Short-chain fluorescent tryptophan tags for on-line detection of functional recombinant proteins

Short-chain fluorescent tryptophan tags for on-line detection of functional recombinant proteins

<p>Abstract</p> <p>Background</p> <p>Conventional fluorescent proteins, such as GFP, its derivatives and flavin mononucleotide based fluorescent proteins (FbFPs) are often used as fusion tags for detecting recombinant proteins during cultivation. These reporter tags are...

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Bibliographic Details
Main Authors: Siepert Eva-Maria, Gartz Esther, Tur Mehmet Kemal, Delbrück Heinrich, Barth Stefan, Büchs Jochen
Format: Article
Language:English
Published: BMC 2012-09-01
Series:BMC Biotechnology
Subjects:
Tryptophan tag
On-line monitoring
Microtiter plate
Fluorescence measurement
<it>Escherichia coli</it> protein expression
Small scale fermentation
Online Access:http://www.biomedcentral.com/1472-6750/12/65

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