Evaluation by microarray of the potential safety of Sarracenia purpurea L. (Sarraceniaceae) a traditional medicine used by the Cree of Eeyou Istchee
Purpose: The purpose of this study was to assess safety of the traditional antidiabetic extracts of either S. purpurea or its lead active principle, morroniside at the transcriptional level. The overarching objective was to profile and validate transcriptional changes in the cytochrome P450 family o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-10-01
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| Series: | Journal of Pharmacy & Pharmaceutical Sciences |
| Online Access: | https://journals.library.ualberta.ca/jpps/index.php/JPPS/article/view/25231 |
| _version_ | 1827163190100754432 |
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| author | Carolina Cieniak Charlotte McDonald John Nash Asim Muhammad Alaa Badawi Pierre S Haddad Alain Cuerrier Steffany A.L. Bennett Brian C Foster John T Arnason |
| author_facet | Carolina Cieniak Charlotte McDonald John Nash Asim Muhammad Alaa Badawi Pierre S Haddad Alain Cuerrier Steffany A.L. Bennett Brian C Foster John T Arnason |
| author_sort | Carolina Cieniak |
| collection | DOAJ |
| description | Purpose: The purpose of this study was to assess safety of the traditional antidiabetic extracts of either S. purpurea or its lead active principle, morroniside at the transcriptional level. The overarching objective was to profile and validate transcriptional changes in the cytochrome P450 family of genes, in response to treatment with S. purpurea ethanolic extract or its lead active, morroniside. Methods: Transcriptional activity was profiled using a 19K human cDNA microarray in C2BBe1 cells, clone of Caco-2 intestinal cells, which are a model of first-pass metabolism (1, 2). Cells were treated with S. purpurea extract for 4 and 24 hrs, as well as the pure compound morroniside for 4 hrs, to determine their effects. Results: No evidence of cytochrome P450 transcriptome regulation or of transcriptional activation of other diabetes relevant mRNA was detected after rigorous quantitative-PCR validation of microarray results. Conclusion: Our data do not support a transcriptional mechanism of action for either S. purpurea extract or its lead active, morroniside.
This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page. |
| first_indexed | 2024-03-12T04:35:54Z |
| format | Article |
| id | doaj.art-439bd81c39a741189e8b5283f9c2746e |
| institution | Directory Open Access Journal |
| issn | 1482-1826 |
| language | English |
| last_indexed | 2025-03-21T00:56:46Z |
| publishDate | 2015-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmaceutical Sciences |
| spelling | doaj.art-439bd81c39a741189e8b5283f9c2746e2024-08-03T04:48:15ZengFrontiers Media S.A.Journal of Pharmacy & Pharmaceutical Sciences1482-18262015-10-0118410.18433/J3DC74Evaluation by microarray of the potential safety of Sarracenia purpurea L. (Sarraceniaceae) a traditional medicine used by the Cree of Eeyou IstcheeCarolina Cieniak0Charlotte McDonald1John Nash2Asim Muhammad3Alaa Badawi4Pierre S Haddad5Alain Cuerrier6Steffany A.L. Bennett7Brian C Foster8John T Arnason9Biology Department, University of Ottawa, Ottawa, ON, Canada.Biology Department, University of Ottawa, Ottawa, ON, Canada.Office of Biotechnology, Genomics and Population Health, Public Health Agency of Canada, Toronto, ON, Canada.Department of Chemistry, University of Ottawa, Ottawa, ON, Canada.Office of Biotechnology, Genomics and Population Health, Public Health Agency of Canada, Toronto, ON, Canada.Department of Pharmacology, Université de Montréal, Montréal, QC, Canada.Jardin botanique de Montréal, Institut de recherche en biologie végétale, Université de Montréal, Montréal, QC, Canada.Neural Regeneration Laboratory, Ottawa Institute of Systems Biology, Department of Biochemistry Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.Biology Department, University of Ottawa, Ottawa, ON, Canada.Purpose: The purpose of this study was to assess safety of the traditional antidiabetic extracts of either S. purpurea or its lead active principle, morroniside at the transcriptional level. The overarching objective was to profile and validate transcriptional changes in the cytochrome P450 family of genes, in response to treatment with S. purpurea ethanolic extract or its lead active, morroniside. Methods: Transcriptional activity was profiled using a 19K human cDNA microarray in C2BBe1 cells, clone of Caco-2 intestinal cells, which are a model of first-pass metabolism (1, 2). Cells were treated with S. purpurea extract for 4 and 24 hrs, as well as the pure compound morroniside for 4 hrs, to determine their effects. Results: No evidence of cytochrome P450 transcriptome regulation or of transcriptional activation of other diabetes relevant mRNA was detected after rigorous quantitative-PCR validation of microarray results. Conclusion: Our data do not support a transcriptional mechanism of action for either S. purpurea extract or its lead active, morroniside. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.https://journals.library.ualberta.ca/jpps/index.php/JPPS/article/view/25231 |
| spellingShingle | Carolina Cieniak Charlotte McDonald John Nash Asim Muhammad Alaa Badawi Pierre S Haddad Alain Cuerrier Steffany A.L. Bennett Brian C Foster John T Arnason Evaluation by microarray of the potential safety of Sarracenia purpurea L. (Sarraceniaceae) a traditional medicine used by the Cree of Eeyou Istchee Journal of Pharmacy & Pharmaceutical Sciences |
| title | Evaluation by microarray of the potential safety of Sarracenia purpurea L. (Sarraceniaceae) a traditional medicine used by the Cree of Eeyou Istchee |
| title_full | Evaluation by microarray of the potential safety of Sarracenia purpurea L. (Sarraceniaceae) a traditional medicine used by the Cree of Eeyou Istchee |
| title_fullStr | Evaluation by microarray of the potential safety of Sarracenia purpurea L. (Sarraceniaceae) a traditional medicine used by the Cree of Eeyou Istchee |
| title_full_unstemmed | Evaluation by microarray of the potential safety of Sarracenia purpurea L. (Sarraceniaceae) a traditional medicine used by the Cree of Eeyou Istchee |
| title_short | Evaluation by microarray of the potential safety of Sarracenia purpurea L. (Sarraceniaceae) a traditional medicine used by the Cree of Eeyou Istchee |
| title_sort | evaluation by microarray of the potential safety of sarracenia purpurea l sarraceniaceae a traditional medicine used by the cree of eeyou istchee |
| url | https://journals.library.ualberta.ca/jpps/index.php/JPPS/article/view/25231 |
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