Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice
Commensal microbiota contribute to gut homeostasis by inducing transcription of mucosal genes. Analysis of the impact of various microbiota on intestinal tissue provides an important insight into the function of this organ. We used cDNA microarrays to determine the gene expression signature of mucos...
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MDPI AG
2019-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/20/7/1581 |
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| author | Lucie Janeckova Klara Kostovcikova Jiri Svec Monika Stastna Hynek Strnad Michal Kolar Tomas Hudcovic Jitka Stancikova Jolana Tureckova Nikol Baloghova Eva Sloncova Katerina Galuskova Helena Tlaskalova-Hogenova Vladimir Korinek |
| author_facet | Lucie Janeckova Klara Kostovcikova Jiri Svec Monika Stastna Hynek Strnad Michal Kolar Tomas Hudcovic Jitka Stancikova Jolana Tureckova Nikol Baloghova Eva Sloncova Katerina Galuskova Helena Tlaskalova-Hogenova Vladimir Korinek |
| author_sort | Lucie Janeckova |
| collection | DOAJ |
| description | Commensal microbiota contribute to gut homeostasis by inducing transcription of mucosal genes. Analysis of the impact of various microbiota on intestinal tissue provides an important insight into the function of this organ. We used cDNA microarrays to determine the gene expression signature of mucosa isolated from the small intestine and colon of germ-free (GF) mice and animals monoassociated with two <i>E. coli</i> strains. The results were compared to the expression data obtained in conventionally reared (CR) mice. In addition, we analyzed gene expression in colon organoids derived from CR, GF, and monoassociated animals. The analysis revealed that the complete absence of intestinal microbiota mainly affected the mucosal immune system, which was not restored upon monoassociation. The most important expression changes observed in the colon mucosa indicated alterations in adipose tissue and lipid metabolism. In the comparison of differentially expressed genes in the mucosa or organoids obtained from GF and CR mice, only six genes were common for both types of samples. The results show that the increased expression of the angiopoietin-like 4 (<i>Angptl4</i>) gene encoding a secreted regulator of lipid metabolism indicates the GF status. |
| first_indexed | 2024-04-13T03:52:08Z |
| format | Article |
| id | doaj.art-4381e4a0117f43d5a72f3e81a0689f86 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T03:52:08Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4381e4a0117f43d5a72f3e81a0689f862022-12-22T03:03:46ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-03-01207158110.3390/ijms20071581ijms20071581Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated MiceLucie Janeckova0Klara Kostovcikova1Jiri Svec2Monika Stastna3Hynek Strnad4Michal Kolar5Tomas Hudcovic6Jitka Stancikova7Jolana Tureckova8Nikol Baloghova9Eva Sloncova10Katerina Galuskova11Helena Tlaskalova-Hogenova12Vladimir Korinek13Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech RepublicCommensal microbiota contribute to gut homeostasis by inducing transcription of mucosal genes. Analysis of the impact of various microbiota on intestinal tissue provides an important insight into the function of this organ. We used cDNA microarrays to determine the gene expression signature of mucosa isolated from the small intestine and colon of germ-free (GF) mice and animals monoassociated with two <i>E. coli</i> strains. The results were compared to the expression data obtained in conventionally reared (CR) mice. In addition, we analyzed gene expression in colon organoids derived from CR, GF, and monoassociated animals. The analysis revealed that the complete absence of intestinal microbiota mainly affected the mucosal immune system, which was not restored upon monoassociation. The most important expression changes observed in the colon mucosa indicated alterations in adipose tissue and lipid metabolism. In the comparison of differentially expressed genes in the mucosa or organoids obtained from GF and CR mice, only six genes were common for both types of samples. The results show that the increased expression of the angiopoietin-like 4 (<i>Angptl4</i>) gene encoding a secreted regulator of lipid metabolism indicates the GF status.https://www.mdpi.com/1422-0067/20/7/1581expression profilingEnricher toolmicrobiotamonoassociationOnecut2 |
| spellingShingle | Lucie Janeckova Klara Kostovcikova Jiri Svec Monika Stastna Hynek Strnad Michal Kolar Tomas Hudcovic Jitka Stancikova Jolana Tureckova Nikol Baloghova Eva Sloncova Katerina Galuskova Helena Tlaskalova-Hogenova Vladimir Korinek Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice International Journal of Molecular Sciences expression profiling Enricher tool microbiota monoassociation Onecut2 |
| title | Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice |
| title_full | Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice |
| title_fullStr | Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice |
| title_full_unstemmed | Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice |
| title_short | Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice |
| title_sort | unique gene expression signatures in the intestinal mucosa and organoids derived from germ free and monoassociated mice |
| topic | expression profiling Enricher tool microbiota monoassociation Onecut2 |
| url | https://www.mdpi.com/1422-0067/20/7/1581 |
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