Effects of Three Microalgal Diets Varying in LC-PUFA Composition on Growth, <i>Fad</i>, and <i>Elovl</i> Expressions, and Fatty Acid Profiles in Juvenile Razor Clam <i>Sinonovacula constricta</i>
The razor clam <i>Sinonovacula constricta</i> is the first marine mollusk demonstrated to possess the complete long-chain polyunsaturated fatty acids (LC-PUFA) biosynthetic pathway. This study explored the impact of different microalgae on growth, <i>Fad</i> and <i>Elov...
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MDPI AG
2023-09-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/8/10/484 |
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| author | Fei Kong Zhaoshou Ran Haixuan Xie Xuxu Tian Kai Liao Jilin Xu |
| author_facet | Fei Kong Zhaoshou Ran Haixuan Xie Xuxu Tian Kai Liao Jilin Xu |
| author_sort | Fei Kong |
| collection | DOAJ |
| description | The razor clam <i>Sinonovacula constricta</i> is the first marine mollusk demonstrated to possess the complete long-chain polyunsaturated fatty acids (LC-PUFA) biosynthetic pathway. This study explored the impact of different microalgae on growth, <i>Fad</i> and <i>Elovl</i> expressions, and fatty acid (FA) profiles in juvenile <i>S. constricta</i>. Results revealed that juveniles fed with <i>Isochrysis galbana</i> (rich in DHA) or <i>Chaetoceros calcitrans</i> (rich in EPA) consistently exhibited higher growth than those fed <i>Chlorella</i> sp. (rich in LA and ALA), underscoring the importance of dietary LC-PUFA in <i>S. constricta</i>’s development. Expression of most <i>Fad</i> and <i>Elovl</i> in <i>C. calcitrans</i> and <i>I. galbana</i>-fed juveniles were initially up-regulated, then down-regulated, suggesting LC-PUFA demand for faster growth. Although <i>Chlorella</i> sp.-fed juveniles exhibited decreased mRNA levels for most genes, levels were notably higher lately compared to those fed <i>C. calcitrans</i> or <i>I. galbana</i>, hinting at potential LC-PUFA biosynthesis induction. FA profiles in <i>S. constricta</i> generally mirrored those in ingested microalgae, implying direct FA accumulation from diets. Some microalgal FA were absent in farmed <i>S. constricta</i>, while others emerged, indicating <i>S. constricta</i>’s ability to selectively accumulate and synthesize FA. This study enhances the understanding of dietary FA metabolism in <i>S. constricta</i>, valuable for selecting appropriate microalgae in its farming practices. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T21:15:32Z |
| publishDate | 2023-09-01 |
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| series | Fishes |
| spelling | doaj.art-43adbdc442474c36b5088018b3c2e0962023-11-19T16:27:37ZengMDPI AGFishes2410-38882023-09-0181048410.3390/fishes8100484Effects of Three Microalgal Diets Varying in LC-PUFA Composition on Growth, <i>Fad</i>, and <i>Elovl</i> Expressions, and Fatty Acid Profiles in Juvenile Razor Clam <i>Sinonovacula constricta</i>Fei Kong0Zhaoshou Ran1Haixuan Xie2Xuxu Tian3Kai Liao4Jilin Xu5Key Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, ChinaKey Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, ChinaThe razor clam <i>Sinonovacula constricta</i> is the first marine mollusk demonstrated to possess the complete long-chain polyunsaturated fatty acids (LC-PUFA) biosynthetic pathway. This study explored the impact of different microalgae on growth, <i>Fad</i> and <i>Elovl</i> expressions, and fatty acid (FA) profiles in juvenile <i>S. constricta</i>. Results revealed that juveniles fed with <i>Isochrysis galbana</i> (rich in DHA) or <i>Chaetoceros calcitrans</i> (rich in EPA) consistently exhibited higher growth than those fed <i>Chlorella</i> sp. (rich in LA and ALA), underscoring the importance of dietary LC-PUFA in <i>S. constricta</i>’s development. Expression of most <i>Fad</i> and <i>Elovl</i> in <i>C. calcitrans</i> and <i>I. galbana</i>-fed juveniles were initially up-regulated, then down-regulated, suggesting LC-PUFA demand for faster growth. Although <i>Chlorella</i> sp.-fed juveniles exhibited decreased mRNA levels for most genes, levels were notably higher lately compared to those fed <i>C. calcitrans</i> or <i>I. galbana</i>, hinting at potential LC-PUFA biosynthesis induction. FA profiles in <i>S. constricta</i> generally mirrored those in ingested microalgae, implying direct FA accumulation from diets. Some microalgal FA were absent in farmed <i>S. constricta</i>, while others emerged, indicating <i>S. constricta</i>’s ability to selectively accumulate and synthesize FA. This study enhances the understanding of dietary FA metabolism in <i>S. constricta</i>, valuable for selecting appropriate microalgae in its farming practices.https://www.mdpi.com/2410-3888/8/10/484long-chain polyunsaturated fatty acids<i>Fad</i> and <i>Elovl</i>microalgaemarine mollusk |
| spellingShingle | Fei Kong Zhaoshou Ran Haixuan Xie Xuxu Tian Kai Liao Jilin Xu Effects of Three Microalgal Diets Varying in LC-PUFA Composition on Growth, <i>Fad</i>, and <i>Elovl</i> Expressions, and Fatty Acid Profiles in Juvenile Razor Clam <i>Sinonovacula constricta</i> Fishes long-chain polyunsaturated fatty acids <i>Fad</i> and <i>Elovl</i> microalgae marine mollusk |
| title | Effects of Three Microalgal Diets Varying in LC-PUFA Composition on Growth, <i>Fad</i>, and <i>Elovl</i> Expressions, and Fatty Acid Profiles in Juvenile Razor Clam <i>Sinonovacula constricta</i> |
| title_full | Effects of Three Microalgal Diets Varying in LC-PUFA Composition on Growth, <i>Fad</i>, and <i>Elovl</i> Expressions, and Fatty Acid Profiles in Juvenile Razor Clam <i>Sinonovacula constricta</i> |
| title_fullStr | Effects of Three Microalgal Diets Varying in LC-PUFA Composition on Growth, <i>Fad</i>, and <i>Elovl</i> Expressions, and Fatty Acid Profiles in Juvenile Razor Clam <i>Sinonovacula constricta</i> |
| title_full_unstemmed | Effects of Three Microalgal Diets Varying in LC-PUFA Composition on Growth, <i>Fad</i>, and <i>Elovl</i> Expressions, and Fatty Acid Profiles in Juvenile Razor Clam <i>Sinonovacula constricta</i> |
| title_short | Effects of Three Microalgal Diets Varying in LC-PUFA Composition on Growth, <i>Fad</i>, and <i>Elovl</i> Expressions, and Fatty Acid Profiles in Juvenile Razor Clam <i>Sinonovacula constricta</i> |
| title_sort | effects of three microalgal diets varying in lc pufa composition on growth i fad i and i elovl i expressions and fatty acid profiles in juvenile razor clam i sinonovacula constricta i |
| topic | long-chain polyunsaturated fatty acids <i>Fad</i> and <i>Elovl</i> microalgae marine mollusk |
| url | https://www.mdpi.com/2410-3888/8/10/484 |
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