating FBs toxicity in piglets by down-regulating the Sa/So ratio.https://www.mdpi.com/2072-6651/16/1/3fumonisinsSa/SoFumDSBFUM<i>zyme</i>degrading enzyme |
| spellingShingle | Zhenlong Wang Zonghao Lv Tibor Czabany Veronika Nagl Rudolf Krska Xiumin Wang Bing Han Hui Tao Jie Liu Jinquan Wang Comparison Study of Two Fumonisin-Degrading Enzymes for Detoxification in Piglets Toxins fumonisins Sa/So FumDSB FUM<i>zyme</i> degrading enzyme |
| title | Comparison Study of Two Fumonisin-Degrading Enzymes for Detoxification in Piglets |
| title_full | Comparison Study of Two Fumonisin-Degrading Enzymes for Detoxification in Piglets |
| title_fullStr | Comparison Study of Two Fumonisin-Degrading Enzymes for Detoxification in Piglets |
| title_full_unstemmed | Comparison Study of Two Fumonisin-Degrading Enzymes for Detoxification in Piglets |
| title_short | Comparison Study of Two Fumonisin-Degrading Enzymes for Detoxification in Piglets |
| title_sort | comparison study of two fumonisin degrading enzymes for detoxification in piglets |
| topic | fumonisins Sa/So FumDSB FUM<i>zyme</i> degrading enzyme |
| url | https://www.mdpi.com/2072-6651/16/1/3 |
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