Genotypic diversity of Streptococcus mutans isolated from preschoolers with and without early dental care
Abstract Introduction Streptococcus mutans (S. mutans) exhibits extensive genotypic diversity, but the role of this variation is poorly understood. Objective To evaluate the genotypic diversity of S. mutans in preschoolers with and without early dental care and to associate it with dental caries e...
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| Format: | Article |
| Language: | English |
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Universidade Estadual Paulista
2019-06-01
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| Series: | Revista de Odontologia da UNESP |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772019000100407&tlng=en |
| _version_ | 1798021767868973056 |
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| author | Ana Lídia Soares COTA Janaína Accordi JUNKES Regina Célia POLI-FREDERICO |
| author_facet | Ana Lídia Soares COTA Janaína Accordi JUNKES Regina Célia POLI-FREDERICO |
| author_sort | Ana Lídia Soares COTA |
| collection | DOAJ |
| description | Abstract Introduction Streptococcus mutans (S. mutans) exhibits extensive genotypic diversity, but the role of this variation is poorly understood. Objective To evaluate the genotypic diversity of S. mutans in preschoolers with and without early dental care and to associate it with dental caries experience. Material and method Twenty children, aged five year old, with history of dental care since they were one year old (G1) and 20 children without history of dental care (G2) were included. Their oral health status was assessed by the dmft index. S. mutans samples were isolated from the saliva and analysed by polymerase chain reaction. A total of 339 S. mutans isolates (173 from G1 and 166 from G2) were genotyped by random amplified polymorphic DNA with arbitrary primers OPA-02 and OPA-13. Result The results revealed 75 distinct genotypes of S. mutans in G1 and 73 in G2. Furthermore, G1 and G2 subjects harboured one to eight and one to seven distinct genotypes of S. mutans, respectively. A statistically significant association (P=0.021) and a moderate correlation (r= 0.503) were observed between dental caries experience and genotypic diversity of S. mutans in G1. Conclusion From the limitations of the study design, we just can hypothesize that actions of early dental care carried out by an educative-preventive program can contribute to a distinct oral microbiota. |
| first_indexed | 2024-04-11T17:18:57Z |
| format | Article |
| id | doaj.art-4383d3acd36840da80b531bb7bde330f |
| institution | Directory Open Access Journal |
| issn | 1807-2577 |
| language | English |
| last_indexed | 2024-04-11T17:18:57Z |
| publishDate | 2019-06-01 |
| publisher | Universidade Estadual Paulista |
| record_format | Article |
| series | Revista de Odontologia da UNESP |
| spelling | doaj.art-4383d3acd36840da80b531bb7bde330f2022-12-22T04:12:32ZengUniversidade Estadual PaulistaRevista de Odontologia da UNESP1807-25772019-06-014810.1590/1807-2577.08018Genotypic diversity of Streptococcus mutans isolated from preschoolers with and without early dental careAna Lídia Soares COTAhttps://orcid.org/0000-0001-8220-7846Janaína Accordi JUNKEShttps://orcid.org/0000-0002-4442-8856Regina Célia POLI-FREDERICOhttps://orcid.org/0000-0003-4631-4606Abstract Introduction Streptococcus mutans (S. mutans) exhibits extensive genotypic diversity, but the role of this variation is poorly understood. Objective To evaluate the genotypic diversity of S. mutans in preschoolers with and without early dental care and to associate it with dental caries experience. Material and method Twenty children, aged five year old, with history of dental care since they were one year old (G1) and 20 children without history of dental care (G2) were included. Their oral health status was assessed by the dmft index. S. mutans samples were isolated from the saliva and analysed by polymerase chain reaction. A total of 339 S. mutans isolates (173 from G1 and 166 from G2) were genotyped by random amplified polymorphic DNA with arbitrary primers OPA-02 and OPA-13. Result The results revealed 75 distinct genotypes of S. mutans in G1 and 73 in G2. Furthermore, G1 and G2 subjects harboured one to eight and one to seven distinct genotypes of S. mutans, respectively. A statistically significant association (P=0.021) and a moderate correlation (r= 0.503) were observed between dental caries experience and genotypic diversity of S. mutans in G1. Conclusion From the limitations of the study design, we just can hypothesize that actions of early dental care carried out by an educative-preventive program can contribute to a distinct oral microbiota.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772019000100407&tlng=enGenotypic diversityStreptococcus mutansdental caredental caries |
| spellingShingle | Ana Lídia Soares COTA Janaína Accordi JUNKES Regina Célia POLI-FREDERICO Genotypic diversity of Streptococcus mutans isolated from preschoolers with and without early dental care Revista de Odontologia da UNESP Genotypic diversity Streptococcus mutans dental care dental caries |
| title | Genotypic diversity of Streptococcus mutans isolated from preschoolers with and without early dental care |
| title_full | Genotypic diversity of Streptococcus mutans isolated from preschoolers with and without early dental care |
| title_fullStr | Genotypic diversity of Streptococcus mutans isolated from preschoolers with and without early dental care |
| title_full_unstemmed | Genotypic diversity of Streptococcus mutans isolated from preschoolers with and without early dental care |
| title_short | Genotypic diversity of Streptococcus mutans isolated from preschoolers with and without early dental care |
| title_sort | genotypic diversity of streptococcus mutans isolated from preschoolers with and without early dental care |
| topic | Genotypic diversity Streptococcus mutans dental care dental caries |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772019000100407&tlng=en |
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