Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning.
Phosphoglucomutase (PGM) catalyses the interconversion of glucose 1-phosphate (G1P) and glucose 6-phosphate (G6P) and exists as plastidial (pPGM) and cytosolic (cPGM) isoforms. The plastidial isoform is essential for transitory starch synthesis in chloroplasts of leaves, whereas the cytosolic counte...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0112468 |
| _version_ | 1818399984131768320 |
|---|---|
| author | Irina Malinova Hans-Henning Kunz Saleh Alseekh Karoline Herbst Alisdair R Fernie Markus Gierth Joerg Fettke |
| author_facet | Irina Malinova Hans-Henning Kunz Saleh Alseekh Karoline Herbst Alisdair R Fernie Markus Gierth Joerg Fettke |
| author_sort | Irina Malinova |
| collection | DOAJ |
| description | Phosphoglucomutase (PGM) catalyses the interconversion of glucose 1-phosphate (G1P) and glucose 6-phosphate (G6P) and exists as plastidial (pPGM) and cytosolic (cPGM) isoforms. The plastidial isoform is essential for transitory starch synthesis in chloroplasts of leaves, whereas the cytosolic counterpart is essential for glucose phosphate partitioning and, therefore, for syntheses of sucrose and cell wall components. In Arabidopsis two cytosolic isoforms (PGM2 and PGM3) exist. Both PGM2 and PGM3 are redundant in function as single mutants reveal only small or no alterations compared to wild type with respect to plant primary metabolism. So far, there are no reports of Arabidopsis plants lacking the entire cPGM or total PGM activity, respectively. Therefore, amiRNA transgenic plants were generated and used for analyses of various parameters such as growth, development, and starch metabolism. The lack of the entire cPGM activity resulted in a strongly reduced growth revealed by decreased rosette fresh weight, shorter roots, and reduced seed production compared to wild type. By contrast content of starch, sucrose, maltose and cell wall components were significantly increased. The lack of both cPGM and pPGM activities in Arabidopsis resulted in dwarf growth, prematurely die off, and inability to develop a functional inflorescence. The combined results are discussed in comparison to potato, the only described mutant with lack of total PGM activity. |
| first_indexed | 2024-12-14T07:29:21Z |
| format | Article |
| id | doaj.art-439e8ce0ebba414d9320260f924bd473 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T07:29:21Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-439e8ce0ebba414d9320260f924bd4732022-12-21T23:11:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11246810.1371/journal.pone.0112468Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning.Irina MalinovaHans-Henning KunzSaleh AlseekhKaroline HerbstAlisdair R FernieMarkus GierthJoerg FettkePhosphoglucomutase (PGM) catalyses the interconversion of glucose 1-phosphate (G1P) and glucose 6-phosphate (G6P) and exists as plastidial (pPGM) and cytosolic (cPGM) isoforms. The plastidial isoform is essential for transitory starch synthesis in chloroplasts of leaves, whereas the cytosolic counterpart is essential for glucose phosphate partitioning and, therefore, for syntheses of sucrose and cell wall components. In Arabidopsis two cytosolic isoforms (PGM2 and PGM3) exist. Both PGM2 and PGM3 are redundant in function as single mutants reveal only small or no alterations compared to wild type with respect to plant primary metabolism. So far, there are no reports of Arabidopsis plants lacking the entire cPGM or total PGM activity, respectively. Therefore, amiRNA transgenic plants were generated and used for analyses of various parameters such as growth, development, and starch metabolism. The lack of the entire cPGM activity resulted in a strongly reduced growth revealed by decreased rosette fresh weight, shorter roots, and reduced seed production compared to wild type. By contrast content of starch, sucrose, maltose and cell wall components were significantly increased. The lack of both cPGM and pPGM activities in Arabidopsis resulted in dwarf growth, prematurely die off, and inability to develop a functional inflorescence. The combined results are discussed in comparison to potato, the only described mutant with lack of total PGM activity.https://doi.org/10.1371/journal.pone.0112468 |
| spellingShingle | Irina Malinova Hans-Henning Kunz Saleh Alseekh Karoline Herbst Alisdair R Fernie Markus Gierth Joerg Fettke Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning. PLoS ONE |
| title | Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning. |
| title_full | Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning. |
| title_fullStr | Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning. |
| title_full_unstemmed | Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning. |
| title_short | Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning. |
| title_sort | reduction of the cytosolic phosphoglucomutase in arabidopsis reveals impact on plant growth seed and root development and carbohydrate partitioning |
| url | https://doi.org/10.1371/journal.pone.0112468 |
| work_keys_str_mv | AT irinamalinova reductionofthecytosolicphosphoglucomutaseinarabidopsisrevealsimpactonplantgrowthseedandrootdevelopmentandcarbohydratepartitioning AT hanshenningkunz reductionofthecytosolicphosphoglucomutaseinarabidopsisrevealsimpactonplantgrowthseedandrootdevelopmentandcarbohydratepartitioning AT salehalseekh reductionofthecytosolicphosphoglucomutaseinarabidopsisrevealsimpactonplantgrowthseedandrootdevelopmentandcarbohydratepartitioning AT karolineherbst reductionofthecytosolicphosphoglucomutaseinarabidopsisrevealsimpactonplantgrowthseedandrootdevelopmentandcarbohydratepartitioning AT alisdairrfernie reductionofthecytosolicphosphoglucomutaseinarabidopsisrevealsimpactonplantgrowthseedandrootdevelopmentandcarbohydratepartitioning AT markusgierth reductionofthecytosolicphosphoglucomutaseinarabidopsisrevealsimpactonplantgrowthseedandrootdevelopmentandcarbohydratepartitioning AT joergfettke reductionofthecytosolicphosphoglucomutaseinarabidopsisrevealsimpactonplantgrowthseedandrootdevelopmentandcarbohydratepartitioning |