Porphyra haitanensis polysaccharide (PH) attenuates cell hyperplasia via remodeling the cross-talk between Hippo/YAP and mTOR pathways
Porphyra polysaccharide is a special kind of nutrient showing multiple physiological functions including regulating cell proliferation, but the detailed mechanisms are not fully revealed, impairing its further development and applications. This work was to purify and characterize the Porphyra haitan...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-03-01
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| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001562 |
| _version_ | 1797709800527626240 |
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| author | Chong Wang Wanglei Lin Zhihua Sun Yiming Sun Yanbo Wang Linglin Fu |
| author_facet | Chong Wang Wanglei Lin Zhihua Sun Yiming Sun Yanbo Wang Linglin Fu |
| author_sort | Chong Wang |
| collection | DOAJ |
| description | Porphyra polysaccharide is a special kind of nutrient showing multiple physiological functions including regulating cell proliferation, but the detailed mechanisms are not fully revealed, impairing its further development and applications. This work was to purify and characterize the Porphyra haitanensis polysaccharide (PH), investigate its physiological function, and demonstrate the underlying mechanisms. The purified PH was first characterized by Fourier-transform infrared spectroscopy. Then an intestinal epithelial cell model was established, in which PH significantly suppressed cell hyperplasia. Specifically, PH activated the Hippo/YAP pathway, which subsequently activated mTOR pathway, however mTOR activated Hippo in the absence of PH. Moreover, both the inhibition of Hippo by YAP1 knock-down and the suppression of mTOR by rapamycin impaired PH function. These results indicated that PH attenuated hyperplasia activity by remodeling the cross-talk between Hippo/YAP and mTOR pathways, which revealed potential targets and approaches for treating hyperplasia-related diseases and provided novel ways to utilize P. haitanensis as well as other related functional foods. |
| first_indexed | 2024-03-12T06:43:06Z |
| format | Article |
| id | doaj.art-43ac9adef59540da92756865ce1a8b32 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T06:43:06Z |
| publishDate | 2023-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-43ac9adef59540da92756865ce1a8b322023-09-03T00:50:37ZengTsinghua University PressFood Science and Human Wellness2213-45302023-03-01122424430Porphyra haitanensis polysaccharide (PH) attenuates cell hyperplasia via remodeling the cross-talk between Hippo/YAP and mTOR pathwaysChong Wang0Wanglei Lin1Zhihua Sun2Yiming Sun3Yanbo Wang4Linglin Fu5Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaThe Affiliated High School to Hangzhou Normal University, Hangzhou 310030, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China; Corresponding author at: School of Food Science and Biotechnology, Zhejiang Gongshang University Hangzhou 310018, China.Porphyra polysaccharide is a special kind of nutrient showing multiple physiological functions including regulating cell proliferation, but the detailed mechanisms are not fully revealed, impairing its further development and applications. This work was to purify and characterize the Porphyra haitanensis polysaccharide (PH), investigate its physiological function, and demonstrate the underlying mechanisms. The purified PH was first characterized by Fourier-transform infrared spectroscopy. Then an intestinal epithelial cell model was established, in which PH significantly suppressed cell hyperplasia. Specifically, PH activated the Hippo/YAP pathway, which subsequently activated mTOR pathway, however mTOR activated Hippo in the absence of PH. Moreover, both the inhibition of Hippo by YAP1 knock-down and the suppression of mTOR by rapamycin impaired PH function. These results indicated that PH attenuated hyperplasia activity by remodeling the cross-talk between Hippo/YAP and mTOR pathways, which revealed potential targets and approaches for treating hyperplasia-related diseases and provided novel ways to utilize P. haitanensis as well as other related functional foods.http://www.sciencedirect.com/science/article/pii/S2213453022001562Porphyra haitanensisPolysaccharideHippomTORCancer |
| spellingShingle | Chong Wang Wanglei Lin Zhihua Sun Yiming Sun Yanbo Wang Linglin Fu Porphyra haitanensis polysaccharide (PH) attenuates cell hyperplasia via remodeling the cross-talk between Hippo/YAP and mTOR pathways Food Science and Human Wellness Porphyra haitanensis Polysaccharide Hippo mTOR Cancer |
| title | Porphyra haitanensis polysaccharide (PH) attenuates cell hyperplasia via remodeling the cross-talk between Hippo/YAP and mTOR pathways |
| title_full | Porphyra haitanensis polysaccharide (PH) attenuates cell hyperplasia via remodeling the cross-talk between Hippo/YAP and mTOR pathways |
| title_fullStr | Porphyra haitanensis polysaccharide (PH) attenuates cell hyperplasia via remodeling the cross-talk between Hippo/YAP and mTOR pathways |
| title_full_unstemmed | Porphyra haitanensis polysaccharide (PH) attenuates cell hyperplasia via remodeling the cross-talk between Hippo/YAP and mTOR pathways |
| title_short | Porphyra haitanensis polysaccharide (PH) attenuates cell hyperplasia via remodeling the cross-talk between Hippo/YAP and mTOR pathways |
| title_sort | porphyra haitanensis polysaccharide ph attenuates cell hyperplasia via remodeling the cross talk between hippo yap and mtor pathways |
| topic | Porphyra haitanensis Polysaccharide Hippo mTOR Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001562 |
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