Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncture
To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability and apoptosis were analyzed using MTT assay, tryphan blue assay, [3H]thymidine release assay, flow cytometric analysis, and activity of caspase-3 protease acti...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Korean Pharmacopuncture Institute
2000-07-01
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| Series: | Journal of Pharmacopuncture |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3831/KPI.2000.3.1.001 |
| _version_ | 1811287874008514560 |
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| author | Park Chan-Yol Seo Jung-Chul Choi Do-Young Ahn Byoung-Choul |
| author_facet | Park Chan-Yol Seo Jung-Chul Choi Do-Young Ahn Byoung-Choul |
| author_sort | Park Chan-Yol |
| collection | DOAJ |
| description | To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability and apoptosis were analyzed using MTT assay, tryphan blue assay, [3H]thymidine release assay, flow cytometric analysis, and activity of caspase-3 protease activity assay. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis-related genes was performed.
The obtained results are summarized as follows:
1. The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner.
2. Significant induction of apoptosis was identified using tryphan blue assay, [3H]thymidine release assay, and flow cytometric analysis of sub G1 fraction.
3. In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner.
4. Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-XL were down-regulated whereas Bax was up-regulated by bee venom treatment. |
| first_indexed | 2024-04-13T03:26:05Z |
| format | Article |
| id | doaj.art-43b809c9656b4a57b07dee8bac316a8c |
| institution | Directory Open Access Journal |
| issn | 2093-6966 2234-6856 |
| language | English |
| last_indexed | 2024-04-13T03:26:05Z |
| publishDate | 2000-07-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-43b809c9656b4a57b07dee8bac316a8c2022-12-22T03:04:38ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562000-07-013111910.3831/KPI.2000.3.1.001Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncturePark Chan-Yol 0Seo Jung-Chul1Choi Do-Young2Ahn Byoung-Choul 3Dept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee UniversityDept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee UniversityDept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee UniversityDept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee UniversityTo study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability and apoptosis were analyzed using MTT assay, tryphan blue assay, [3H]thymidine release assay, flow cytometric analysis, and activity of caspase-3 protease activity assay. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis-related genes was performed. The obtained results are summarized as follows: 1. The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2. Significant induction of apoptosis was identified using tryphan blue assay, [3H]thymidine release assay, and flow cytometric analysis of sub G1 fraction. 3. In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4. Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-XL were down-regulated whereas Bax was up-regulated by bee venom treatment.http://dx.doi.org/10.3831/KPI.2000.3.1.001been venomanti-cancer effectmolecular biology |
| spellingShingle | Park Chan-Yol Seo Jung-Chul Choi Do-Young Ahn Byoung-Choul Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncture Journal of Pharmacopuncture been venom anti-cancer effect molecular biology |
| title | Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncture |
| title_full | Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncture |
| title_fullStr | Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncture |
| title_full_unstemmed | Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncture |
| title_short | Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncture |
| title_sort | molecular biological study of anti cancer effects of bee venom aqua acupuncture |
| topic | been venom anti-cancer effect molecular biology |
| url | http://dx.doi.org/10.3831/KPI.2000.3.1.001 |
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