BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin
BECN1/Beclin1 is one of the key proteins in autophagy regulation. However, the biological functions of BECN1 in non-small cell lung cancer (NSCLC) were obscure. Here, we found that neither BECN1 knockdown nor overexpression affected the proliferation of NSCLC cells. Surprisingly, BECN1 overexpressio...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-01-01
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| Series: | Cell Adhesion & Migration |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19336918.2019.1638690 |
| _version_ | 1819091649751416832 |
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| author | Zhujun Cheng Hongbo Xin Tianyu Han |
| author_facet | Zhujun Cheng Hongbo Xin Tianyu Han |
| author_sort | Zhujun Cheng |
| collection | DOAJ |
| description | BECN1/Beclin1 is one of the key proteins in autophagy regulation. However, the biological functions of BECN1 in non-small cell lung cancer (NSCLC) were obscure. Here, we found that neither BECN1 knockdown nor overexpression affected the proliferation of NSCLC cells. Surprisingly, BECN1 overexpression increased cell migration and knocking down BECN1 significantly reduced the migratory ability of NSCLC cells. We further demonstrated that BECN1 could interact with Vimentin and affected its K48-linked ubiquitination. What’s more, BECN1 could also interact with ubiquitin-specific peptidase 14 (USP14), the key de-ubiquitinase of Vimentin, and regulated USP14 mediated de-ubiquitination of Vimentin. Thus, our studies revealed an oncosupportive role of BECN1 in the migration of NSCLC cells through regulating the ubiquitination of Vimentin. |
| first_indexed | 2024-12-21T22:43:05Z |
| format | Article |
| id | doaj.art-43a9e8bff2854f5db4eec5ba0a33be91 |
| institution | Directory Open Access Journal |
| issn | 1933-6918 1933-6926 |
| language | English |
| last_indexed | 2024-12-21T22:43:05Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cell Adhesion & Migration |
| spelling | doaj.art-43a9e8bff2854f5db4eec5ba0a33be912022-12-21T18:47:47ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262019-01-0113124825810.1080/19336918.2019.16386901638690BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of VimentinZhujun Cheng0Hongbo Xin1Tianyu Han2The First Affiliated Hospital of Nanchang UniversityNanchang UniversityThe First Affiliated Hospital of Nanchang UniversityBECN1/Beclin1 is one of the key proteins in autophagy regulation. However, the biological functions of BECN1 in non-small cell lung cancer (NSCLC) were obscure. Here, we found that neither BECN1 knockdown nor overexpression affected the proliferation of NSCLC cells. Surprisingly, BECN1 overexpression increased cell migration and knocking down BECN1 significantly reduced the migratory ability of NSCLC cells. We further demonstrated that BECN1 could interact with Vimentin and affected its K48-linked ubiquitination. What’s more, BECN1 could also interact with ubiquitin-specific peptidase 14 (USP14), the key de-ubiquitinase of Vimentin, and regulated USP14 mediated de-ubiquitination of Vimentin. Thus, our studies revealed an oncosupportive role of BECN1 in the migration of NSCLC cells through regulating the ubiquitination of Vimentin.http://dx.doi.org/10.1080/19336918.2019.1638690becn1migrationnsclcubiquitinationvimentin |
| spellingShingle | Zhujun Cheng Hongbo Xin Tianyu Han BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin Cell Adhesion & Migration becn1 migration nsclc ubiquitination vimentin |
| title | BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin |
| title_full | BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin |
| title_fullStr | BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin |
| title_full_unstemmed | BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin |
| title_short | BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin |
| title_sort | becn1 promotes the migration of nsclc cells through regulating the ubiquitination of vimentin |
| topic | becn1 migration nsclc ubiquitination vimentin |
| url | http://dx.doi.org/10.1080/19336918.2019.1638690 |
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