Alu-deletion in Bangladeshi Women: Scopes of Clinical Utility for the Detection of Early Breast Cancer as a Biomarker
Objective: Alteration of Alu elements of human DNA may result cancer. This study was aimed to find status of Alu-deletion among breast cancer patients. Methods: To identify Alu-deletion, tissue samples from the operated specimen and blood of the breast cancer patients were collected. For comparison...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
West Asia Organization for Cancer Prevention
2022-05-01
|
| Series: | Asian Pacific Journal of Cancer Biology |
| Subjects: | |
| Online Access: | http://www.waocp.com/journal/index.php/apjcb/article/view/750 |
| _version_ | 1797338739715866624 |
|---|---|
| author | Gazi Nurun Nahar Sultana Dilruba Sharmin Iffat Jahan Mohammad Asaduzzaman Rokeya Begum Anindita Paul M Mizanur Rahman |
| author_facet | Gazi Nurun Nahar Sultana Dilruba Sharmin Iffat Jahan Mohammad Asaduzzaman Rokeya Begum Anindita Paul M Mizanur Rahman |
| author_sort | Gazi Nurun Nahar Sultana |
| collection | DOAJ |
| description | Objective: Alteration of Alu elements of human DNA may result cancer. This study was aimed to find status of Alu-deletion among breast cancer patients.
Methods: To identify Alu-deletion, tissue samples from the operated specimen and blood of the breast cancer patients were collected. For comparison, blood was collected from healthy controls. After extraction, DNA was amplified by PCR with Alu specific primer and gel electrophoresis was done. Alu specific banding patterns of DNA of the samples were examined and compared.
Result: Among the 64 patients and equal number (64) of healthy women examined, multiple Alu polymorphic loci have been identified. One of them characterized as deletion at the 2000bp region, found in 83% cancerous blood samples and only 3% in the healthy controls (p < 0.0001), OR 149. Another band was identified as less intensified at the 1700bp, present in 74% cancerous blood samples (p < 0.0001). We also found differential DNA banding pattern between breast cancer (7-9) and control blood samples (9-15) which is highly significant (p < 0.0001). Whereas no such banding pattern was observed in cancer tissues and normal breast tissues collected during surgery.
Conclusion: This study confirms significant presence of Alu-deletion in women with breast cancer in comparison without cancer. Alu-deletion can be presumed as a useful blood-based biomarker for the detection of early breast cancer. |
| first_indexed | 2024-03-08T09:35:39Z |
| format | Article |
| id | doaj.art-43ab1571827f40b5962c28fa58925b88 |
| institution | Directory Open Access Journal |
| issn | 2538-4635 |
| language | English |
| last_indexed | 2024-03-08T09:35:39Z |
| publishDate | 2022-05-01 |
| publisher | West Asia Organization for Cancer Prevention |
| record_format | Article |
| series | Asian Pacific Journal of Cancer Biology |
| spelling | doaj.art-43ab1571827f40b5962c28fa58925b882024-01-30T09:17:19ZengWest Asia Organization for Cancer PreventionAsian Pacific Journal of Cancer Biology2538-46352022-05-0171434910.31557/apjcb.2022.7.1.43-49750Alu-deletion in Bangladeshi Women: Scopes of Clinical Utility for the Detection of Early Breast Cancer as a BiomarkerGazi Nurun Nahar Sultana0Dilruba Sharmin1Iffat Jahan2Mohammad Asaduzzaman3Rokeya Begum4Anindita Paul5M Mizanur Rahman6Centre for Advanced Research in Sciences (CARS) University of Dhaka, BangladeshCentre for Advanced Research in Sciences (CARS) University of Dhaka, BangladeshCentre for Advanced Research in Sciences (CARS) University of Dhaka, BangladeshCentre for Advanced Research in Sciences (CARS) University of Dhaka, BangladeshCentre for Advanced Research in Sciences (CARS) University of Dhaka, BangladeshNational Institute of Cancer Research and Hospital, Dhaka, Bangladesh.National Institute of Cancer Research and Hospital, Dhaka, Bangladesh.Objective: Alteration of Alu elements of human DNA may result cancer. This study was aimed to find status of Alu-deletion among breast cancer patients. Methods: To identify Alu-deletion, tissue samples from the operated specimen and blood of the breast cancer patients were collected. For comparison, blood was collected from healthy controls. After extraction, DNA was amplified by PCR with Alu specific primer and gel electrophoresis was done. Alu specific banding patterns of DNA of the samples were examined and compared. Result: Among the 64 patients and equal number (64) of healthy women examined, multiple Alu polymorphic loci have been identified. One of them characterized as deletion at the 2000bp region, found in 83% cancerous blood samples and only 3% in the healthy controls (p < 0.0001), OR 149. Another band was identified as less intensified at the 1700bp, present in 74% cancerous blood samples (p < 0.0001). We also found differential DNA banding pattern between breast cancer (7-9) and control blood samples (9-15) which is highly significant (p < 0.0001). Whereas no such banding pattern was observed in cancer tissues and normal breast tissues collected during surgery. Conclusion: This study confirms significant presence of Alu-deletion in women with breast cancer in comparison without cancer. Alu-deletion can be presumed as a useful blood-based biomarker for the detection of early breast cancer.http://www.waocp.com/journal/index.php/apjcb/article/view/750gene deletion, alu, dna, breast cancer, biomarker |
| spellingShingle | Gazi Nurun Nahar Sultana Dilruba Sharmin Iffat Jahan Mohammad Asaduzzaman Rokeya Begum Anindita Paul M Mizanur Rahman Alu-deletion in Bangladeshi Women: Scopes of Clinical Utility for the Detection of Early Breast Cancer as a Biomarker Asian Pacific Journal of Cancer Biology gene deletion, alu, dna, breast cancer, biomarker |
| title | Alu-deletion in Bangladeshi Women: Scopes of Clinical Utility for the Detection of Early Breast Cancer as a Biomarker |
| title_full | Alu-deletion in Bangladeshi Women: Scopes of Clinical Utility for the Detection of Early Breast Cancer as a Biomarker |
| title_fullStr | Alu-deletion in Bangladeshi Women: Scopes of Clinical Utility for the Detection of Early Breast Cancer as a Biomarker |
| title_full_unstemmed | Alu-deletion in Bangladeshi Women: Scopes of Clinical Utility for the Detection of Early Breast Cancer as a Biomarker |
| title_short | Alu-deletion in Bangladeshi Women: Scopes of Clinical Utility for the Detection of Early Breast Cancer as a Biomarker |
| title_sort | alu deletion in bangladeshi women scopes of clinical utility for the detection of early breast cancer as a biomarker |
| topic | gene deletion, alu, dna, breast cancer, biomarker |
| url | http://www.waocp.com/journal/index.php/apjcb/article/view/750 |
| work_keys_str_mv | AT gazinurunnaharsultana aludeletioninbangladeshiwomenscopesofclinicalutilityforthedetectionofearlybreastcancerasabiomarker AT dilrubasharmin aludeletioninbangladeshiwomenscopesofclinicalutilityforthedetectionofearlybreastcancerasabiomarker AT iffatjahan aludeletioninbangladeshiwomenscopesofclinicalutilityforthedetectionofearlybreastcancerasabiomarker AT mohammadasaduzzaman aludeletioninbangladeshiwomenscopesofclinicalutilityforthedetectionofearlybreastcancerasabiomarker AT rokeyabegum aludeletioninbangladeshiwomenscopesofclinicalutilityforthedetectionofearlybreastcancerasabiomarker AT aninditapaul aludeletioninbangladeshiwomenscopesofclinicalutilityforthedetectionofearlybreastcancerasabiomarker AT mmizanurrahman aludeletioninbangladeshiwomenscopesofclinicalutilityforthedetectionofearlybreastcancerasabiomarker |