Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila
Our understanding of the molecular mechanisms underlying sleep homeostasis is limited. We have taken a systematic approach to study neural signaling by the transmitter 5-hydroxytryptamine (5-HT) in drosophila. We have generated knockout and knockin lines for Trh, the 5-HT synthesizing enzyme and all...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-10-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/26519 |
| _version_ | 1818019544408522752 |
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| author | Yongjun Qian Yue Cao Bowen Deng Guang Yang Jiayun Li Rui Xu Dandan zhang Juan Huang Yi Rao |
| author_facet | Yongjun Qian Yue Cao Bowen Deng Guang Yang Jiayun Li Rui Xu Dandan zhang Juan Huang Yi Rao |
| author_sort | Yongjun Qian |
| collection | DOAJ |
| description | Our understanding of the molecular mechanisms underlying sleep homeostasis is limited. We have taken a systematic approach to study neural signaling by the transmitter 5-hydroxytryptamine (5-HT) in drosophila. We have generated knockout and knockin lines for Trh, the 5-HT synthesizing enzyme and all five 5-HT receptors, making it possible for us to determine their expression patterns and to investigate their functional roles. Loss of the Trh, 5HT1a or 5HT2b gene decreased sleep time whereas loss of the Trh or 5HT2b gene diminished sleep rebound after sleep deprivation. 5HT2b expression in a small subset of, probably a single pair of, neurons in the dorsal fan-shaped body (dFB) is functionally essential: elimination of the 5HT2b gene from these neurons led to loss of sleep homeostasis. Genetic ablation of 5HT2b neurons in the dFB decreased sleep and impaired sleep homeostasis. Our results have shown that serotonergic signaling in specific neurons is required for the regulation of sleep homeostasis. |
| first_indexed | 2024-04-14T07:52:34Z |
| format | Article |
| id | doaj.art-43a46f836b8f4c90abfe76113d279232 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:52:34Z |
| publishDate | 2017-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-43a46f836b8f4c90abfe76113d2792322022-12-22T02:05:08ZengeLife Sciences Publications LtdeLife2050-084X2017-10-01610.7554/eLife.26519Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophilaYongjun Qian0https://orcid.org/0000-0002-2696-6730Yue Cao1Bowen Deng2Guang Yang3Jiayun Li4Rui Xu5Dandan zhang6Juan Huang7Yi Rao8https://orcid.org/0000-0002-0405-5426Peking-Tsinghua Center for Life Sciences, State Key Laboratory of Biomembrane and Membrane Biology, PKU-IDG/McGovern Institute For Brain Research, Beijing Advanced Innovation Center for Genomics, School of Life Sciences, Peking University, Beijing, China; National Institute of Biological Sciences, Beijing, ChinaPeking-Tsinghua Center for Life Sciences, State Key Laboratory of Biomembrane and Membrane Biology, PKU-IDG/McGovern Institute For Brain Research, Beijing Advanced Innovation Center for Genomics, School of Life Sciences, Peking University, Beijing, ChinaPeking-Tsinghua Center for Life Sciences, State Key Laboratory of Biomembrane and Membrane Biology, PKU-IDG/McGovern Institute For Brain Research, Beijing Advanced Innovation Center for Genomics, School of Life Sciences, Peking University, Beijing, China; National Institute of Biological Sciences, Beijing, ChinaPeking-Tsinghua Center for Life Sciences, State Key Laboratory of Biomembrane and Membrane Biology, PKU-IDG/McGovern Institute For Brain Research, Beijing Advanced Innovation Center for Genomics, School of Life Sciences, Peking University, Beijing, ChinaPeking-Tsinghua Center for Life Sciences, State Key Laboratory of Biomembrane and Membrane Biology, PKU-IDG/McGovern Institute For Brain Research, Beijing Advanced Innovation Center for Genomics, School of Life Sciences, Peking University, Beijing, ChinaSchool of Basic Medical Sciences, Nanjing Medical University, Nanjing, ChinaSchool of Basic Medical Sciences, Nanjing Medical University, Nanjing, ChinaSchool of Basic Medical Sciences, Nanjing Medical University, Nanjing, ChinaPeking-Tsinghua Center for Life Sciences, State Key Laboratory of Biomembrane and Membrane Biology, PKU-IDG/McGovern Institute For Brain Research, Beijing Advanced Innovation Center for Genomics, School of Life Sciences, Peking University, Beijing, China; National Institute of Biological Sciences, Beijing, ChinaOur understanding of the molecular mechanisms underlying sleep homeostasis is limited. We have taken a systematic approach to study neural signaling by the transmitter 5-hydroxytryptamine (5-HT) in drosophila. We have generated knockout and knockin lines for Trh, the 5-HT synthesizing enzyme and all five 5-HT receptors, making it possible for us to determine their expression patterns and to investigate their functional roles. Loss of the Trh, 5HT1a or 5HT2b gene decreased sleep time whereas loss of the Trh or 5HT2b gene diminished sleep rebound after sleep deprivation. 5HT2b expression in a small subset of, probably a single pair of, neurons in the dorsal fan-shaped body (dFB) is functionally essential: elimination of the 5HT2b gene from these neurons led to loss of sleep homeostasis. Genetic ablation of 5HT2b neurons in the dFB decreased sleep and impaired sleep homeostasis. Our results have shown that serotonergic signaling in specific neurons is required for the regulation of sleep homeostasis.https://elifesciences.org/articles/26519sleep homeostasisserotonin5HT2b receptordFB neurons |
| spellingShingle | Yongjun Qian Yue Cao Bowen Deng Guang Yang Jiayun Li Rui Xu Dandan zhang Juan Huang Yi Rao Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila eLife sleep homeostasis serotonin 5HT2b receptor dFB neurons |
| title | Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila |
| title_full | Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila |
| title_fullStr | Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila |
| title_full_unstemmed | Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila |
| title_short | Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila |
| title_sort | sleep homeostasis regulated by 5ht2b receptor in a small subset of neurons in the dorsal fan shaped body of drosophila |
| topic | sleep homeostasis serotonin 5HT2b receptor dFB neurons |
| url | https://elifesciences.org/articles/26519 |
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