Changes in Methane Emission and Community Composition of Methane-Cycling Microorganisms Along an Elevation Gradient in the Dongting Lake Floodplain, China
Methane (CH<sub>4</sub>) emission and environmental controls of CH<sub>4</sub>-cycling microorganisms are unclear in inland floodplains. Here, we examined soil CH<sub>4</sub> emissions and the community composition of CH<sub>4</sub>-cycling microorgani...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-09-01
|
| Series: | Atmosphere |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4433/11/9/997 |
| _version_ | 1827706000088498176 |
|---|---|
| author | Yijie Ren Lianlian Zhu Zhengmiao Deng Yonghong Xie Chengyi Zhang Fengjin Xiao Xinsheng Chen Feng Li Yeai Zou Rong Sheng Xiaoyan Zhang Xian Chen |
| author_facet | Yijie Ren Lianlian Zhu Zhengmiao Deng Yonghong Xie Chengyi Zhang Fengjin Xiao Xinsheng Chen Feng Li Yeai Zou Rong Sheng Xiaoyan Zhang Xian Chen |
| author_sort | Yijie Ren |
| collection | DOAJ |
| description | Methane (CH<sub>4</sub>) emission and environmental controls of CH<sub>4</sub>-cycling microorganisms are unclear in inland floodplains. Here, we examined soil CH<sub>4</sub> emissions and the community composition of CH<sub>4</sub>-cycling microorganisms under three vegetation types—mudflat (MF, no vegetation cover), <i>Carex</i> meadow (CM, mainly <i>Carex brevicuspis</i>), and reed land (RL, mainly <i>Miscanthus sacchariflorus</i>)—from water-adjacent areas to higher-elevation land in the Dongting Lake floodplain, China. The results showed that CH<sub>4</sub> emission is the highest in CM, while significant absorption was observed in the RL site. The abundance ratio of methanogen/methanotroph was the highest in CM, intermediate in MF, and lowest in RL. The <i>Methanosarcinaceae</i> family represented the dominant methanogens in the three sampling sites (41.32–75.25%). The genus <i>Methylocystis</i> (60.85%, type II methanotrophs) was dominant in CM, while <i>Methylobacter</i> and <i>Methylosarcina</i> (type I methanotrophs) were the dominant genera in MF (51.00%) and RL (50.24%), respectively. Structural equation model analysis showed that methanogen and methanotroph abundance were affected by water table depth, soil water content, and pH indirectly through soil organic content, total nitrogen, microbial biomass carbon, and microbial biomass nitrogen. These results indicated that the Dongting Lake floodplain may change from a CH<sub>4</sub> source to a CH<sub>4</sub> sink with vegetation succession with an increase in elevation, and the methanogen/methanotroph ratio can be used as a proxy for CH<sub>4</sub> emission in wetland soils. The continuous increase in reed area combined with the decrease in <i>Carex</i> meadow may mitigate CH<sub>4</sub> emission and enhance the CH<sub>4</sub> sink function during the non-flood season in the Dongting Lake floodplain. |
| first_indexed | 2024-03-10T16:14:23Z |
| format | Article |
| id | doaj.art-43cd6db7caa344da9b6dba576fc0ecdf |
| institution | Directory Open Access Journal |
| issn | 2073-4433 |
| language | English |
| last_indexed | 2024-03-10T16:14:23Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Atmosphere |
| spelling | doaj.art-43cd6db7caa344da9b6dba576fc0ecdf2023-11-20T14:08:26ZengMDPI AGAtmosphere2073-44332020-09-0111999710.3390/atmos11090997Changes in Methane Emission and Community Composition of Methane-Cycling Microorganisms Along an Elevation Gradient in the Dongting Lake Floodplain, ChinaYijie Ren0Lianlian Zhu1Zhengmiao Deng2Yonghong Xie3Chengyi Zhang4Fengjin Xiao5Xinsheng Chen6Feng Li7Yeai Zou8Rong Sheng9Xiaoyan Zhang10Xian Chen11Key Laboratory of Agro-ecological Processes in Subtropical Region, The Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, The Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, The Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, The Chinese Academy of Sciences, Changsha 410125, ChinaNational Climate Center, China Meteorological Administration, Beijing 100081, ChinaNational Climate Center, China Meteorological Administration, Beijing 100081, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, The Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, The Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, The Chinese Academy of Sciences, Changsha 410125, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, The Chinese Academy of Sciences, Changsha 410125, ChinaSchool of Agriculture, Sun Yat-sen University, Guangzhou 510275, ChinaHunan Research Center of Agricultural Microorganism Engineering Technology, Hunan Institute of Microbiology, Changsha 410009, ChinaMethane (CH<sub>4</sub>) emission and environmental controls of CH<sub>4</sub>-cycling microorganisms are unclear in inland floodplains. Here, we examined soil CH<sub>4</sub> emissions and the community composition of CH<sub>4</sub>-cycling microorganisms under three vegetation types—mudflat (MF, no vegetation cover), <i>Carex</i> meadow (CM, mainly <i>Carex brevicuspis</i>), and reed land (RL, mainly <i>Miscanthus sacchariflorus</i>)—from water-adjacent areas to higher-elevation land in the Dongting Lake floodplain, China. The results showed that CH<sub>4</sub> emission is the highest in CM, while significant absorption was observed in the RL site. The abundance ratio of methanogen/methanotroph was the highest in CM, intermediate in MF, and lowest in RL. The <i>Methanosarcinaceae</i> family represented the dominant methanogens in the three sampling sites (41.32–75.25%). The genus <i>Methylocystis</i> (60.85%, type II methanotrophs) was dominant in CM, while <i>Methylobacter</i> and <i>Methylosarcina</i> (type I methanotrophs) were the dominant genera in MF (51.00%) and RL (50.24%), respectively. Structural equation model analysis showed that methanogen and methanotroph abundance were affected by water table depth, soil water content, and pH indirectly through soil organic content, total nitrogen, microbial biomass carbon, and microbial biomass nitrogen. These results indicated that the Dongting Lake floodplain may change from a CH<sub>4</sub> source to a CH<sub>4</sub> sink with vegetation succession with an increase in elevation, and the methanogen/methanotroph ratio can be used as a proxy for CH<sub>4</sub> emission in wetland soils. The continuous increase in reed area combined with the decrease in <i>Carex</i> meadow may mitigate CH<sub>4</sub> emission and enhance the CH<sub>4</sub> sink function during the non-flood season in the Dongting Lake floodplain.https://www.mdpi.com/2073-4433/11/9/997methanotrophmethanogenDongting Lake floodplainvegetation typemethane flux |
| spellingShingle | Yijie Ren Lianlian Zhu Zhengmiao Deng Yonghong Xie Chengyi Zhang Fengjin Xiao Xinsheng Chen Feng Li Yeai Zou Rong Sheng Xiaoyan Zhang Xian Chen Changes in Methane Emission and Community Composition of Methane-Cycling Microorganisms Along an Elevation Gradient in the Dongting Lake Floodplain, China Atmosphere methanotroph methanogen Dongting Lake floodplain vegetation type methane flux |
| title | Changes in Methane Emission and Community Composition of Methane-Cycling Microorganisms Along an Elevation Gradient in the Dongting Lake Floodplain, China |
| title_full | Changes in Methane Emission and Community Composition of Methane-Cycling Microorganisms Along an Elevation Gradient in the Dongting Lake Floodplain, China |
| title_fullStr | Changes in Methane Emission and Community Composition of Methane-Cycling Microorganisms Along an Elevation Gradient in the Dongting Lake Floodplain, China |
| title_full_unstemmed | Changes in Methane Emission and Community Composition of Methane-Cycling Microorganisms Along an Elevation Gradient in the Dongting Lake Floodplain, China |
| title_short | Changes in Methane Emission and Community Composition of Methane-Cycling Microorganisms Along an Elevation Gradient in the Dongting Lake Floodplain, China |
| title_sort | changes in methane emission and community composition of methane cycling microorganisms along an elevation gradient in the dongting lake floodplain china |
| topic | methanotroph methanogen Dongting Lake floodplain vegetation type methane flux |
| url | https://www.mdpi.com/2073-4433/11/9/997 |
| work_keys_str_mv | AT yijieren changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina AT lianlianzhu changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina AT zhengmiaodeng changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina AT yonghongxie changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina AT chengyizhang changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina AT fengjinxiao changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina AT xinshengchen changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina AT fengli changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina AT yeaizou changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina AT rongsheng changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina AT xiaoyanzhang changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina AT xianchen changesinmethaneemissionandcommunitycompositionofmethanecyclingmicroorganismsalonganelevationgradientinthedongtinglakefloodplainchina |