Designing a new alginate-fibrinogen biomaterial composite hydrogel for wound healing
Abstract Wound healing is a complex process and rapid healing necessitates a proper micro-environment. Therefore, design and fabrication of an efficacious wound dressing is an impressive innovation in the field of wound healing. The fabricated wound dressing in this scenario was designed using a com...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-11282-w |
| _version_ | 1811304773873303552 |
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| author | Marjan Soleimanpour Samaneh Sadat Mirhaji Samira Jafari Hossein Derakhshankhah Fatemeh Mamashli Hadi Nedaei Mohammad Reza Karimi Hamidreza Motasadizadeh Yousef Fatahi Atiyeh Ghasemi Maryam Sadat Nezamtaheri Mohadese Khajezade Masoumeh Teimouri Bahram Goliaei Cédric Delattre Ali Akbar Saboury |
| author_facet | Marjan Soleimanpour Samaneh Sadat Mirhaji Samira Jafari Hossein Derakhshankhah Fatemeh Mamashli Hadi Nedaei Mohammad Reza Karimi Hamidreza Motasadizadeh Yousef Fatahi Atiyeh Ghasemi Maryam Sadat Nezamtaheri Mohadese Khajezade Masoumeh Teimouri Bahram Goliaei Cédric Delattre Ali Akbar Saboury |
| author_sort | Marjan Soleimanpour |
| collection | DOAJ |
| description | Abstract Wound healing is a complex process and rapid healing necessitates a proper micro-environment. Therefore, design and fabrication of an efficacious wound dressing is an impressive innovation in the field of wound healing. The fabricated wound dressing in this scenario was designed using a combination of the appropriate coagulating and anti-bacterial materials like fibrinogen (as coagulating agent), nisin (as anti-bacterial agent), ethylenediaminetetraacetic acid (as anti-bacterial agent), and alginate (as wound healing agent). Biophysical characterization showed that the interaction of fibrinogen and alginate was associated with minor changes in the secondary structure of the protein. Conformational studies showed that the protein was structurally stable at 42 °C, is the maximum temperature of the infected wound. The properties of the hydrogel such as swelling, mechanical resistance, nisin release, antibacterial activity, cytotoxicity, gel porosity, and blood coagulation were assessed. The results showed a slow release for the nisin during 48 h. Antibacterial studies showed an inhibitory effect on the growth of Gram-negative and Gram-positive bacteria. The hydrogel was also capable to absorb a considerable amount of water and provide oxygenation as well as incorporation of the drug into its structure due to its sufficient porosity. Scanning electron microscopy showed pore sizes of about 14–198 µm in the hydrogel. Cell viability studies indicated high biocompatibility of the hydrogel. Blood coagulation test also confirmed the effectiveness of the synthesized hydrogel in accelerating the process of blood clot formation. In vivo studies showed higher rates of wound healing, re-epithelialization, and collagen deposition. According to the findings from in vitro as well as in vivo studies, the designed hydrogel can be considered as a novel attractive wound dressing after further prerequisite assessments. |
| first_indexed | 2024-04-13T08:13:29Z |
| format | Article |
| id | doaj.art-43a31a5f227c4f3daf031e7bc1370075 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T08:13:29Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-43a31a5f227c4f3daf031e7bc13700752022-12-22T02:54:53ZengNature PortfolioScientific Reports2045-23222022-05-0112111710.1038/s41598-022-11282-wDesigning a new alginate-fibrinogen biomaterial composite hydrogel for wound healingMarjan Soleimanpour0Samaneh Sadat Mirhaji1Samira Jafari2Hossein Derakhshankhah3Fatemeh Mamashli4Hadi Nedaei5Mohammad Reza Karimi6Hamidreza Motasadizadeh7Yousef Fatahi8Atiyeh Ghasemi9Maryam Sadat Nezamtaheri10Mohadese Khajezade11Masoumeh Teimouri12Bahram Goliaei13Cédric Delattre14Ali Akbar Saboury15Institute of Biochemistry and Biophysics, University of TehranInstitute of Biochemistry and Biophysics, University of TehranPharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical SciencesPharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical SciencesInstitute of Biochemistry and Biophysics, University of TehranInstitute of Biochemistry and Biophysics, University of TehranPolymer Laboratory, School of Chemistry, College of Science, University of TehranDepartment of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical SciencesDepartment of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical SciencesInstitute of Biochemistry and Biophysics, University of TehranInstitute of Biochemistry and Biophysics, University of TehranDepartment of Petroleum Microbiology, Academic Center for Education, Culture and Research (ACECR), Shahid Beheshti UniversityFaculty of New Sciences and Technologies, University of TehranInstitute of Biochemistry and Biophysics, University of TehranInstitut Universitaire de France (IUF)Institute of Biochemistry and Biophysics, University of TehranAbstract Wound healing is a complex process and rapid healing necessitates a proper micro-environment. Therefore, design and fabrication of an efficacious wound dressing is an impressive innovation in the field of wound healing. The fabricated wound dressing in this scenario was designed using a combination of the appropriate coagulating and anti-bacterial materials like fibrinogen (as coagulating agent), nisin (as anti-bacterial agent), ethylenediaminetetraacetic acid (as anti-bacterial agent), and alginate (as wound healing agent). Biophysical characterization showed that the interaction of fibrinogen and alginate was associated with minor changes in the secondary structure of the protein. Conformational studies showed that the protein was structurally stable at 42 °C, is the maximum temperature of the infected wound. The properties of the hydrogel such as swelling, mechanical resistance, nisin release, antibacterial activity, cytotoxicity, gel porosity, and blood coagulation were assessed. The results showed a slow release for the nisin during 48 h. Antibacterial studies showed an inhibitory effect on the growth of Gram-negative and Gram-positive bacteria. The hydrogel was also capable to absorb a considerable amount of water and provide oxygenation as well as incorporation of the drug into its structure due to its sufficient porosity. Scanning electron microscopy showed pore sizes of about 14–198 µm in the hydrogel. Cell viability studies indicated high biocompatibility of the hydrogel. Blood coagulation test also confirmed the effectiveness of the synthesized hydrogel in accelerating the process of blood clot formation. In vivo studies showed higher rates of wound healing, re-epithelialization, and collagen deposition. According to the findings from in vitro as well as in vivo studies, the designed hydrogel can be considered as a novel attractive wound dressing after further prerequisite assessments.https://doi.org/10.1038/s41598-022-11282-w |
| spellingShingle | Marjan Soleimanpour Samaneh Sadat Mirhaji Samira Jafari Hossein Derakhshankhah Fatemeh Mamashli Hadi Nedaei Mohammad Reza Karimi Hamidreza Motasadizadeh Yousef Fatahi Atiyeh Ghasemi Maryam Sadat Nezamtaheri Mohadese Khajezade Masoumeh Teimouri Bahram Goliaei Cédric Delattre Ali Akbar Saboury Designing a new alginate-fibrinogen biomaterial composite hydrogel for wound healing Scientific Reports |
| title | Designing a new alginate-fibrinogen biomaterial composite hydrogel for wound healing |
| title_full | Designing a new alginate-fibrinogen biomaterial composite hydrogel for wound healing |
| title_fullStr | Designing a new alginate-fibrinogen biomaterial composite hydrogel for wound healing |
| title_full_unstemmed | Designing a new alginate-fibrinogen biomaterial composite hydrogel for wound healing |
| title_short | Designing a new alginate-fibrinogen biomaterial composite hydrogel for wound healing |
| title_sort | designing a new alginate fibrinogen biomaterial composite hydrogel for wound healing |
| url | https://doi.org/10.1038/s41598-022-11282-w |
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