Prefabricated platinum nanomaterial matrix for MALDI-MS imaging of oligosaccharides and lipids in plant tissues
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) can visualize the spatial distribution characteristics of molecules in tissues in situ, in which the matrix plays a key role. In this paper, we propose a platinum nanomaterial pre-coated matrix, which can be prepared i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1105374/full |
| _version_ | 1797950646410805248 |
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| author | Yu-Lin Shen Si-Jia Zhuang Fan Yang Can Gong Xu Xu |
| author_facet | Yu-Lin Shen Si-Jia Zhuang Fan Yang Can Gong Xu Xu |
| author_sort | Yu-Lin Shen |
| collection | DOAJ |
| description | Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) can visualize the spatial distribution characteristics of molecules in tissues in situ, in which the matrix plays a key role. In this paper, we propose a platinum nanomaterial pre-coated matrix, which can be prepared in bulk by sputtering platinum nanoparticles onto slides using an ion sputterer and then used for MALDI-MS analysis by placing tissue sections on the matrix. We used this matrix for MALDI-MS imaging analysis of corn kernels and germinated wheat sections, and the results show that triacylglycerides were mainly distributed in the embryo of corn kernels and germinated wheat, and sugars were mainly distributed in the endosperm, with the highest content of disaccharides.It provides a simple and reliable experimental condition for analyzing the distribution of oligosaccharide and lipid components in plant tissues. |
| first_indexed | 2024-04-10T22:18:26Z |
| format | Article |
| id | doaj.art-43a81eeb9046488a92ef901968897cf5 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T22:18:26Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-43a81eeb9046488a92ef901968897cf52023-01-18T05:33:38ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011410.3389/fpls.2023.11053741105374Prefabricated platinum nanomaterial matrix for MALDI-MS imaging of oligosaccharides and lipids in plant tissuesYu-Lin ShenSi-Jia ZhuangFan YangCan GongXu XuMatrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) can visualize the spatial distribution characteristics of molecules in tissues in situ, in which the matrix plays a key role. In this paper, we propose a platinum nanomaterial pre-coated matrix, which can be prepared in bulk by sputtering platinum nanoparticles onto slides using an ion sputterer and then used for MALDI-MS analysis by placing tissue sections on the matrix. We used this matrix for MALDI-MS imaging analysis of corn kernels and germinated wheat sections, and the results show that triacylglycerides were mainly distributed in the embryo of corn kernels and germinated wheat, and sugars were mainly distributed in the endosperm, with the highest content of disaccharides.It provides a simple and reliable experimental condition for analyzing the distribution of oligosaccharide and lipid components in plant tissues.https://www.frontiersin.org/articles/10.3389/fpls.2023.1105374/fullmatrix-assisted laser desorption ionization mass spectrometry imagingmatrixplatinum nanomaterialsplant tissueoligosaccharidelipid |
| spellingShingle | Yu-Lin Shen Si-Jia Zhuang Fan Yang Can Gong Xu Xu Prefabricated platinum nanomaterial matrix for MALDI-MS imaging of oligosaccharides and lipids in plant tissues Frontiers in Plant Science matrix-assisted laser desorption ionization mass spectrometry imaging matrix platinum nanomaterials plant tissue oligosaccharide lipid |
| title | Prefabricated platinum nanomaterial matrix for MALDI-MS imaging of oligosaccharides and lipids in plant tissues |
| title_full | Prefabricated platinum nanomaterial matrix for MALDI-MS imaging of oligosaccharides and lipids in plant tissues |
| title_fullStr | Prefabricated platinum nanomaterial matrix for MALDI-MS imaging of oligosaccharides and lipids in plant tissues |
| title_full_unstemmed | Prefabricated platinum nanomaterial matrix for MALDI-MS imaging of oligosaccharides and lipids in plant tissues |
| title_short | Prefabricated platinum nanomaterial matrix for MALDI-MS imaging of oligosaccharides and lipids in plant tissues |
| title_sort | prefabricated platinum nanomaterial matrix for maldi ms imaging of oligosaccharides and lipids in plant tissues |
| topic | matrix-assisted laser desorption ionization mass spectrometry imaging matrix platinum nanomaterials plant tissue oligosaccharide lipid |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1105374/full |
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