Investigation of Protective Effect of Hypericum Perforatum on Ethanol-Induced Acute Gastric Mucosal Injury in Rats
Aim:This study was conducted to evaluate the antiapoptototic and antiinflammatory effect of Hypericum perforatum in the rats induced gastric ulcer by absolute ethanol.Material and Methods:Forty male Wistar albino rats were used in this study. Rats were divided four group, randomly. Group I (Control,...
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| Format: | Article |
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Galenos Yayincilik
2017-12-01
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| Series: | Namık Kemal Tıp Dergisi |
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http://namikkemalmedj.com/archives/archive-detail/article-preview/nvestigation-of-protective-effect-of-hypericum-per/40413
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| author | İhsan KARABOĞA Ayşe Handan DÖKMECİ Mehmet Akif OVALI Ahsen YILMAZ |
| author_facet | İhsan KARABOĞA Ayşe Handan DÖKMECİ Mehmet Akif OVALI Ahsen YILMAZ |
| author_sort | İhsan KARABOĞA |
| collection | DOAJ |
| description | Aim:This study was conducted to evaluate the antiapoptototic and antiinflammatory effect of Hypericum perforatum in the rats induced gastric ulcer by absolute ethanol.Material and Methods:Forty male Wistar albino rats were used in this study. Rats were divided four group, randomly. Group I (Control, n=10), Group II (Ethanol, n=10, 1 ml ethanol, orally, 90 min) Group III (H. perforatum + ethanol, 1 ml H. perforatum, 120 min; 1 ml ethanol, 90 min, orally), Group IV (Olive oil + ethanol, 1 ml olive oil, 120 min; 1 ml ethanol, 90 min, orally). Gastric ulcer score was determined in gastric mucosa by morphometrically. Histopatological staining, Immunohistochemical iNOS and PCNA staining, apoptotic TUNEL staining, gastric IL-10 and IL-6 expression (ELISA) and SOD, MDA, CAT levels were assessed in gastric tissue.Results:Elevated ulcer index and increased IL-6, iNOS, TUNEL and MDA levels were detected in the ethanol-induced gastric injury model (grup II). Pre-treatment of H. perforatum and olive oil significantly attenuated the gastric lesions as compared to the group II.Conclusion:It was concluded that H. perforatum and olive oil may represents a potential therapeutic option to reduce the risk of gastric mucosal ulceration. |
| first_indexed | 2024-04-10T11:58:53Z |
| format | Article |
| id | doaj.art-43b62edb6b6f4d7f9ef540ad12c762e9 |
| institution | Directory Open Access Journal |
| issn | 2587-0262 |
| language | English |
| last_indexed | 2024-04-10T11:58:53Z |
| publishDate | 2017-12-01 |
| publisher | Galenos Yayincilik |
| record_format | Article |
| series | Namık Kemal Tıp Dergisi |
| spelling | doaj.art-43b62edb6b6f4d7f9ef540ad12c762e92023-02-15T16:16:41ZengGalenos YayincilikNamık Kemal Tıp Dergisi2587-02622017-12-01539910813049054Investigation of Protective Effect of Hypericum Perforatum on Ethanol-Induced Acute Gastric Mucosal Injury in Ratsİhsan KARABOĞA0Ayşe Handan DÖKMECİ1Mehmet Akif OVALI2Ahsen YILMAZ3 Namık Kemal Üniversitesi, Sağlık Yüksekokulu, Acil Yardım ve Afet Yönetimi Bölümü, Tekirdağ, Türkiye Namık Kemal Üniversitesi, Sağlık Yüksekokulu, Acil Yardım ve Afet Yönetimi Bölümü, Tekirdağ, Türkiye Çanakkale Onsekiz Mart Üniversitesi, Tıp Fakültesi, Fizyoloji ABD, Çanakkale, Türkiye Namık Kemal Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya ABD, Tekirdağ, Türkiye Aim:This study was conducted to evaluate the antiapoptototic and antiinflammatory effect of Hypericum perforatum in the rats induced gastric ulcer by absolute ethanol.Material and Methods:Forty male Wistar albino rats were used in this study. Rats were divided four group, randomly. Group I (Control, n=10), Group II (Ethanol, n=10, 1 ml ethanol, orally, 90 min) Group III (H. perforatum + ethanol, 1 ml H. perforatum, 120 min; 1 ml ethanol, 90 min, orally), Group IV (Olive oil + ethanol, 1 ml olive oil, 120 min; 1 ml ethanol, 90 min, orally). Gastric ulcer score was determined in gastric mucosa by morphometrically. Histopatological staining, Immunohistochemical iNOS and PCNA staining, apoptotic TUNEL staining, gastric IL-10 and IL-6 expression (ELISA) and SOD, MDA, CAT levels were assessed in gastric tissue.Results:Elevated ulcer index and increased IL-6, iNOS, TUNEL and MDA levels were detected in the ethanol-induced gastric injury model (grup II). Pre-treatment of H. perforatum and olive oil significantly attenuated the gastric lesions as compared to the group II.Conclusion:It was concluded that H. perforatum and olive oil may represents a potential therapeutic option to reduce the risk of gastric mucosal ulceration. http://namikkemalmedj.com/archives/archive-detail/article-preview/nvestigation-of-protective-effect-of-hypericum-per/40413 gastric ulcerhypericum perforatuminostunelinterleukin-6interleukin-10 |
| spellingShingle | İhsan KARABOĞA Ayşe Handan DÖKMECİ Mehmet Akif OVALI Ahsen YILMAZ Investigation of Protective Effect of Hypericum Perforatum on Ethanol-Induced Acute Gastric Mucosal Injury in Rats Namık Kemal Tıp Dergisi gastric ulcer hypericum perforatum inos tunel interleukin-6 interleukin-10 |
| title | Investigation of Protective Effect of Hypericum Perforatum on Ethanol-Induced Acute Gastric Mucosal Injury in Rats |
| title_full | Investigation of Protective Effect of Hypericum Perforatum on Ethanol-Induced Acute Gastric Mucosal Injury in Rats |
| title_fullStr | Investigation of Protective Effect of Hypericum Perforatum on Ethanol-Induced Acute Gastric Mucosal Injury in Rats |
| title_full_unstemmed | Investigation of Protective Effect of Hypericum Perforatum on Ethanol-Induced Acute Gastric Mucosal Injury in Rats |
| title_short | Investigation of Protective Effect of Hypericum Perforatum on Ethanol-Induced Acute Gastric Mucosal Injury in Rats |
| title_sort | investigation of protective effect of hypericum perforatum on ethanol induced acute gastric mucosal injury in rats |
| topic | gastric ulcer hypericum perforatum inos tunel interleukin-6 interleukin-10 |
| url |
http://namikkemalmedj.com/archives/archive-detail/article-preview/nvestigation-of-protective-effect-of-hypericum-per/40413
|
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