Single-polyp metabolomics for coral health assessment
Abstract Coral reef ecosystems supported by environmentally sensitive reef-building corals face serious threats from human activities. Our understanding of these reef threats is hampered by the lack of sufficiently sensitive coral environmental impact assessment systems. In this study, we establishe...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-53294-8 |
| _version_ | 1797275134911840256 |
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| author | Akira Iguchi Mariko Iijima Nanami Mizusawa Yoshikazu Ohno Ko Yasumoto Atsushi Suzuki Shunichi Suga Ken Tanaka Kei Zaitsu |
| author_facet | Akira Iguchi Mariko Iijima Nanami Mizusawa Yoshikazu Ohno Ko Yasumoto Atsushi Suzuki Shunichi Suga Ken Tanaka Kei Zaitsu |
| author_sort | Akira Iguchi |
| collection | DOAJ |
| description | Abstract Coral reef ecosystems supported by environmentally sensitive reef-building corals face serious threats from human activities. Our understanding of these reef threats is hampered by the lack of sufficiently sensitive coral environmental impact assessment systems. In this study, we established a platform for metabolomic analysis at the single-coral-polyp level using state-of-the-art mass spectrometry (probe electrospray ionization/tandem mass spectrometry; PESI/MS/MS) capable of fine-scale analysis. We analyzed the impact of the organic UV filter, benzophenone (BP), which has a negative impact on corals. We also analyzed ammonium and nitrate samples, which affect the environmental sensitivity of coral-zooxanthella (Symbiodiniaceae) holobionts, to provide new insights into coral biology with a focus on metabolites. The method established in this study breaks new ground by combining PESI/MS/MS with a technique for coral polyps that can control the presence or absence of zooxanthellae in corals, enabling functions of zooxanthellae to be assessed on a polyp-by-polyp basis for the first time. This system will clarify biological mechanisms of corals and will become an important model system for environmental impact assessment using marine organisms. |
| first_indexed | 2024-03-07T15:08:58Z |
| format | Article |
| id | doaj.art-43a379318a62454796c203dd6093d005 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:08:58Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-43a379318a62454796c203dd6093d0052024-03-05T18:44:54ZengNature PortfolioScientific Reports2045-23222024-03-011411710.1038/s41598-024-53294-8Single-polyp metabolomics for coral health assessmentAkira Iguchi0Mariko Iijima1Nanami Mizusawa2Yoshikazu Ohno3Ko Yasumoto4Atsushi Suzuki5Shunichi Suga6Ken Tanaka7Kei Zaitsu8Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST)Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST)School of Marine Biosciences, Kitasato UniversitySchool of Marine Biosciences, Kitasato UniversitySchool of Marine Biosciences, Kitasato UniversityGeological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST)Research Laboratories, KOSÉ CorporationResearch Laboratories, KOSÉ CorporationMultimodal Informatics and Wide-Data Analytics Laboratory (MiWA-Lab.), Faculty of Biology-Oriented Science and Technology, Kindai UniversityAbstract Coral reef ecosystems supported by environmentally sensitive reef-building corals face serious threats from human activities. Our understanding of these reef threats is hampered by the lack of sufficiently sensitive coral environmental impact assessment systems. In this study, we established a platform for metabolomic analysis at the single-coral-polyp level using state-of-the-art mass spectrometry (probe electrospray ionization/tandem mass spectrometry; PESI/MS/MS) capable of fine-scale analysis. We analyzed the impact of the organic UV filter, benzophenone (BP), which has a negative impact on corals. We also analyzed ammonium and nitrate samples, which affect the environmental sensitivity of coral-zooxanthella (Symbiodiniaceae) holobionts, to provide new insights into coral biology with a focus on metabolites. The method established in this study breaks new ground by combining PESI/MS/MS with a technique for coral polyps that can control the presence or absence of zooxanthellae in corals, enabling functions of zooxanthellae to be assessed on a polyp-by-polyp basis for the first time. This system will clarify biological mechanisms of corals and will become an important model system for environmental impact assessment using marine organisms.https://doi.org/10.1038/s41598-024-53294-8 |
| spellingShingle | Akira Iguchi Mariko Iijima Nanami Mizusawa Yoshikazu Ohno Ko Yasumoto Atsushi Suzuki Shunichi Suga Ken Tanaka Kei Zaitsu Single-polyp metabolomics for coral health assessment Scientific Reports |
| title | Single-polyp metabolomics for coral health assessment |
| title_full | Single-polyp metabolomics for coral health assessment |
| title_fullStr | Single-polyp metabolomics for coral health assessment |
| title_full_unstemmed | Single-polyp metabolomics for coral health assessment |
| title_short | Single-polyp metabolomics for coral health assessment |
| title_sort | single polyp metabolomics for coral health assessment |
| url | https://doi.org/10.1038/s41598-024-53294-8 |
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