Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in Mice
In the last decade, e-cigarette usage has increased, with an estimated 82 million e-cigarette users globally. This is, in part, due to the common opinion that they are “healthier” than tobacco cigarettes or simply “water vapour”. Third-hand e-vapour exposure is the chemical residue left behind from...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/11/9/749 |
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| author | Andrew E. Thorpe Chantal Donovan Richard Y. Kim Howard J. Vindin Razia Zakarya Hanna Miyai Yik L. Chan David van Reyk Hui Chen Brian G. Oliver |
| author_facet | Andrew E. Thorpe Chantal Donovan Richard Y. Kim Howard J. Vindin Razia Zakarya Hanna Miyai Yik L. Chan David van Reyk Hui Chen Brian G. Oliver |
| author_sort | Andrew E. Thorpe |
| collection | DOAJ |
| description | In the last decade, e-cigarette usage has increased, with an estimated 82 million e-cigarette users globally. This is, in part, due to the common opinion that they are “healthier” than tobacco cigarettes or simply “water vapour”. Third-hand e-vapour exposure is the chemical residue left behind from e-cigarette aerosols, which is of concern due to its invisible nature, especially among young children. However, there is limited information surrounding third-hand e-vapour exposure. This study aimed to investigate the pulmonary effects of sub-chronic third-hand e-vapour exposure in a murine model. BALB/c mice (4 weeks of age) were exposed to a towel containing nicotine free (0 mg) e-vapour, nicotine (18 mg) e-vapour, or no e-vapour (sham) and replaced daily for 4 weeks. At the endpoint, lung function was assessed, and bronchoalveolar lavage fluid and lungs were collected to measure inflammation and fibrosis. Mice exposed to third-hand e-vapour without nicotine had alveolar enlargement compared to sham exposed controls. Mice exposed to third-hand e-vapour with nicotine had reduced bronchial responsiveness to provocation, increased epithelial thickening in large airways, increased epithelial layers in small airways, alveolar enlargement, and increased small airway collagen deposition, compared to sham exposed controls. In conclusion, our study shows that third-hand e-vapour exposure, particularly in the presence of nicotine, negatively affects the lung health of mice and highlights the need for greater public awareness surrounding the dangers of third-hand exposure to e-cigarette vapour. |
| first_indexed | 2024-03-10T21:53:28Z |
| format | Article |
| id | doaj.art-43cc2bfe71a9445eb5994607ee13f488 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-10T21:53:28Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-43cc2bfe71a9445eb5994607ee13f4882023-11-19T13:14:37ZengMDPI AGToxics2305-63042023-09-0111974910.3390/toxics11090749Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in MiceAndrew E. Thorpe0Chantal Donovan1Richard Y. Kim2Howard J. Vindin3Razia Zakarya4Hanna Miyai5Yik L. Chan6David van Reyk7Hui Chen8Brian G. Oliver9School of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, AustraliaSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, AustraliaSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, AustraliaRespiratory Cellular and Molecular Biology, Woolcock Institute of Medical Research, Macquarie University, Glebe, NSW 2037, AustraliaSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, AustraliaSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, AustraliaSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, AustraliaSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, AustraliaSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, AustraliaSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, AustraliaIn the last decade, e-cigarette usage has increased, with an estimated 82 million e-cigarette users globally. This is, in part, due to the common opinion that they are “healthier” than tobacco cigarettes or simply “water vapour”. Third-hand e-vapour exposure is the chemical residue left behind from e-cigarette aerosols, which is of concern due to its invisible nature, especially among young children. However, there is limited information surrounding third-hand e-vapour exposure. This study aimed to investigate the pulmonary effects of sub-chronic third-hand e-vapour exposure in a murine model. BALB/c mice (4 weeks of age) were exposed to a towel containing nicotine free (0 mg) e-vapour, nicotine (18 mg) e-vapour, or no e-vapour (sham) and replaced daily for 4 weeks. At the endpoint, lung function was assessed, and bronchoalveolar lavage fluid and lungs were collected to measure inflammation and fibrosis. Mice exposed to third-hand e-vapour without nicotine had alveolar enlargement compared to sham exposed controls. Mice exposed to third-hand e-vapour with nicotine had reduced bronchial responsiveness to provocation, increased epithelial thickening in large airways, increased epithelial layers in small airways, alveolar enlargement, and increased small airway collagen deposition, compared to sham exposed controls. In conclusion, our study shows that third-hand e-vapour exposure, particularly in the presence of nicotine, negatively affects the lung health of mice and highlights the need for greater public awareness surrounding the dangers of third-hand exposure to e-cigarette vapour.https://www.mdpi.com/2305-6304/11/9/749e-cigarettesvapinge-vapingnicotinelung functionfibrosis |
| spellingShingle | Andrew E. Thorpe Chantal Donovan Richard Y. Kim Howard J. Vindin Razia Zakarya Hanna Miyai Yik L. Chan David van Reyk Hui Chen Brian G. Oliver Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in Mice Toxics e-cigarettes vaping e-vaping nicotine lung function fibrosis |
| title | Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in Mice |
| title_full | Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in Mice |
| title_fullStr | Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in Mice |
| title_full_unstemmed | Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in Mice |
| title_short | Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in Mice |
| title_sort | third hand exposure to e cigarette vapour induces pulmonary effects in mice |
| topic | e-cigarettes vaping e-vaping nicotine lung function fibrosis |
| url | https://www.mdpi.com/2305-6304/11/9/749 |
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