Recruitment of Cytosolic J-Proteins by TOM Receptors Promotes Mitochondrial Protein Biogenesis
Summary: Mitochondria possess elaborate machineries for the import of proteins from the cytosol. Cytosolic factors like Hsp70 chaperones and their co-chaperones, the J-proteins, guide proteins to the mitochondrial surface. The translocase of the mitochondrial outer membrane (TOM) forms the entry gat...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-11-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124718316875 |
| _version_ | 1818888409390776320 |
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| author | Łukasz Opaliński Jiyao Song Chantal Priesnitz Lena-Sophie Wenz Silke Oeljeklaus Bettina Warscheid Nikolaus Pfanner Thomas Becker |
| author_facet | Łukasz Opaliński Jiyao Song Chantal Priesnitz Lena-Sophie Wenz Silke Oeljeklaus Bettina Warscheid Nikolaus Pfanner Thomas Becker |
| author_sort | Łukasz Opaliński |
| collection | DOAJ |
| description | Summary: Mitochondria possess elaborate machineries for the import of proteins from the cytosol. Cytosolic factors like Hsp70 chaperones and their co-chaperones, the J-proteins, guide proteins to the mitochondrial surface. The translocase of the mitochondrial outer membrane (TOM) forms the entry gate for preproteins. How the proteins are delivered to mitochondrial preprotein receptors is poorly understood. We identify the cytosolic J-protein Xdj1 as a specific interaction partner of the central receptor Tom22. Tom22 recruits Xdj1 to the mitochondrial surface to promote import of preproteins and assembly of the TOM complex. Additionally, we find that the receptor Tom70 binds a different cytosolic J-protein, Djp1. Our findings suggest that cytosolic J-proteins target distinct TOM receptors and promote the biogenesis of mitochondrial proteins. : Opaliński et al. report that mitochondrial protein import receptors selectively recognize J-protein co-chaperones of the cytosol. The co-chaperones bind hydrophobic precursor proteins and assist in transferring them to the receptors of the mitochondrial protein entry gate. Keywords: mitochondria, TOM complex, protein targeting, J-proteins, cytosolic chaperones |
| first_indexed | 2024-12-19T16:52:40Z |
| format | Article |
| id | doaj.art-43bdfad4d5684abd854c0d810388b7bb |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-19T16:52:40Z |
| publishDate | 2018-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-43bdfad4d5684abd854c0d810388b7bb2022-12-21T20:13:30ZengElsevierCell Reports2211-12472018-11-0125820362043.e5Recruitment of Cytosolic J-Proteins by TOM Receptors Promotes Mitochondrial Protein BiogenesisŁukasz Opaliński0Jiyao Song1Chantal Priesnitz2Lena-Sophie Wenz3Silke Oeljeklaus4Bettina Warscheid5Nikolaus Pfanner6Thomas Becker7Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, 79104 Freiburg, GermanyInstitute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, 79104 Freiburg, GermanyInstitute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany; Faculty of Biology, University of Freiburg, 79104 Freiburg, GermanyInstitute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, 79104 Freiburg, GermanyInstitute of Biology II, Biochemistry and Functional Proteomics, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany; BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg, GermanyInstitute of Biology II, Biochemistry and Functional Proteomics, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany; BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg, GermanyInstitute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany; BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg, Germany; Corresponding authorInstitute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany; BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg, Germany; Corresponding authorSummary: Mitochondria possess elaborate machineries for the import of proteins from the cytosol. Cytosolic factors like Hsp70 chaperones and their co-chaperones, the J-proteins, guide proteins to the mitochondrial surface. The translocase of the mitochondrial outer membrane (TOM) forms the entry gate for preproteins. How the proteins are delivered to mitochondrial preprotein receptors is poorly understood. We identify the cytosolic J-protein Xdj1 as a specific interaction partner of the central receptor Tom22. Tom22 recruits Xdj1 to the mitochondrial surface to promote import of preproteins and assembly of the TOM complex. Additionally, we find that the receptor Tom70 binds a different cytosolic J-protein, Djp1. Our findings suggest that cytosolic J-proteins target distinct TOM receptors and promote the biogenesis of mitochondrial proteins. : Opaliński et al. report that mitochondrial protein import receptors selectively recognize J-protein co-chaperones of the cytosol. The co-chaperones bind hydrophobic precursor proteins and assist in transferring them to the receptors of the mitochondrial protein entry gate. Keywords: mitochondria, TOM complex, protein targeting, J-proteins, cytosolic chaperoneshttp://www.sciencedirect.com/science/article/pii/S2211124718316875 |
| spellingShingle | Łukasz Opaliński Jiyao Song Chantal Priesnitz Lena-Sophie Wenz Silke Oeljeklaus Bettina Warscheid Nikolaus Pfanner Thomas Becker Recruitment of Cytosolic J-Proteins by TOM Receptors Promotes Mitochondrial Protein Biogenesis Cell Reports |
| title | Recruitment of Cytosolic J-Proteins by TOM Receptors Promotes Mitochondrial Protein Biogenesis |
| title_full | Recruitment of Cytosolic J-Proteins by TOM Receptors Promotes Mitochondrial Protein Biogenesis |
| title_fullStr | Recruitment of Cytosolic J-Proteins by TOM Receptors Promotes Mitochondrial Protein Biogenesis |
| title_full_unstemmed | Recruitment of Cytosolic J-Proteins by TOM Receptors Promotes Mitochondrial Protein Biogenesis |
| title_short | Recruitment of Cytosolic J-Proteins by TOM Receptors Promotes Mitochondrial Protein Biogenesis |
| title_sort | recruitment of cytosolic j proteins by tom receptors promotes mitochondrial protein biogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2211124718316875 |
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