Modest vasomotor dysfunction induced by low doses of C<sub>60 </sub>fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis
<p>Abstract</p> <p>Background</p> <p>Exposure to small size particulate matter in urban air is regarded as a risk factor for cardiovascular effects, whereas there is little information about the impact on the cardiovascular system by exposure to pure carbonaceous materi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-02-01
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| Series: | Particle and Fibre Toxicology |
| Online Access: | http://www.particleandfibretoxicology.com/content/6/1/5 |
| _version_ | 1818766421888335872 |
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| author | Loft Steffen Wallin Håkan Sheykhzade Majid Jacobsen Nicklas R Folkmann Janne K Vesterdal Lise K Møller Peter |
| author_facet | Loft Steffen Wallin Håkan Sheykhzade Majid Jacobsen Nicklas R Folkmann Janne K Vesterdal Lise K Møller Peter |
| author_sort | Loft Steffen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Exposure to small size particulate matter in urban air is regarded as a risk factor for cardiovascular effects, whereas there is little information about the impact on the cardiovascular system by exposure to pure carbonaceous materials in the nano-size range. C<sub>60 </sub>fullerenes are nano-sized particles that are expected to have a widespread use, including cosmetics and medicines.</p> <p>Methods</p> <p>We investigated the association between intraperitoneal injection of pristine C<sub>60 </sub>fullerenes and vasomotor dysfunction in the aorta of 11–13 and 40–42 weeks old apolipoprotein E knockout mice (apoE<sup>-/-</sup>) with different degree of atherosclerosis.</p> <p>Results</p> <p>The aged apoE<sup>-/-</sup>mice had lower endothelium-dependent vasorelaxation elicited by acetylcholine in aorta segments mounted in myographs and the phenylephrine-dependent vasoconstriction response was increased. One hour after an intraperitoneal injection of 0.05 or 0.5 mg/kg of C<sub>60 </sub>fullerenes, the young apoE<sup>-/- </sup>mice had slightly reduced maximal endothelium-dependent vasorelaxation. A similar tendency was observed in the old apoE<sup>-/- </sup>mice. Hampered endothelium-independent vasorelaxation was also observed as slightly increased EC<sub>50 </sub>of sodium nitroprusside-induced vasorelaxation response in young apoE<sup>-/- </sup>mice.</p> <p>Conclusion</p> <p>Treatment with C<sub>60 </sub>fullerenes affected mainly the response to vasorelaxation in young apoE<sup>-/- </sup>mice, whereas the vasomotor dysfunction in old apoE<sup>-/- </sup>mice with more advanced atherosclerosis was less affected by acute C<sub>60 </sub>fullerene treatment. These findings represent an important step in the hazard characterization of C<sub>60 </sub>fullerenes by showing that intraperitoneal administration is associated with a moderate decrease in the vascular function of mice with atherosclerosis.</p> |
| first_indexed | 2024-12-18T08:33:44Z |
| format | Article |
| id | doaj.art-43b0dab0c0814241bbe75d167489d056 |
| institution | Directory Open Access Journal |
| issn | 1743-8977 |
| language | English |
| last_indexed | 2024-12-18T08:33:44Z |
| publishDate | 2009-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Particle and Fibre Toxicology |
| spelling | doaj.art-43b0dab0c0814241bbe75d167489d0562022-12-21T21:14:22ZengBMCParticle and Fibre Toxicology1743-89772009-02-0161510.1186/1743-8977-6-5Modest vasomotor dysfunction induced by low doses of C<sub>60 </sub>fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosisLoft SteffenWallin HåkanSheykhzade MajidJacobsen Nicklas RFolkmann Janne KVesterdal Lise KMøller Peter<p>Abstract</p> <p>Background</p> <p>Exposure to small size particulate matter in urban air is regarded as a risk factor for cardiovascular effects, whereas there is little information about the impact on the cardiovascular system by exposure to pure carbonaceous materials in the nano-size range. C<sub>60 </sub>fullerenes are nano-sized particles that are expected to have a widespread use, including cosmetics and medicines.</p> <p>Methods</p> <p>We investigated the association between intraperitoneal injection of pristine C<sub>60 </sub>fullerenes and vasomotor dysfunction in the aorta of 11–13 and 40–42 weeks old apolipoprotein E knockout mice (apoE<sup>-/-</sup>) with different degree of atherosclerosis.</p> <p>Results</p> <p>The aged apoE<sup>-/-</sup>mice had lower endothelium-dependent vasorelaxation elicited by acetylcholine in aorta segments mounted in myographs and the phenylephrine-dependent vasoconstriction response was increased. One hour after an intraperitoneal injection of 0.05 or 0.5 mg/kg of C<sub>60 </sub>fullerenes, the young apoE<sup>-/- </sup>mice had slightly reduced maximal endothelium-dependent vasorelaxation. A similar tendency was observed in the old apoE<sup>-/- </sup>mice. Hampered endothelium-independent vasorelaxation was also observed as slightly increased EC<sub>50 </sub>of sodium nitroprusside-induced vasorelaxation response in young apoE<sup>-/- </sup>mice.</p> <p>Conclusion</p> <p>Treatment with C<sub>60 </sub>fullerenes affected mainly the response to vasorelaxation in young apoE<sup>-/- </sup>mice, whereas the vasomotor dysfunction in old apoE<sup>-/- </sup>mice with more advanced atherosclerosis was less affected by acute C<sub>60 </sub>fullerene treatment. These findings represent an important step in the hazard characterization of C<sub>60 </sub>fullerenes by showing that intraperitoneal administration is associated with a moderate decrease in the vascular function of mice with atherosclerosis.</p>http://www.particleandfibretoxicology.com/content/6/1/5 |
| spellingShingle | Loft Steffen Wallin Håkan Sheykhzade Majid Jacobsen Nicklas R Folkmann Janne K Vesterdal Lise K Møller Peter Modest vasomotor dysfunction induced by low doses of C<sub>60 </sub>fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis Particle and Fibre Toxicology |
| title | Modest vasomotor dysfunction induced by low doses of C<sub>60 </sub>fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis |
| title_full | Modest vasomotor dysfunction induced by low doses of C<sub>60 </sub>fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis |
| title_fullStr | Modest vasomotor dysfunction induced by low doses of C<sub>60 </sub>fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis |
| title_full_unstemmed | Modest vasomotor dysfunction induced by low doses of C<sub>60 </sub>fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis |
| title_short | Modest vasomotor dysfunction induced by low doses of C<sub>60 </sub>fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis |
| title_sort | modest vasomotor dysfunction induced by low doses of c sub 60 sub fullerenes in apolipoprotein e knockout mice with different degree of atherosclerosis |
| url | http://www.particleandfibretoxicology.com/content/6/1/5 |
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