Studies on immunopathological changes induced by commercial IBD live vaccines in poultry birds
Abstract Intermediate plus live strain infectious bursal disease virus (IBDV) vaccines are used to control IBDV endemic infections in India. In the present study, immunopathological changes induced by commercial infectious bursal disease live vaccines with different immunization regimes were compare...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-39017-5 |
| _version_ | 1827875520590643200 |
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| author | Sushma Kajal Gulshan Narang Babu Lal Jangir Pooja Kundu Deepika Lather Rajesh Chhabra |
| author_facet | Sushma Kajal Gulshan Narang Babu Lal Jangir Pooja Kundu Deepika Lather Rajesh Chhabra |
| author_sort | Sushma Kajal |
| collection | DOAJ |
| description | Abstract Intermediate plus live strain infectious bursal disease virus (IBDV) vaccines are used to control IBDV endemic infections in India. In the present study, immunopathological changes induced by commercial infectious bursal disease live vaccines with different immunization regimes were compared. A total of days old 108 Cobb broiler chicks were randomly divided into five groups with 24 chicks each in groups I, II, III and 18 chicks each in group IV and V. Group I served as control I (no immunization) and group II and III chicks were immunized with a single dose of vaccines 1 and 2 on 17th day of age (DOA), respectively. The group IV and V chicks were immunized with vaccines 1 and 2, respectively with primary dose on 17th DOA followed by booster dose on 24th DOA. Both intermediate plus live vaccines produced gross and histopathological lesions in lymphoid organs (bursa of Fabricius, thymus, spleen and caecal tonsils). Increased CD4 + , CD8 + T cells in affected bursa of Fabricius was evidenced by immunohistochemistry. Further, up-regulation in relative mRNA expression of IFN-γ, IL-1β and IL-6 were observed in bursa of Fabricius of treated birds, with maximum alteration particularly on 14th day post single immunization and 7th day post booster immunization. The findings suggest that single immunization regime on the 17th day of age showed immunization equivalent to booster immunization with lesser lesions, therefore, may be practiced and promoted in the field conditions for the better economic returns and animal welfare. |
| first_indexed | 2024-03-12T17:08:07Z |
| format | Article |
| id | doaj.art-4386d38deef44327b67796c24c2f9b00 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T17:08:07Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4386d38deef44327b67796c24c2f9b002023-08-06T11:14:08ZengNature PortfolioScientific Reports2045-23222023-07-0113111010.1038/s41598-023-39017-5Studies on immunopathological changes induced by commercial IBD live vaccines in poultry birdsSushma Kajal0Gulshan Narang1Babu Lal Jangir2Pooja Kundu3Deepika Lather4Rajesh Chhabra5Department of Veterinary Pathology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal SciencesDepartment of Veterinary Pathology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal SciencesDepartment of Veterinary Pathology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal SciencesDepartment of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal SciencesDepartment of Veterinary Pathology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal SciencesCollege Central Laboratory, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal SciencesAbstract Intermediate plus live strain infectious bursal disease virus (IBDV) vaccines are used to control IBDV endemic infections in India. In the present study, immunopathological changes induced by commercial infectious bursal disease live vaccines with different immunization regimes were compared. A total of days old 108 Cobb broiler chicks were randomly divided into five groups with 24 chicks each in groups I, II, III and 18 chicks each in group IV and V. Group I served as control I (no immunization) and group II and III chicks were immunized with a single dose of vaccines 1 and 2 on 17th day of age (DOA), respectively. The group IV and V chicks were immunized with vaccines 1 and 2, respectively with primary dose on 17th DOA followed by booster dose on 24th DOA. Both intermediate plus live vaccines produced gross and histopathological lesions in lymphoid organs (bursa of Fabricius, thymus, spleen and caecal tonsils). Increased CD4 + , CD8 + T cells in affected bursa of Fabricius was evidenced by immunohistochemistry. Further, up-regulation in relative mRNA expression of IFN-γ, IL-1β and IL-6 were observed in bursa of Fabricius of treated birds, with maximum alteration particularly on 14th day post single immunization and 7th day post booster immunization. The findings suggest that single immunization regime on the 17th day of age showed immunization equivalent to booster immunization with lesser lesions, therefore, may be practiced and promoted in the field conditions for the better economic returns and animal welfare.https://doi.org/10.1038/s41598-023-39017-5 |
| spellingShingle | Sushma Kajal Gulshan Narang Babu Lal Jangir Pooja Kundu Deepika Lather Rajesh Chhabra Studies on immunopathological changes induced by commercial IBD live vaccines in poultry birds Scientific Reports |
| title | Studies on immunopathological changes induced by commercial IBD live vaccines in poultry birds |
| title_full | Studies on immunopathological changes induced by commercial IBD live vaccines in poultry birds |
| title_fullStr | Studies on immunopathological changes induced by commercial IBD live vaccines in poultry birds |
| title_full_unstemmed | Studies on immunopathological changes induced by commercial IBD live vaccines in poultry birds |
| title_short | Studies on immunopathological changes induced by commercial IBD live vaccines in poultry birds |
| title_sort | studies on immunopathological changes induced by commercial ibd live vaccines in poultry birds |
| url | https://doi.org/10.1038/s41598-023-39017-5 |
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