Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization
Abstract The papillary dermis of human skin is responsible for its biomechanical properties and for supply of epidermis with chemicals. Dermis is mainly composed of structural protein molecules, including collagen and elastin, and contains blood capillaries. Connective tissue diseases, as well as ca...
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| Format: | Article |
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Nature Portfolio
2017-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-01238-w |
| _version_ | 1818343812422959104 |
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| author | Evgeny A. Shirshin Yury I. Gurfinkel Alexander V. Priezzhev Victor V. Fadeev Juergen Lademann Maxim E. Darvin |
| author_facet | Evgeny A. Shirshin Yury I. Gurfinkel Alexander V. Priezzhev Victor V. Fadeev Juergen Lademann Maxim E. Darvin |
| author_sort | Evgeny A. Shirshin |
| collection | DOAJ |
| description | Abstract The papillary dermis of human skin is responsible for its biomechanical properties and for supply of epidermis with chemicals. Dermis is mainly composed of structural protein molecules, including collagen and elastin, and contains blood capillaries. Connective tissue diseases, as well as cardiovascular complications have manifestations on the molecular level in the papillary dermis (e.g. alteration of collagen I and III content) and in the capillary structure. In this paper we assessed the molecular structure of internal and external regions of skin capillaries using two-photon fluorescence lifetime imaging (FLIM) of endogenous compounds. It was shown that the capillaries are characterized by a fast fluorescence decay, which is originated from red blood cells and blood plasma. Using the second harmonic generation signal, FLIM segmentation was performed, which provided for spatial localization and fluorescence decay parameters distribution of collagen I and elastin in the dermal papillae. It was demonstrated that the lifetime distribution was different for the inner area of dermal papillae around the capillary loop that was suggested to be due to collagen III. Hence, we propose a generalized approach to two-photon imaging of the papillary dermis components, which extends the capabilities of this technique in skin diagnosis. |
| first_indexed | 2024-12-13T16:36:32Z |
| format | Article |
| id | doaj.art-43b3bb9674d241428d850a6a938a759e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T16:36:32Z |
| publishDate | 2017-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-43b3bb9674d241428d850a6a938a759e2022-12-21T23:38:23ZengNature PortfolioScientific Reports2045-23222017-04-017111010.1038/s41598-017-01238-wTwo-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localizationEvgeny A. Shirshin0Yury I. Gurfinkel1Alexander V. Priezzhev2Victor V. Fadeev3Juergen Lademann4Maxim E. Darvin5Faculty of Physics, Lomonosov Moscow State UniversityResearch Clinical Center of JSC “Russian Railways”Faculty of Physics, Lomonosov Moscow State UniversityFaculty of Physics, Lomonosov Moscow State UniversityDepartment of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Charité –Universitätsmedizin BerlinDepartment of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Charité –Universitätsmedizin BerlinAbstract The papillary dermis of human skin is responsible for its biomechanical properties and for supply of epidermis with chemicals. Dermis is mainly composed of structural protein molecules, including collagen and elastin, and contains blood capillaries. Connective tissue diseases, as well as cardiovascular complications have manifestations on the molecular level in the papillary dermis (e.g. alteration of collagen I and III content) and in the capillary structure. In this paper we assessed the molecular structure of internal and external regions of skin capillaries using two-photon fluorescence lifetime imaging (FLIM) of endogenous compounds. It was shown that the capillaries are characterized by a fast fluorescence decay, which is originated from red blood cells and blood plasma. Using the second harmonic generation signal, FLIM segmentation was performed, which provided for spatial localization and fluorescence decay parameters distribution of collagen I and elastin in the dermal papillae. It was demonstrated that the lifetime distribution was different for the inner area of dermal papillae around the capillary loop that was suggested to be due to collagen III. Hence, we propose a generalized approach to two-photon imaging of the papillary dermis components, which extends the capabilities of this technique in skin diagnosis.https://doi.org/10.1038/s41598-017-01238-w |
| spellingShingle | Evgeny A. Shirshin Yury I. Gurfinkel Alexander V. Priezzhev Victor V. Fadeev Juergen Lademann Maxim E. Darvin Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization Scientific Reports |
| title | Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization |
| title_full | Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization |
| title_fullStr | Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization |
| title_full_unstemmed | Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization |
| title_short | Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization |
| title_sort | two photon autofluorescence lifetime imaging of human skin papillary dermis in vivo assessment of blood capillaries and structural proteins localization |
| url | https://doi.org/10.1038/s41598-017-01238-w |
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