Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells
Shuying Hu,1 Hanbang Chen,1 Xuefeng Zhou,2 Gang Chen,1 Ke Hu,3 Yi Cheng,1 Lili Wang,1 Feimin Zhang1 1Jiangsu Key Laboratory of Oral Diseases, Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China; 2State Key Laboratory of Bioelectronics,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2018-07-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/thermally-induced-self-agglomeration-3d-scaffolds-with-bmp-2-loaded-co-peer-reviewed-article-IJN |
| _version_ | 1818421601182416896 |
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| author | Hu S Chen H Zhou X Chen G Hu K Cheng Y Wang L Zhang F |
| author_facet | Hu S Chen H Zhou X Chen G Hu K Cheng Y Wang L Zhang F |
| author_sort | Hu S |
| collection | DOAJ |
| description | Shuying Hu,1 Hanbang Chen,1 Xuefeng Zhou,2 Gang Chen,1 Ke Hu,3 Yi Cheng,1 Lili Wang,1 Feimin Zhang1 1Jiangsu Key Laboratory of Oral Diseases, Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China; 2State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China; 3Key Laboratory of Clinical and Medical Engineering, Department of Biomedical Engineering, School of Basic Medical Science, Nanjing Medical University, Nanjing 210000, China Introduction: Scaffold structure plays a vital role in cell behaviors. Compared with two-dimensional structure, 3D scaffolds can mimic natural extracellular matrix (ECM) and promote cell–cell and cell–matrix interactions. The combination of osteoconductive scaffolds and osteoinductive growth factors is considered to have synergistic effects on bone regeneration. Materials and methods: In this study, core–shell poly(lactide-co-glycolide) (PLGA)/polycaprolactone (PCL)–BMP-2 (PP–B) fibrous scaffolds were prepared through coaxial electrospinning. Next, we fabricated 3D scaffolds based on PP–B fibers with thermally induced self-agglomeration (TISA) method and compared with conventional PLGA/PCL scaffolds in terms of scaffold morphology and BMP-2 release behaviors. Then, rat adipose-derived stem cells (rADSCs) were seeded on the scaffolds, and the effects on cell proliferation, cell morphology, and osteogenic differentiation of rADSCs were detected. Results: The results demonstrated that 3D scaffold incorporated with BMP-2 significantly increased proliferation and osteogenic differentiation of rADSCs, followed by PP–B group. Conclusion: Our findings indicate that scaffolds with 3D structure and osteoinductive growth factors have great potential in bone tissue engineering. Keywords: BMP-2, core–shell scaffolds, 3D fibrous scaffolds, osteogenic differentiation |
| first_indexed | 2024-12-14T13:12:57Z |
| format | Article |
| id | doaj.art-43c88c12fbd647e09773614f38914449 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-14T13:12:57Z |
| publishDate | 2018-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-43c88c12fbd647e09773614f389144492022-12-21T23:00:08ZengDove Medical PressInternational Journal of Nanomedicine1178-20132018-07-01Volume 134145415539368Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cellsHu SChen HZhou XChen GHu KCheng YWang LZhang FShuying Hu,1 Hanbang Chen,1 Xuefeng Zhou,2 Gang Chen,1 Ke Hu,3 Yi Cheng,1 Lili Wang,1 Feimin Zhang1 1Jiangsu Key Laboratory of Oral Diseases, Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China; 2State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China; 3Key Laboratory of Clinical and Medical Engineering, Department of Biomedical Engineering, School of Basic Medical Science, Nanjing Medical University, Nanjing 210000, China Introduction: Scaffold structure plays a vital role in cell behaviors. Compared with two-dimensional structure, 3D scaffolds can mimic natural extracellular matrix (ECM) and promote cell–cell and cell–matrix interactions. The combination of osteoconductive scaffolds and osteoinductive growth factors is considered to have synergistic effects on bone regeneration. Materials and methods: In this study, core–shell poly(lactide-co-glycolide) (PLGA)/polycaprolactone (PCL)–BMP-2 (PP–B) fibrous scaffolds were prepared through coaxial electrospinning. Next, we fabricated 3D scaffolds based on PP–B fibers with thermally induced self-agglomeration (TISA) method and compared with conventional PLGA/PCL scaffolds in terms of scaffold morphology and BMP-2 release behaviors. Then, rat adipose-derived stem cells (rADSCs) were seeded on the scaffolds, and the effects on cell proliferation, cell morphology, and osteogenic differentiation of rADSCs were detected. Results: The results demonstrated that 3D scaffold incorporated with BMP-2 significantly increased proliferation and osteogenic differentiation of rADSCs, followed by PP–B group. Conclusion: Our findings indicate that scaffolds with 3D structure and osteoinductive growth factors have great potential in bone tissue engineering. Keywords: BMP-2, core–shell scaffolds, 3D fibrous scaffolds, osteogenic differentiationhttps://www.dovepress.com/thermally-induced-self-agglomeration-3d-scaffolds-with-bmp-2-loaded-co-peer-reviewed-article-IJNBMP-2core-shell scaffolds3D fibrous scaffoldsosteogenic differentiation |
| spellingShingle | Hu S Chen H Zhou X Chen G Hu K Cheng Y Wang L Zhang F Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells International Journal of Nanomedicine BMP-2 core-shell scaffolds 3D fibrous scaffolds osteogenic differentiation |
| title | Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells |
| title_full | Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells |
| title_fullStr | Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells |
| title_full_unstemmed | Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells |
| title_short | Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells |
| title_sort | thermally induced self agglomeration 3d scaffolds with bmp 2 loaded core ndash shell fibers for enhanced osteogenic differentiation of rat adipose derived stem cells |
| topic | BMP-2 core-shell scaffolds 3D fibrous scaffolds osteogenic differentiation |
| url | https://www.dovepress.com/thermally-induced-self-agglomeration-3d-scaffolds-with-bmp-2-loaded-co-peer-reviewed-article-IJN |
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