Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L.
Sesame Fusarium wilt (SFW), caused by Fusarium oxysporum f. sp. sesami (Fos), is one of the most devastating diseases affecting sesame cultivation. Deciphering the genetic control of SFW resistance is pivotal for effective disease management in sesame. An inheritance study on a cross between the hig...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-02-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514124000072 |
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| author | Yinghui Duan Wenwen Qu Shuxian Chang Ming Ju Cuiying Wang Cong Mu Hengchun Cao Guiting Li Qiuzhen Tian Qin Ma Zhanyou Zhang Haiyang Zhang Hongmei Miao |
| author_facet | Yinghui Duan Wenwen Qu Shuxian Chang Ming Ju Cuiying Wang Cong Mu Hengchun Cao Guiting Li Qiuzhen Tian Qin Ma Zhanyou Zhang Haiyang Zhang Hongmei Miao |
| author_sort | Yinghui Duan |
| collection | DOAJ |
| description | Sesame Fusarium wilt (SFW), caused by Fusarium oxysporum f. sp. sesami (Fos), is one of the most devastating diseases affecting sesame cultivation. Deciphering the genetic control of SFW resistance is pivotal for effective disease management in sesame. An inheritance study on a cross between the highly resistant variety Yuzhi 11 and the highly susceptible accession Sp1 using a Fos pathogenicity group 1 isolate indicated that resistance was conferred by a single dominant allele. The target locus was located in a 1.24 Mb interval on chromosome 3 using a combination of cross-population association mapping and bulked segregant analysis. Fine genetic mapping further narrowed the interval between 21,350 and 21,401 kb. The locus Sindi_0812400 was identified as the SFW resistance gene and officially designated SiRLK1. This gene encodes a specific malectin/receptor-like protein kinase with three putative tandem kinase domains and is considered a kinase fusion protein. Sequence analysis revealed that a high proportion (49.44%) of variants within the locus was located within the kinase domain III, and several of which were evidently associated with the diversity in SFW response, indicating the critical role of kinase domain III in expression of disease resistance. These findings provide valuable information for further functional analysis of SFW resistance genes and marker-assisted resistance breeding in sesame. |
| first_indexed | 2024-03-07T19:09:12Z |
| format | Article |
| id | doaj.art-43890d95fc0d42bcb694b98a0e1a4057 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-03-07T19:09:12Z |
| publishDate | 2024-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-43890d95fc0d42bcb694b98a0e1a40572024-03-01T05:06:26ZengKeAi Communications Co., Ltd.Crop Journal2214-51412024-02-01121252261Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L.Yinghui Duan0Wenwen Qu1Shuxian Chang2Ming Ju3Cuiying Wang4Cong Mu5Hengchun Cao6Guiting Li7Qiuzhen Tian8Qin Ma9Zhanyou Zhang10Haiyang Zhang11Hongmei Miao12The Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, ChinaThe Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, ChinaThe Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, ChinaThe Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, ChinaThe Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, ChinaThe Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, ChinaThe Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, ChinaThe Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, ChinaThe Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, ChinaThe Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, ChinaThe Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, ChinaThe Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Corresponding authors.The Shennong Laboratory, Zhengzhou 450002, Henan, China; Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Key Laboratory of Specific Oilseed Crops Genomics of Henan Province, Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; Corresponding authors.Sesame Fusarium wilt (SFW), caused by Fusarium oxysporum f. sp. sesami (Fos), is one of the most devastating diseases affecting sesame cultivation. Deciphering the genetic control of SFW resistance is pivotal for effective disease management in sesame. An inheritance study on a cross between the highly resistant variety Yuzhi 11 and the highly susceptible accession Sp1 using a Fos pathogenicity group 1 isolate indicated that resistance was conferred by a single dominant allele. The target locus was located in a 1.24 Mb interval on chromosome 3 using a combination of cross-population association mapping and bulked segregant analysis. Fine genetic mapping further narrowed the interval between 21,350 and 21,401 kb. The locus Sindi_0812400 was identified as the SFW resistance gene and officially designated SiRLK1. This gene encodes a specific malectin/receptor-like protein kinase with three putative tandem kinase domains and is considered a kinase fusion protein. Sequence analysis revealed that a high proportion (49.44%) of variants within the locus was located within the kinase domain III, and several of which were evidently associated with the diversity in SFW response, indicating the critical role of kinase domain III in expression of disease resistance. These findings provide valuable information for further functional analysis of SFW resistance genes and marker-assisted resistance breeding in sesame.http://www.sciencedirect.com/science/article/pii/S2214514124000072Breeding for resistanceGenetic analysisMolecular analysisTandem kinase domain |
| spellingShingle | Yinghui Duan Wenwen Qu Shuxian Chang Ming Ju Cuiying Wang Cong Mu Hengchun Cao Guiting Li Qiuzhen Tian Qin Ma Zhanyou Zhang Haiyang Zhang Hongmei Miao Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L. Crop Journal Breeding for resistance Genetic analysis Molecular analysis Tandem kinase domain |
| title | Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L. |
| title_full | Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L. |
| title_fullStr | Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L. |
| title_full_unstemmed | Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L. |
| title_short | Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L. |
| title_sort | identification of fusarium wilt resistance gene sirlk1 in sesamum indicum l |
| topic | Breeding for resistance Genetic analysis Molecular analysis Tandem kinase domain |
| url | http://www.sciencedirect.com/science/article/pii/S2214514124000072 |
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