Results of investigating the characteristics of bone marrow in patients with squamous cell carcinoma of the head and neck, their clinical value
To reveal the specific features of hematopoiesis in patients with head and neck squamous cell carcinoma (HNSCC), the authors analyzed the indicators of 41 bone marrow aspirates in relation to the clinical and morphological characteristics of the tumor and micrometastases detected in bone marrow. Pun...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
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ABV-press
2016-03-01
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| Series: | Опухоли головы и шеи |
| Subjects: | |
| Online Access: | https://ogsh.abvpress.ru/jour/article/view/200 |
| _version_ | 1826558207500222464 |
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| author | E. G. Timonina N. N. Tupitsyn S. O. Podvyaznikov V. A. Spiridonova M. A. Frenkel’ O. P. Kolbatskaya O. A. Chegrinets |
| author_facet | E. G. Timonina N. N. Tupitsyn S. O. Podvyaznikov V. A. Spiridonova M. A. Frenkel’ O. P. Kolbatskaya O. A. Chegrinets |
| author_sort | E. G. Timonina |
| collection | DOAJ |
| description | To reveal the specific features of hematopoiesis in patients with head and neck squamous cell carcinoma (HNSCC), the authors analyzed the indicators of 41 bone marrow aspirates in relation to the clinical and morphological characteristics of the tumor and micrometastases detected in bone marrow. Puncture samples were morphologically and molecularly examined at the Laboratory of Hematopoiesis Immunology, N.N. Blokhin Russian Cancer Research Center. The found changes in the bone marrow cell composition in HNSCC patients showed themselves as an increase in the relative count of segmented neutrophils and a decrease in the level of immature granulocytes with no alternations in the neutrophil maturation index and in the percentage of granulocyte lineage cells. Bone marrow hypocellularity is a characteristic feature of high-grade HNSCC. The significantly enhanced leuko-erythroblastic ratio was due to a reduction in red blood cells: the erythroid cell maturation index rose, by increasing the percentage of bone marrow oxyphilic normoblasts in patients with locally advanced HNSCC. A highly sensitive method for detecting mRNA of the specific squamous cell carcinoma protein E48 was perfected using reverse-transcription polymerase chain reaction and Southern blotting, followed by an enzyme immunoassay that could recognize bone marrow micrometastases in 35 % of the patients with HNSCC. The patients with found bone marrow micrometastases showed a significant decrease in the proportion of polychromatophilic normoblasts and a significant increase in granulocytes in the presence of normal values of lymphocytes and other cellular elements of myelograms. It was established that in the presence of regional lymph node involvement, bone marrow micrometastases were significantly more frequently in the patients with HNSCC and tumor progression over a period of up to 9 months. |
| first_indexed | 2024-04-10T01:59:36Z |
| format | Article |
| id | doaj.art-439755d23aab4a65bb3d3bfd6eb77ffe |
| institution | Directory Open Access Journal |
| issn | 2222-1468 2411-4634 |
| language | Russian |
| last_indexed | 2025-03-14T08:40:49Z |
| publishDate | 2016-03-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Опухоли головы и шеи |
| spelling | doaj.art-439755d23aab4a65bb3d3bfd6eb77ffe2025-03-02T12:53:48ZrusABV-pressОпухоли головы и шеи2222-14682411-46342016-03-0161556710.17650/2222-1468-2016-6-1-55-67189Results of investigating the characteristics of bone marrow in patients with squamous cell carcinoma of the head and neck, their clinical valueE. G. Timonina0N. N. Tupitsyn1S. O. Podvyaznikov2V. A. Spiridonova3M. A. Frenkel’4O. P. Kolbatskaya5O. A. Chegrinets6N.N. Blokhin Russian Cancer Research Center at the Ministry of Health of Russia; 23 Kashirskoe Shosse, Moscow, 115478, Russia;N.N. Blokhin Russian Cancer Research Center at the Ministry of Health of Russia; 23 Kashirskoe Shosse, Moscow, 115478, Russia;Russian Medical Academy of Postgraduate Education at the Ministry of Health of Russia; 2/1 Barrikadnaya St., Moscow, 125993, Russia;A.N. Belozerskiy Research Institute of Physicochemical Biology, M.V. Lomonosov Moscow State University; Build. 40, 1 Leninskie Gory, 119992, Moscow, RussiaN.N. Blokhin Russian Cancer Research Center at the Ministry of Health of Russia; 23 Kashirskoe Shosse, Moscow, 115478, Russia;N.N. Blokhin Russian Cancer Research Center at the Ministry of Health of Russia; 23 Kashirskoe Shosse, Moscow, 115478, Russia;N.N. Blokhin Russian Cancer Research Center at the Ministry of Health of Russia; 23 Kashirskoe Shosse, Moscow, 115478, Russia;To reveal the specific features of hematopoiesis in patients with head and neck squamous cell carcinoma (HNSCC), the authors analyzed the indicators of 41 bone marrow aspirates in relation to the clinical and morphological characteristics of the tumor and micrometastases detected in bone marrow. Puncture samples were morphologically and molecularly examined at the Laboratory of Hematopoiesis Immunology, N.N. Blokhin Russian Cancer Research Center. The found changes in the bone marrow cell composition in HNSCC patients showed themselves as an increase in the relative count of segmented neutrophils and a decrease in the level of immature granulocytes with no alternations in the neutrophil maturation index and in the percentage of granulocyte lineage cells. Bone marrow hypocellularity is a characteristic feature of high-grade HNSCC. The significantly enhanced leuko-erythroblastic ratio was due to a reduction in red blood cells: the erythroid cell maturation index rose, by increasing the percentage of bone marrow oxyphilic normoblasts in patients with locally advanced HNSCC. A highly sensitive method for detecting mRNA of the specific squamous cell carcinoma protein E48 was perfected using reverse-transcription polymerase chain reaction and Southern blotting, followed by an enzyme immunoassay that could recognize bone marrow micrometastases in 35 % of the patients with HNSCC. The patients with found bone marrow micrometastases showed a significant decrease in the proportion of polychromatophilic normoblasts and a significant increase in granulocytes in the presence of normal values of lymphocytes and other cellular elements of myelograms. It was established that in the presence of regional lymph node involvement, bone marrow micrometastases were significantly more frequently in the patients with HNSCC and tumor progression over a period of up to 9 months.https://ogsh.abvpress.ru/jour/article/view/200bone marrowmicrometastaseshead and neck squamous cell carcinomahematopoiesisreverse-transcriptase polymerase chain reactionamplified complementary dna e48 |
| spellingShingle | E. G. Timonina N. N. Tupitsyn S. O. Podvyaznikov V. A. Spiridonova M. A. Frenkel’ O. P. Kolbatskaya O. A. Chegrinets Results of investigating the characteristics of bone marrow in patients with squamous cell carcinoma of the head and neck, their clinical value Опухоли головы и шеи bone marrow micrometastases head and neck squamous cell carcinoma hematopoiesis reverse-transcriptase polymerase chain reaction amplified complementary dna e48 |
| title | Results of investigating the characteristics of bone marrow in patients with squamous cell carcinoma of the head and neck, their clinical value |
| title_full | Results of investigating the characteristics of bone marrow in patients with squamous cell carcinoma of the head and neck, their clinical value |
| title_fullStr | Results of investigating the characteristics of bone marrow in patients with squamous cell carcinoma of the head and neck, their clinical value |
| title_full_unstemmed | Results of investigating the characteristics of bone marrow in patients with squamous cell carcinoma of the head and neck, their clinical value |
| title_short | Results of investigating the characteristics of bone marrow in patients with squamous cell carcinoma of the head and neck, their clinical value |
| title_sort | results of investigating the characteristics of bone marrow in patients with squamous cell carcinoma of the head and neck their clinical value |
| topic | bone marrow micrometastases head and neck squamous cell carcinoma hematopoiesis reverse-transcriptase polymerase chain reaction amplified complementary dna e48 |
| url | https://ogsh.abvpress.ru/jour/article/view/200 |
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