An Overview of Adenoid Microbiome Using 16S rRNA Gene Sequencing-Based Metagenomic Analysis
<i>Background and Objectives</i>: the upper respiratory tract harbors the highest bacterial density in the whole respiratory system. Adenoids, which are located in the <i>nasopharynx</i>, are a major site of bacterial colonies in the upper airways. Our goal was to use culture...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
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| Series: | Medicina |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1648-9144/58/7/920 |
| Summary: | <i>Background and Objectives</i>: the upper respiratory tract harbors the highest bacterial density in the whole respiratory system. Adenoids, which are located in the <i>nasopharynx</i>, are a major site of bacterial colonies in the upper airways. Our goal was to use culture-independent molecular techniques to identify the breadth of bacterial diversity in the adenoid vegetations of children suffering from chronic rhinosinusitis and obstructive sleep apnea. <i>Materials and methods</i>: in total, 21 adenoid samples were investigated using amplification and sequencing of the V3-V4 hypervariable region of the bacterial 16S rRNA gene. <i>Results</i>: among the most common bacterial species found were <i>Veillonella atypica</i>, <i>Fusobactrium nucelatum</i>, <i>Shaalia odontolytica</i>, and <i>Moraxella catarrhalis</i>. <i>Veillonella atypica</i> and <i>Fusbacterium</i><i>nucelatum</i> dominated the microbiome in all 21 samples, attributing to more than 60% of all detected genetic material. <i>Conclusions</i>: since both <i>Veillonella atypica</i> and <i>Fusobacterium nucleatum</i> are, predominantly, oral cavity and dental microorganisms, our findings may suggest oral microbiome migration deeper into the oropharynx and nasopharynx where these bacteria colonize adenoid vegetations. |
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| ISSN: | 1010-660X 1648-9144 |