Multibow: digital spectral barcodes for cell tracing.
We introduce a multicolor labeling strategy (Multibow) for cell tracing experiments in developmental and regenerative processes. Building on Brainbow-based approaches that produce colors by differential expression levels of different fluorescent proteins, Multibow adds a layer of label diversity by...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4444339?pdf=render |
| _version_ | 1811281522469109760 |
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| author | Fengzhu Xiong Nikolaus D Obholzer Ramil R Noche Sean G Megason |
| author_facet | Fengzhu Xiong Nikolaus D Obholzer Ramil R Noche Sean G Megason |
| author_sort | Fengzhu Xiong |
| collection | DOAJ |
| description | We introduce a multicolor labeling strategy (Multibow) for cell tracing experiments in developmental and regenerative processes. Building on Brainbow-based approaches that produce colors by differential expression levels of different fluorescent proteins, Multibow adds a layer of label diversity by introducing a binary code in which reporters are initially OFF and then probabilistically ON or OFF following Cre recombination. We have developed a library of constructs that contains seven different colors and three different subcellular localizations. Combining constructs from this library in the presence of Cre generates cells labeled with multiple independently expressed colors based on if each construct is ON or OFF following recombination. These labels form a unique "barcode" that allows the tracking of the cell and its clonal progenies in addition to expression level differences of each color. We tested Multibow in zebrafish which validates its design concept and suggests its utility for cell tracing applications in development and regeneration. |
| first_indexed | 2024-04-13T01:35:05Z |
| format | Article |
| id | doaj.art-43cd875b13d845c29f3a1a598990fed5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T01:35:05Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-43cd875b13d845c29f3a1a598990fed52022-12-22T03:08:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012782210.1371/journal.pone.0127822Multibow: digital spectral barcodes for cell tracing.Fengzhu XiongNikolaus D ObholzerRamil R NocheSean G MegasonWe introduce a multicolor labeling strategy (Multibow) for cell tracing experiments in developmental and regenerative processes. Building on Brainbow-based approaches that produce colors by differential expression levels of different fluorescent proteins, Multibow adds a layer of label diversity by introducing a binary code in which reporters are initially OFF and then probabilistically ON or OFF following Cre recombination. We have developed a library of constructs that contains seven different colors and three different subcellular localizations. Combining constructs from this library in the presence of Cre generates cells labeled with multiple independently expressed colors based on if each construct is ON or OFF following recombination. These labels form a unique "barcode" that allows the tracking of the cell and its clonal progenies in addition to expression level differences of each color. We tested Multibow in zebrafish which validates its design concept and suggests its utility for cell tracing applications in development and regeneration.http://europepmc.org/articles/PMC4444339?pdf=render |
| spellingShingle | Fengzhu Xiong Nikolaus D Obholzer Ramil R Noche Sean G Megason Multibow: digital spectral barcodes for cell tracing. PLoS ONE |
| title | Multibow: digital spectral barcodes for cell tracing. |
| title_full | Multibow: digital spectral barcodes for cell tracing. |
| title_fullStr | Multibow: digital spectral barcodes for cell tracing. |
| title_full_unstemmed | Multibow: digital spectral barcodes for cell tracing. |
| title_short | Multibow: digital spectral barcodes for cell tracing. |
| title_sort | multibow digital spectral barcodes for cell tracing |
| url | http://europepmc.org/articles/PMC4444339?pdf=render |
| work_keys_str_mv | AT fengzhuxiong multibowdigitalspectralbarcodesforcelltracing AT nikolausdobholzer multibowdigitalspectralbarcodesforcelltracing AT ramilrnoche multibowdigitalspectralbarcodesforcelltracing AT seangmegason multibowdigitalspectralbarcodesforcelltracing |