In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein
Abstract Objective In vitro fertilization-embryo transfer (IVF-ET) technologies (especially frozen ET) have been widely used, which might affect maternal and fetal health. Information regarding influence of IVF-ET on the vasoconstriction of human umbilical vein (HUV) is limited. This study determine...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12958-023-01103-8 |
| _version_ | 1797801167806267392 |
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| author | Jiaqi Tang Linglu Qi Yun He Na Li Ze Zhang Xiuwen Zhou Hongyu Su Qiutong Zheng Yumeng Zhang Jianying Tao Zhice Xu |
| author_facet | Jiaqi Tang Linglu Qi Yun He Na Li Ze Zhang Xiuwen Zhou Hongyu Su Qiutong Zheng Yumeng Zhang Jianying Tao Zhice Xu |
| author_sort | Jiaqi Tang |
| collection | DOAJ |
| description | Abstract Objective In vitro fertilization-embryo transfer (IVF-ET) technologies (especially frozen ET) have been widely used, which might affect maternal and fetal health. Information regarding influence of IVF-ET on the vasoconstriction of human umbilical vein (HUV) is limited. This study determined effects of frozen ET on histamine-mediated vascular responses in HUV and related mechanisms. Methods and results HUVs were collected from frozen ET conceived pregnancy and spontaneously conceived pregnancy (control). Histamine concentration in umbilical plasma was higher in frozen ET group than the control. Histamine-mediated contractile response curve was left-shifted in the frozen ET group when comparing with the control. In isolated HUV rings, H1R showed a critical role in regulating vascular constriction, while H2R played little roles in regulating vessel tone. Iberiotoxin and 4-aminopyridine didn’t significantly change histamine-mediated constriction in HUVs. Histamine-induced vasoconstrictions were significantly decreased by nifedipine, KN93, or GF109203X, while the inhibitory effects were significantly greater in the frozen ET group in comparison to the control. The constrictions by Bay K8644, phenylephrine, or PDBu were stronger in frozen ET, respectively. There was a decrease in the protein expressions of H1R and H2R, an increase in protein expressions of BKCaα and PKCβ. Conclusions Histamine-induced constriction in HUV was mainly via H1R. The increased sensitivity to histamine in HUV following frozen ET cycles were linked to the enhanced PKCβ protein expression and function. The new data and findings in this study provide important insight into influences of frozen ET on fetal vessel development and potential influence in long-term. |
| first_indexed | 2024-03-13T04:46:19Z |
| format | Article |
| id | doaj.art-438de39312824d93bf2a70e8b69a828f |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-03-13T04:46:19Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-438de39312824d93bf2a70e8b69a828f2023-06-18T11:28:20ZengBMCReproductive Biology and Endocrinology1477-78272023-06-0121111210.1186/s12958-023-01103-8In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical veinJiaqi Tang0Linglu Qi1Yun He2Na Li3Ze Zhang4Xiuwen Zhou5Hongyu Su6Qiutong Zheng7Yumeng Zhang8Jianying Tao9Zhice Xu10Institute for Fetology, First Hospital of Soochow UniversityWomen’s Hospital School of Medicine Zhejiang UniversityDepartment of Obstetrics and Gynecology, Taixing People’s HospitalMaternal and Child Health Care Hospital of WuxiInstitute for Fetology, First Hospital of Soochow UniversityInstitute for Fetology, First Hospital of Soochow UniversityInstitute for Fetology, First Hospital of Soochow UniversityInstitute for Fetology, First Hospital of Soochow UniversityInstitute for Fetology, First Hospital of Soochow UniversitySuzhou Municipal Hospital, the Affiliated Suzhou Hospital of Nanjing Medical UniversityInstitute for Fetology, First Hospital of Soochow UniversityAbstract Objective In vitro fertilization-embryo transfer (IVF-ET) technologies (especially frozen ET) have been widely used, which might affect maternal and fetal health. Information regarding influence of IVF-ET on the vasoconstriction of human umbilical vein (HUV) is limited. This study determined effects of frozen ET on histamine-mediated vascular responses in HUV and related mechanisms. Methods and results HUVs were collected from frozen ET conceived pregnancy and spontaneously conceived pregnancy (control). Histamine concentration in umbilical plasma was higher in frozen ET group than the control. Histamine-mediated contractile response curve was left-shifted in the frozen ET group when comparing with the control. In isolated HUV rings, H1R showed a critical role in regulating vascular constriction, while H2R played little roles in regulating vessel tone. Iberiotoxin and 4-aminopyridine didn’t significantly change histamine-mediated constriction in HUVs. Histamine-induced vasoconstrictions were significantly decreased by nifedipine, KN93, or GF109203X, while the inhibitory effects were significantly greater in the frozen ET group in comparison to the control. The constrictions by Bay K8644, phenylephrine, or PDBu were stronger in frozen ET, respectively. There was a decrease in the protein expressions of H1R and H2R, an increase in protein expressions of BKCaα and PKCβ. Conclusions Histamine-induced constriction in HUV was mainly via H1R. The increased sensitivity to histamine in HUV following frozen ET cycles were linked to the enhanced PKCβ protein expression and function. The new data and findings in this study provide important insight into influences of frozen ET on fetal vessel development and potential influence in long-term.https://doi.org/10.1186/s12958-023-01103-8Frozen embryo transferHistamineIn vitro fertilizationUmbilical veinVascular tension |
| spellingShingle | Jiaqi Tang Linglu Qi Yun He Na Li Ze Zhang Xiuwen Zhou Hongyu Su Qiutong Zheng Yumeng Zhang Jianying Tao Zhice Xu In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein Reproductive Biology and Endocrinology Frozen embryo transfer Histamine In vitro fertilization Umbilical vein Vascular tension |
| title | In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein |
| title_full | In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein |
| title_fullStr | In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein |
| title_full_unstemmed | In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein |
| title_short | In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein |
| title_sort | in vitro fertilization with frozen embryo transfer increased histamine mediated contractile sensitivity via pkcβ in human umbilical vein |
| topic | Frozen embryo transfer Histamine In vitro fertilization Umbilical vein Vascular tension |
| url | https://doi.org/10.1186/s12958-023-01103-8 |
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