Three-Dimensional Co-Culture System of Human Osteoblasts and Osteoclast Precursors from Osteoporotic Patients as an Innovative Model to Study the Role of Nutrients: Focus on Vitamin K2
Several natural compounds, such as vitamin K2, have been highlighted for their positive effects on bone metabolism. It has been proposed that skeletal disorders, such as osteoporosis, may benefit from vitamin K2-based therapies or its regular intake. However, further studies are needed to better cla...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/8/2823 |
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| author | Domitilla Mandatori Letizia Penolazzi Letizia Pelusi Elisabetta Lambertini Francesca Michelucci Annamaria Porreca Pietro Cerritelli Caterina Pipino Angelo Di Iorio Danilo Bruni Marta Di Nicola Roberto Buda Roberta Piva Assunta Pandolfi |
| author_facet | Domitilla Mandatori Letizia Penolazzi Letizia Pelusi Elisabetta Lambertini Francesca Michelucci Annamaria Porreca Pietro Cerritelli Caterina Pipino Angelo Di Iorio Danilo Bruni Marta Di Nicola Roberto Buda Roberta Piva Assunta Pandolfi |
| author_sort | Domitilla Mandatori |
| collection | DOAJ |
| description | Several natural compounds, such as vitamin K2, have been highlighted for their positive effects on bone metabolism. It has been proposed that skeletal disorders, such as osteoporosis, may benefit from vitamin K2-based therapies or its regular intake. However, further studies are needed to better clarify the effects of vitamin K2 in bone disorders. To this aim, we developed in vitro a three-dimensional (3D) cell culture system one step closer to the bone microenvironment based on co-culturing osteoblasts and osteoclasts precursors obtained from bone specimens and peripheral blood of the same osteoporotic patient, respectively. Such a 3-D co-culture system was more informative than the traditional 2-D cell cultures when responsiveness to vitamin K2 was analyzed, paving the way for data interpretation on single patients. Following this approach, the anabolic effects of vitamin K2 on the osteoblast counterpart were found to be correlated with bone turnover markers measured in osteoporotic patients’ sera. Overall, our data suggest that co-cultured osteoblasts and osteoclast precursors from the same osteoporotic patient may be suitable to generate an in vitro 3-D experimental model that potentially reflects the individual’s bone metabolism and may be useful to predict personal responsiveness to nutraceutical or drug molecules designed to positively affect bone health. |
| first_indexed | 2024-03-10T08:30:06Z |
| format | Article |
| id | doaj.art-43c7b31bfb9942168aa971926e22f1a6 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T08:30:06Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-43c7b31bfb9942168aa971926e22f1a62023-11-22T09:06:39ZengMDPI AGNutrients2072-66432021-08-01138282310.3390/nu13082823Three-Dimensional Co-Culture System of Human Osteoblasts and Osteoclast Precursors from Osteoporotic Patients as an Innovative Model to Study the Role of Nutrients: Focus on Vitamin K2Domitilla Mandatori0Letizia Penolazzi1Letizia Pelusi2Elisabetta Lambertini3Francesca Michelucci4Annamaria Porreca5Pietro Cerritelli6Caterina Pipino7Angelo Di Iorio8Danilo Bruni9Marta Di Nicola10Roberto Buda11Roberta Piva12Assunta Pandolfi13Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Neuroscience and Rehabilitation, University of Ferrara, 44121 Ferrara, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Neuroscience and Rehabilitation, University of Ferrara, 44121 Ferrara, ItalyDepartment of Medicine and Aging Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medicine and Aging Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medicine and Aging Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medicine and Aging Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medicine and Aging Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Neuroscience and Rehabilitation, University of Ferrara, 44121 Ferrara, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalySeveral natural compounds, such as vitamin K2, have been highlighted for their positive effects on bone metabolism. It has been proposed that skeletal disorders, such as osteoporosis, may benefit from vitamin K2-based therapies or its regular intake. However, further studies are needed to better clarify the effects of vitamin K2 in bone disorders. To this aim, we developed in vitro a three-dimensional (3D) cell culture system one step closer to the bone microenvironment based on co-culturing osteoblasts and osteoclasts precursors obtained from bone specimens and peripheral blood of the same osteoporotic patient, respectively. Such a 3-D co-culture system was more informative than the traditional 2-D cell cultures when responsiveness to vitamin K2 was analyzed, paving the way for data interpretation on single patients. Following this approach, the anabolic effects of vitamin K2 on the osteoblast counterpart were found to be correlated with bone turnover markers measured in osteoporotic patients’ sera. Overall, our data suggest that co-cultured osteoblasts and osteoclast precursors from the same osteoporotic patient may be suitable to generate an in vitro 3-D experimental model that potentially reflects the individual’s bone metabolism and may be useful to predict personal responsiveness to nutraceutical or drug molecules designed to positively affect bone health.https://www.mdpi.com/2072-6643/13/8/2823osteoporosisbone healthosteoblastsosteoclasts3-D culturevitamin K2 |
| spellingShingle | Domitilla Mandatori Letizia Penolazzi Letizia Pelusi Elisabetta Lambertini Francesca Michelucci Annamaria Porreca Pietro Cerritelli Caterina Pipino Angelo Di Iorio Danilo Bruni Marta Di Nicola Roberto Buda Roberta Piva Assunta Pandolfi Three-Dimensional Co-Culture System of Human Osteoblasts and Osteoclast Precursors from Osteoporotic Patients as an Innovative Model to Study the Role of Nutrients: Focus on Vitamin K2 Nutrients osteoporosis bone health osteoblasts osteoclasts 3-D culture vitamin K2 |
| title | Three-Dimensional Co-Culture System of Human Osteoblasts and Osteoclast Precursors from Osteoporotic Patients as an Innovative Model to Study the Role of Nutrients: Focus on Vitamin K2 |
| title_full | Three-Dimensional Co-Culture System of Human Osteoblasts and Osteoclast Precursors from Osteoporotic Patients as an Innovative Model to Study the Role of Nutrients: Focus on Vitamin K2 |
| title_fullStr | Three-Dimensional Co-Culture System of Human Osteoblasts and Osteoclast Precursors from Osteoporotic Patients as an Innovative Model to Study the Role of Nutrients: Focus on Vitamin K2 |
| title_full_unstemmed | Three-Dimensional Co-Culture System of Human Osteoblasts and Osteoclast Precursors from Osteoporotic Patients as an Innovative Model to Study the Role of Nutrients: Focus on Vitamin K2 |
| title_short | Three-Dimensional Co-Culture System of Human Osteoblasts and Osteoclast Precursors from Osteoporotic Patients as an Innovative Model to Study the Role of Nutrients: Focus on Vitamin K2 |
| title_sort | three dimensional co culture system of human osteoblasts and osteoclast precursors from osteoporotic patients as an innovative model to study the role of nutrients focus on vitamin k2 |
| topic | osteoporosis bone health osteoblasts osteoclasts 3-D culture vitamin K2 |
| url | https://www.mdpi.com/2072-6643/13/8/2823 |
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