An Inducible System for Rapid Degradation of Specific Cellular Proteins Using Proteasome Adaptors.
A common way to study protein function is to deplete the protein of interest from cells and observe the response. Traditional methods involve disrupting gene expression but these techniques are only effective against newly synthesized proteins and leave previously existing and stable proteins untouc...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4820223?pdf=render |
| _version_ | 1818420134583205888 |
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| author | Shameika R Wilmington Andreas Matouschek |
| author_facet | Shameika R Wilmington Andreas Matouschek |
| author_sort | Shameika R Wilmington |
| collection | DOAJ |
| description | A common way to study protein function is to deplete the protein of interest from cells and observe the response. Traditional methods involve disrupting gene expression but these techniques are only effective against newly synthesized proteins and leave previously existing and stable proteins untouched. Here, we introduce a technique that induces the rapid degradation of specific proteins in mammalian cells by shuttling the proteins to the proteasome for degradation in a ubiquitin-independent manner. We present two implementations of the system in human culture cells that can be used individually to control protein concentration. Our study presents a simple, robust, and flexible technology platform for manipulating intracellular protein levels. |
| first_indexed | 2024-12-14T12:49:38Z |
| format | Article |
| id | doaj.art-43a242db37284ef3bafe04486f59e5c6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T12:49:38Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-43a242db37284ef3bafe04486f59e5c62022-12-21T23:00:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01114e015267910.1371/journal.pone.0152679An Inducible System for Rapid Degradation of Specific Cellular Proteins Using Proteasome Adaptors.Shameika R WilmingtonAndreas MatouschekA common way to study protein function is to deplete the protein of interest from cells and observe the response. Traditional methods involve disrupting gene expression but these techniques are only effective against newly synthesized proteins and leave previously existing and stable proteins untouched. Here, we introduce a technique that induces the rapid degradation of specific proteins in mammalian cells by shuttling the proteins to the proteasome for degradation in a ubiquitin-independent manner. We present two implementations of the system in human culture cells that can be used individually to control protein concentration. Our study presents a simple, robust, and flexible technology platform for manipulating intracellular protein levels.http://europepmc.org/articles/PMC4820223?pdf=render |
| spellingShingle | Shameika R Wilmington Andreas Matouschek An Inducible System for Rapid Degradation of Specific Cellular Proteins Using Proteasome Adaptors. PLoS ONE |
| title | An Inducible System for Rapid Degradation of Specific Cellular Proteins Using Proteasome Adaptors. |
| title_full | An Inducible System for Rapid Degradation of Specific Cellular Proteins Using Proteasome Adaptors. |
| title_fullStr | An Inducible System for Rapid Degradation of Specific Cellular Proteins Using Proteasome Adaptors. |
| title_full_unstemmed | An Inducible System for Rapid Degradation of Specific Cellular Proteins Using Proteasome Adaptors. |
| title_short | An Inducible System for Rapid Degradation of Specific Cellular Proteins Using Proteasome Adaptors. |
| title_sort | inducible system for rapid degradation of specific cellular proteins using proteasome adaptors |
| url | http://europepmc.org/articles/PMC4820223?pdf=render |
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