Embryo Rescue Breeding of New Cold-Resistant, Seedless Grapes
Seedlessness in grapes is much appreciated by consumers and especially in cultivars consumed either as table grapes or as raisins. In many parts of the world, low temperature is the main environmental stress limiting grape production. In this study, stenospermocarpic (seed abortion) cultivars were s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/9/992 |
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| author | Yannan Chu Min Li Ruonan Li Kangzhuang Zhang Pengpeng Qiu Xiaojian Yuan Yulei Han Xinyu Liu Yan Xu Guotian Liu |
| author_facet | Yannan Chu Min Li Ruonan Li Kangzhuang Zhang Pengpeng Qiu Xiaojian Yuan Yulei Han Xinyu Liu Yan Xu Guotian Liu |
| author_sort | Yannan Chu |
| collection | DOAJ |
| description | Seedlessness in grapes is much appreciated by consumers and especially in cultivars consumed either as table grapes or as raisins. In many parts of the world, low temperature is the main environmental stress limiting grape production. In this study, stenospermocarpic (seed abortion) cultivars were selected as the female parents while seeded cold-resistant cultivars were selected as the male parents to develop new cold-resistant seedless grapes using embryo rescue technology, which has previously been shown to be a highly efficient way of breeding seedless grapes. Here, we report optima in genotype, sampling time, and culture medium for the embryo rescue of 14 hybrid combinations. Our results indicate that the embryo development rate (39.9%) and the seedlings rate (21.5%) were highest among the 14 crosses when ‘Ruby Seedless’ was used as the female parent and ‘Beibinghong’ was used as the male parent. The best sampling times for ‘Yuehong Wuhe’, ‘Ruby Seedless’, and ‘Melissa seedless’ were 37, 55, and 52 days after flowering, respectively. Embryo rescue efficiency was highest when the sucrose concentration for seedlings was maintained at about 1.0%. Using molecular markers, we detected 91 hybrids with seedless traits and 18 hybrids with cold resistance traits. |
| first_indexed | 2024-03-10T22:42:07Z |
| format | Article |
| id | doaj.art-43ab5e0782924f0ab56df54cd8522e28 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T22:42:07Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-43ab5e0782924f0ab56df54cd8522e282023-11-19T10:58:42ZengMDPI AGHorticulturae2311-75242023-09-019999210.3390/horticulturae9090992Embryo Rescue Breeding of New Cold-Resistant, Seedless GrapesYannan Chu0Min Li1Ruonan Li2Kangzhuang Zhang3Pengpeng Qiu4Xiaojian Yuan5Yulei Han6Xinyu Liu7Yan Xu8Guotian Liu9State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaSeedlessness in grapes is much appreciated by consumers and especially in cultivars consumed either as table grapes or as raisins. In many parts of the world, low temperature is the main environmental stress limiting grape production. In this study, stenospermocarpic (seed abortion) cultivars were selected as the female parents while seeded cold-resistant cultivars were selected as the male parents to develop new cold-resistant seedless grapes using embryo rescue technology, which has previously been shown to be a highly efficient way of breeding seedless grapes. Here, we report optima in genotype, sampling time, and culture medium for the embryo rescue of 14 hybrid combinations. Our results indicate that the embryo development rate (39.9%) and the seedlings rate (21.5%) were highest among the 14 crosses when ‘Ruby Seedless’ was used as the female parent and ‘Beibinghong’ was used as the male parent. The best sampling times for ‘Yuehong Wuhe’, ‘Ruby Seedless’, and ‘Melissa seedless’ were 37, 55, and 52 days after flowering, respectively. Embryo rescue efficiency was highest when the sucrose concentration for seedlings was maintained at about 1.0%. Using molecular markers, we detected 91 hybrids with seedless traits and 18 hybrids with cold resistance traits.https://www.mdpi.com/2311-7524/9/9/992grapeembryo rescue breedingseedless traitscold resistancemolecular marker |
| spellingShingle | Yannan Chu Min Li Ruonan Li Kangzhuang Zhang Pengpeng Qiu Xiaojian Yuan Yulei Han Xinyu Liu Yan Xu Guotian Liu Embryo Rescue Breeding of New Cold-Resistant, Seedless Grapes Horticulturae grape embryo rescue breeding seedless traits cold resistance molecular marker |
| title | Embryo Rescue Breeding of New Cold-Resistant, Seedless Grapes |
| title_full | Embryo Rescue Breeding of New Cold-Resistant, Seedless Grapes |
| title_fullStr | Embryo Rescue Breeding of New Cold-Resistant, Seedless Grapes |
| title_full_unstemmed | Embryo Rescue Breeding of New Cold-Resistant, Seedless Grapes |
| title_short | Embryo Rescue Breeding of New Cold-Resistant, Seedless Grapes |
| title_sort | embryo rescue breeding of new cold resistant seedless grapes |
| topic | grape embryo rescue breeding seedless traits cold resistance molecular marker |
| url | https://www.mdpi.com/2311-7524/9/9/992 |
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