Fungal flora associated with internal symptoms of grapevine decline in Kerman province
To study of fungi and symptoms associated with vine trunk diseases, various vineyards in Kerman province were inspected during spring and summer of 2012. Samples showing decline symptoms were collected from trunk and branches of vines. Fungal isolation from affected tissues were done on malt extract...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Shahid Bahonar University of Kerman
2014-08-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_1316_70451abf497270a36e5ef5bf4bfe65c9.pdf |
| _version_ | 1818302212998168576 |
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| author | Maryam Arab Nezhad Hamid Mohammadi Hossein Masoumi Homayoun Farahmand |
| author_facet | Maryam Arab Nezhad Hamid Mohammadi Hossein Masoumi Homayoun Farahmand |
| author_sort | Maryam Arab Nezhad |
| collection | DOAJ |
| description | To study of fungi and symptoms associated with vine trunk diseases, various vineyards in Kerman province were inspected during spring and summer of 2012. Samples showing decline symptoms were collected from trunk and branches of vines. Fungal isolation from affected tissues were done on malt extract agar medium (MEA) supplemented with 1 g l<sup>–1</sup> streptomycin sulphate (MEAS). A total of 476 fungal isolates from diseased grapevines were obtained. Fungal isolates were identified based on their morphological and molecular characteristics. Morphological identifications of <em>Phaeoacremonium </em>spp. were confirmed by sequence analysis of partial β-tubulin gene (BT) sequences amplification using primers T1 and Bt2b. <em>Botryosphaeria</em> isolates were also confirmed by amplification and sequence of the internal transcribed spacer (ITS) region using the primers ITS4 and ITS5. The most common fungi isolated from vineyards were <em>Phaeoacremonium aleophilum</em> and <em>Phaeomoniella chlamydospora</em> with 103 and 30 isolates, respectively. This is the first report of molecular identification of <em>Pm.</em> <em>aleophilum</em>, <em>Pa. chlamydospora</em>, <em>Pm. parasiticum</em> and <em>Botryosphaeria dothidea</em> associated with different internal symptoms of vine decline in Kerman province. |
| first_indexed | 2024-12-13T05:35:19Z |
| format | Article |
| id | doaj.art-43a367480894420cba83e1964f53f413 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-12-13T05:35:19Z |
| publishDate | 2014-08-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-43a367480894420cba83e1964f53f4132022-12-21T23:57:56ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002014-08-016211513210.22103/jab.2014.13161316Fungal flora associated with internal symptoms of grapevine decline in Kerman provinceMaryam Arab NezhadHamid MohammadiHossein MasoumiHomayoun FarahmandTo study of fungi and symptoms associated with vine trunk diseases, various vineyards in Kerman province were inspected during spring and summer of 2012. Samples showing decline symptoms were collected from trunk and branches of vines. Fungal isolation from affected tissues were done on malt extract agar medium (MEA) supplemented with 1 g l<sup>–1</sup> streptomycin sulphate (MEAS). A total of 476 fungal isolates from diseased grapevines were obtained. Fungal isolates were identified based on their morphological and molecular characteristics. Morphological identifications of <em>Phaeoacremonium </em>spp. were confirmed by sequence analysis of partial β-tubulin gene (BT) sequences amplification using primers T1 and Bt2b. <em>Botryosphaeria</em> isolates were also confirmed by amplification and sequence of the internal transcribed spacer (ITS) region using the primers ITS4 and ITS5. The most common fungi isolated from vineyards were <em>Phaeoacremonium aleophilum</em> and <em>Phaeomoniella chlamydospora</em> with 103 and 30 isolates, respectively. This is the first report of molecular identification of <em>Pm.</em> <em>aleophilum</em>, <em>Pa. chlamydospora</em>, <em>Pm. parasiticum</em> and <em>Botryosphaeria dothidea</em> associated with different internal symptoms of vine decline in Kerman province.https://jab.uk.ac.ir/article_1316_70451abf497270a36e5ef5bf4bfe65c9.pdfbeta tubulinitsvitis vinifera |
| spellingShingle | Maryam Arab Nezhad Hamid Mohammadi Hossein Masoumi Homayoun Farahmand Fungal flora associated with internal symptoms of grapevine decline in Kerman province مجله بیوتکنولوژی کشاورزی beta tubulin its vitis vinifera |
| title | Fungal flora associated with internal symptoms of grapevine decline in Kerman province |
| title_full | Fungal flora associated with internal symptoms of grapevine decline in Kerman province |
| title_fullStr | Fungal flora associated with internal symptoms of grapevine decline in Kerman province |
| title_full_unstemmed | Fungal flora associated with internal symptoms of grapevine decline in Kerman province |
| title_short | Fungal flora associated with internal symptoms of grapevine decline in Kerman province |
| title_sort | fungal flora associated with internal symptoms of grapevine decline in kerman province |
| topic | beta tubulin its vitis vinifera |
| url | https://jab.uk.ac.ir/article_1316_70451abf497270a36e5ef5bf4bfe65c9.pdf |
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