The Validation of a Non-Invasive Skin Sampling Device for Detecting Cetacean Poxvirus
Poxvirus-like lesions are widely used as a potential health indicator in cetaceans, although for this application, corroboration of Poxvirus skin disease is imperative. Aiming to address skin biopsies intrusiveness, a preliminary investigation of a non-invasive skin sampling procedure to molecularly...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/10/2814 |
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| author | Simone Segura-Göthlin Antonio Fernández Manuel Arbelo Idaira Felipe-Jiménez Ana Colom-Rivero Javier Almunia Eva Sierra |
| author_facet | Simone Segura-Göthlin Antonio Fernández Manuel Arbelo Idaira Felipe-Jiménez Ana Colom-Rivero Javier Almunia Eva Sierra |
| author_sort | Simone Segura-Göthlin |
| collection | DOAJ |
| description | Poxvirus-like lesions are widely used as a potential health indicator in cetaceans, although for this application, corroboration of Poxvirus skin disease is imperative. Aiming to address skin biopsies intrusiveness, a preliminary investigation of a non-invasive skin sampling procedure to molecularly detect CePV-1 in 12 tattoo-like-lesions from two free-ranging stranded cetaceans in the Canary Islands was performed. Skin lesions were brushed with cytology cell samplers (CCSs) and placed into 1.5 mL microcentrifuge tubes with 1 mL of RNA<i>later</i><sup>TM</sup> Stabilization Solution. For factual comparisons, DNA extractions from sloughed skin obtained with CCS and biopsies from the same lesions were accomplished with DNA Tissue Kit S<sup>TM</sup> (QuickGene, Kurabo, Japan). Moreover, a second DNA extraction from sloughed skin with DNeasy<sup>TM</sup> Blood and Tissue Kit (Qiagen, Inc., Valencia, CA, USA) was performed to ascertain kit suitability for CCS. Molecular detection of CePV-1 was performed through a real-time PCR. As a result, a 91.7% and 83.3% rates of positivity were obtained with biopsies and CCS through Quickgene, respectively, compared to the rate of 100% using CCS with Qiagen. Accordingly, CCS is a reliable non-invasive sampling device to obtain sufficient genetic material to be analyzed for CePV-1 in tattoo-skin-lesions as well as for other purposes in cetaceans under human care. |
| first_indexed | 2024-03-10T06:47:08Z |
| format | Article |
| id | doaj.art-43c563ddd77a4580918d7899c546a532 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T06:47:08Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-43c563ddd77a4580918d7899c546a5322023-11-22T17:09:32ZengMDPI AGAnimals2076-26152021-09-011110281410.3390/ani11102814The Validation of a Non-Invasive Skin Sampling Device for Detecting Cetacean PoxvirusSimone Segura-Göthlin0Antonio Fernández1Manuel Arbelo2Idaira Felipe-Jiménez3Ana Colom-Rivero4Javier Almunia5Eva Sierra6Veterinary Histology and Pathology, Atlantic Center for Cetacean Research, University Institute of Animal Health and Food Safety (IUSA), Veterinary School, University of Las Palmas de Gran Canaria (ULPGC), Trasmontaña, s/n, 35413 Las Palmas, SpainVeterinary Histology and Pathology, Atlantic Center for Cetacean Research, University Institute of Animal Health and Food Safety (IUSA), Veterinary School, University of Las Palmas de Gran Canaria (ULPGC), Trasmontaña, s/n, 35413 Las Palmas, SpainVeterinary Histology and Pathology, Atlantic Center for Cetacean Research, University Institute of Animal Health and Food Safety (IUSA), Veterinary School, University of Las Palmas de Gran Canaria (ULPGC), Trasmontaña, s/n, 35413 Las Palmas, SpainVeterinary Histology and Pathology, Atlantic Center for Cetacean Research, University Institute of Animal Health and Food Safety (IUSA), Veterinary School, University of Las Palmas de Gran Canaria (ULPGC), Trasmontaña, s/n, 35413 Las Palmas, SpainVeterinary Histology and Pathology, Atlantic Center for Cetacean Research, University Institute of Animal Health and Food Safety (IUSA), Veterinary School, University of Las Palmas de Gran Canaria (ULPGC), Trasmontaña, s/n, 35413 Las Palmas, SpainLoro Parque Foundation, Avda. Loro Parque, s/n, 38400 Puerto de la Cruz, SpainVeterinary Histology and Pathology, Atlantic Center for Cetacean Research, University Institute of Animal Health and Food Safety (IUSA), Veterinary School, University of Las Palmas de Gran Canaria (ULPGC), Trasmontaña, s/n, 35413 Las Palmas, SpainPoxvirus-like lesions are widely used as a potential health indicator in cetaceans, although for this application, corroboration of Poxvirus skin disease is imperative. Aiming to address skin biopsies intrusiveness, a preliminary investigation of a non-invasive skin sampling procedure to molecularly detect CePV-1 in 12 tattoo-like-lesions from two free-ranging stranded cetaceans in the Canary Islands was performed. Skin lesions were brushed with cytology cell samplers (CCSs) and placed into 1.5 mL microcentrifuge tubes with 1 mL of RNA<i>later</i><sup>TM</sup> Stabilization Solution. For factual comparisons, DNA extractions from sloughed skin obtained with CCS and biopsies from the same lesions were accomplished with DNA Tissue Kit S<sup>TM</sup> (QuickGene, Kurabo, Japan). Moreover, a second DNA extraction from sloughed skin with DNeasy<sup>TM</sup> Blood and Tissue Kit (Qiagen, Inc., Valencia, CA, USA) was performed to ascertain kit suitability for CCS. Molecular detection of CePV-1 was performed through a real-time PCR. As a result, a 91.7% and 83.3% rates of positivity were obtained with biopsies and CCS through Quickgene, respectively, compared to the rate of 100% using CCS with Qiagen. Accordingly, CCS is a reliable non-invasive sampling device to obtain sufficient genetic material to be analyzed for CePV-1 in tattoo-skin-lesions as well as for other purposes in cetaceans under human care.https://www.mdpi.com/2076-2615/11/10/2814cetacean poxvirusskin lesionshealth indicatorwelfarebiopsycytology cell sampler |
| spellingShingle | Simone Segura-Göthlin Antonio Fernández Manuel Arbelo Idaira Felipe-Jiménez Ana Colom-Rivero Javier Almunia Eva Sierra The Validation of a Non-Invasive Skin Sampling Device for Detecting Cetacean Poxvirus Animals cetacean poxvirus skin lesions health indicator welfare biopsy cytology cell sampler |
| title | The Validation of a Non-Invasive Skin Sampling Device for Detecting Cetacean Poxvirus |
| title_full | The Validation of a Non-Invasive Skin Sampling Device for Detecting Cetacean Poxvirus |
| title_fullStr | The Validation of a Non-Invasive Skin Sampling Device for Detecting Cetacean Poxvirus |
| title_full_unstemmed | The Validation of a Non-Invasive Skin Sampling Device for Detecting Cetacean Poxvirus |
| title_short | The Validation of a Non-Invasive Skin Sampling Device for Detecting Cetacean Poxvirus |
| title_sort | validation of a non invasive skin sampling device for detecting cetacean poxvirus |
| topic | cetacean poxvirus skin lesions health indicator welfare biopsy cytology cell sampler |
| url | https://www.mdpi.com/2076-2615/11/10/2814 |
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