Hydroxyapatite and Titanium Dioxide Nanoparticles: Radiolabelling and In Vitro Stability of Prospective Theranostic Nanocarriers for <sup>223</sup>Ra and <sup>99m</sup>Tc
Hydroxyapatite and titanium dioxide are widely used materials in a broad spectrum of branches. Due to their appropriate properties such as a large specific surface area, radiation stability or relatively low toxicity, they could be potentially used as nanocarriers for medicinal radionuclides for dia...
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MDPI AG
2020-08-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/9/1632 |
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| author | Petra Suchánková Ekaterina Kukleva Eva Nykl Pavel Nykl Michal Sakmár Martin Vlk Ján Kozempel |
| author_facet | Petra Suchánková Ekaterina Kukleva Eva Nykl Pavel Nykl Michal Sakmár Martin Vlk Ján Kozempel |
| author_sort | Petra Suchánková |
| collection | DOAJ |
| description | Hydroxyapatite and titanium dioxide are widely used materials in a broad spectrum of branches. Due to their appropriate properties such as a large specific surface area, radiation stability or relatively low toxicity, they could be potentially used as nanocarriers for medicinal radionuclides for diagnostics and therapy. Two radiolabelling strategies of both nanomaterials were carried out by <sup>99m</sup>Tc for diagnostic purposes and by <sup>223</sup>Ra for therapeutic purposes. The first one was the radionuclide sorption on ready-made nanoparticles and the second one was direct radionuclide incorporation into the structure of the nanoparticles. Achieved labelling yields were higher than 94% in all cases. Afterwards, in vitro stability tests were carried out in several solutions: physiological saline, bovine blood plasma, bovine blood serum, 1% and 5% human albumin solutions. In vitro stability studies were performed as short-term (59 h for <sup>223</sup>Ra and 31 h for <sup>99m</sup>Tc) and long-term experiments (five half-lives of <sup>223</sup>Ra, approx. 55 days). Both radiolabelled nanoparticles with <sup>99m</sup>Tc have shown similar released activities (about 20%) in all solutions. The best results were obtained for <sup>223</sup>Ra radiolabelled titanium dioxide nanoparticles, where overall released activities were under 6% for 59 h study in all matrices and under 3% for 55 days in a long-term perspective. |
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| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T17:08:38Z |
| publishDate | 2020-08-01 |
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| series | Nanomaterials |
| spelling | doaj.art-4383af224d0241e98666ee09b9890dbc2023-11-20T10:44:06ZengMDPI AGNanomaterials2079-49912020-08-01109163210.3390/nano10091632Hydroxyapatite and Titanium Dioxide Nanoparticles: Radiolabelling and In Vitro Stability of Prospective Theranostic Nanocarriers for <sup>223</sup>Ra and <sup>99m</sup>TcPetra Suchánková0Ekaterina Kukleva1Eva Nykl2Pavel Nykl3Michal Sakmár4Martin Vlk5Ján Kozempel6Department of Nuclear Chemistry, Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Břehová 7, 11519 Prague 1, Czech RepublicDepartment of Nuclear Chemistry, Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Břehová 7, 11519 Prague 1, Czech RepublicDepartment of Nuclear Chemistry, Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Břehová 7, 11519 Prague 1, Czech RepublicDepartment of Nuclear Chemistry, Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Břehová 7, 11519 Prague 1, Czech RepublicDepartment of Nuclear Chemistry, Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Břehová 7, 11519 Prague 1, Czech RepublicDepartment of Nuclear Chemistry, Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Břehová 7, 11519 Prague 1, Czech RepublicDepartment of Nuclear Chemistry, Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Břehová 7, 11519 Prague 1, Czech RepublicHydroxyapatite and titanium dioxide are widely used materials in a broad spectrum of branches. Due to their appropriate properties such as a large specific surface area, radiation stability or relatively low toxicity, they could be potentially used as nanocarriers for medicinal radionuclides for diagnostics and therapy. Two radiolabelling strategies of both nanomaterials were carried out by <sup>99m</sup>Tc for diagnostic purposes and by <sup>223</sup>Ra for therapeutic purposes. The first one was the radionuclide sorption on ready-made nanoparticles and the second one was direct radionuclide incorporation into the structure of the nanoparticles. Achieved labelling yields were higher than 94% in all cases. Afterwards, in vitro stability tests were carried out in several solutions: physiological saline, bovine blood plasma, bovine blood serum, 1% and 5% human albumin solutions. In vitro stability studies were performed as short-term (59 h for <sup>223</sup>Ra and 31 h for <sup>99m</sup>Tc) and long-term experiments (five half-lives of <sup>223</sup>Ra, approx. 55 days). Both radiolabelled nanoparticles with <sup>99m</sup>Tc have shown similar released activities (about 20%) in all solutions. The best results were obtained for <sup>223</sup>Ra radiolabelled titanium dioxide nanoparticles, where overall released activities were under 6% for 59 h study in all matrices and under 3% for 55 days in a long-term perspective.https://www.mdpi.com/2079-4991/10/9/1632hydroxyapatitetitanium dioxidenanoparticlesradium<sup>223</sup>Ratechnetium |
| spellingShingle | Petra Suchánková Ekaterina Kukleva Eva Nykl Pavel Nykl Michal Sakmár Martin Vlk Ján Kozempel Hydroxyapatite and Titanium Dioxide Nanoparticles: Radiolabelling and In Vitro Stability of Prospective Theranostic Nanocarriers for <sup>223</sup>Ra and <sup>99m</sup>Tc Nanomaterials hydroxyapatite titanium dioxide nanoparticles radium <sup>223</sup>Ra technetium |
| title | Hydroxyapatite and Titanium Dioxide Nanoparticles: Radiolabelling and In Vitro Stability of Prospective Theranostic Nanocarriers for <sup>223</sup>Ra and <sup>99m</sup>Tc |
| title_full | Hydroxyapatite and Titanium Dioxide Nanoparticles: Radiolabelling and In Vitro Stability of Prospective Theranostic Nanocarriers for <sup>223</sup>Ra and <sup>99m</sup>Tc |
| title_fullStr | Hydroxyapatite and Titanium Dioxide Nanoparticles: Radiolabelling and In Vitro Stability of Prospective Theranostic Nanocarriers for <sup>223</sup>Ra and <sup>99m</sup>Tc |
| title_full_unstemmed | Hydroxyapatite and Titanium Dioxide Nanoparticles: Radiolabelling and In Vitro Stability of Prospective Theranostic Nanocarriers for <sup>223</sup>Ra and <sup>99m</sup>Tc |
| title_short | Hydroxyapatite and Titanium Dioxide Nanoparticles: Radiolabelling and In Vitro Stability of Prospective Theranostic Nanocarriers for <sup>223</sup>Ra and <sup>99m</sup>Tc |
| title_sort | hydroxyapatite and titanium dioxide nanoparticles radiolabelling and in vitro stability of prospective theranostic nanocarriers for sup 223 sup ra and sup 99m sup tc |
| topic | hydroxyapatite titanium dioxide nanoparticles radium <sup>223</sup>Ra technetium |
| url | https://www.mdpi.com/2079-4991/10/9/1632 |
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