Effect of CB1 Receptor Deficiency on Mitochondrial Quality Control Pathways in Gastrocnemius Muscle
This study aims to explore the complex role of cannabinoid type 1 receptor (CB1) signaling in the gastrocnemius muscle, assessing physiological processes in both CB1<sup>+/+</sup> and CB1<sup>−/−</sup> mice. The primary focus is to enhance our understanding of how CB1 contrib...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/13/2/116 |
| _version_ | 1797298814887919616 |
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| author | Rosalba Senese Giuseppe Petito Elena Silvestri Maria Ventriglia Nicola Mosca Nicoletta Potenza Aniello Russo Francesco Manfrevola Gilda Cobellis Teresa Chioccarelli Veronica Porreca Vincenza Grazia Mele Rosanna Chianese Pieter de Lange Giulia Ricci Federica Cioffi Antonia Lanni |
| author_facet | Rosalba Senese Giuseppe Petito Elena Silvestri Maria Ventriglia Nicola Mosca Nicoletta Potenza Aniello Russo Francesco Manfrevola Gilda Cobellis Teresa Chioccarelli Veronica Porreca Vincenza Grazia Mele Rosanna Chianese Pieter de Lange Giulia Ricci Federica Cioffi Antonia Lanni |
| author_sort | Rosalba Senese |
| collection | DOAJ |
| description | This study aims to explore the complex role of cannabinoid type 1 receptor (CB1) signaling in the gastrocnemius muscle, assessing physiological processes in both CB1<sup>+/+</sup> and CB1<sup>−/−</sup> mice. The primary focus is to enhance our understanding of how CB1 contributes to mitochondrial homeostasis. At the tissue level, CB1<sup>−/−</sup> mice exhibit a substantial miRNA-related alteration in muscle fiber composition, characterized by an enrichment of oxidative fibers. CB1 absence induces a significant increase in the oxidative capacity of muscle, supported by elevated in-gel activity of Complex I and Complex IV of the mitochondrial respiratory chain. The increased oxidative capacity is associated with elevated oxidative stress and impaired antioxidant defense systems. Analysis of mitochondrial biogenesis markers indicates an enhanced capacity for new mitochondria production in CB1<sup>−/−</sup> mice, possibly adapting to altered muscle fiber composition. Changes in mitochondrial dynamics, mitophagy response, and unfolded protein response (UPR) pathways reveal a dynamic interplay in response to CB1 absence. The interconnected mitochondrial network, influenced by increased fusion and mitochondrial UPR components, underlines the dual role of CB1 in regulating both protein quality control and the generation of new mitochondria. These findings deepen our comprehension of the CB1 impact on muscle physiology, oxidative stress, and MQC processes, highlighting cellular adaptability to CB1<sup>−/−</sup>. This study paves the way for further exploration of intricate signaling cascades and cross-talk between cellular compartments in the context of CB1 and mitochondrial homeostasis. |
| first_indexed | 2024-03-07T22:41:32Z |
| format | Article |
| id | doaj.art-43cd8a8545604357b204acebff3db205 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-07T22:41:32Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-43cd8a8545604357b204acebff3db2052024-02-23T15:08:13ZengMDPI AGBiology2079-77372024-02-0113211610.3390/biology13020116Effect of CB1 Receptor Deficiency on Mitochondrial Quality Control Pathways in Gastrocnemius MuscleRosalba Senese0Giuseppe Petito1Elena Silvestri2Maria Ventriglia3Nicola Mosca4Nicoletta Potenza5Aniello Russo6Francesco Manfrevola7Gilda Cobellis8Teresa Chioccarelli9Veronica Porreca10Vincenza Grazia Mele11Rosanna Chianese12Pieter de Lange13Giulia Ricci14Federica Cioffi15Antonia Lanni16Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Sciences and Technologies, University of Sannio, 82100 Benevento, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Sciences and Technologies, University of Sannio, 82100 Benevento, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyThis study aims to explore the complex role of cannabinoid type 1 receptor (CB1) signaling in the gastrocnemius muscle, assessing physiological processes in both CB1<sup>+/+</sup> and CB1<sup>−/−</sup> mice. The primary focus is to enhance our understanding of how CB1 contributes to mitochondrial homeostasis. At the tissue level, CB1<sup>−/−</sup> mice exhibit a substantial miRNA-related alteration in muscle fiber composition, characterized by an enrichment of oxidative fibers. CB1 absence induces a significant increase in the oxidative capacity of muscle, supported by elevated in-gel activity of Complex I and Complex IV of the mitochondrial respiratory chain. The increased oxidative capacity is associated with elevated oxidative stress and impaired antioxidant defense systems. Analysis of mitochondrial biogenesis markers indicates an enhanced capacity for new mitochondria production in CB1<sup>−/−</sup> mice, possibly adapting to altered muscle fiber composition. Changes in mitochondrial dynamics, mitophagy response, and unfolded protein response (UPR) pathways reveal a dynamic interplay in response to CB1 absence. The interconnected mitochondrial network, influenced by increased fusion and mitochondrial UPR components, underlines the dual role of CB1 in regulating both protein quality control and the generation of new mitochondria. These findings deepen our comprehension of the CB1 impact on muscle physiology, oxidative stress, and MQC processes, highlighting cellular adaptability to CB1<sup>−/−</sup>. This study paves the way for further exploration of intricate signaling cascades and cross-talk between cellular compartments in the context of CB1 and mitochondrial homeostasis.https://www.mdpi.com/2079-7737/13/2/116cannabinoid type 1 receptor (CB1)skeletal musclemitochondriamitochondrial quality control (MQC)miRNAmitochondrial unfolded protein response (UPRmt) |
| spellingShingle | Rosalba Senese Giuseppe Petito Elena Silvestri Maria Ventriglia Nicola Mosca Nicoletta Potenza Aniello Russo Francesco Manfrevola Gilda Cobellis Teresa Chioccarelli Veronica Porreca Vincenza Grazia Mele Rosanna Chianese Pieter de Lange Giulia Ricci Federica Cioffi Antonia Lanni Effect of CB1 Receptor Deficiency on Mitochondrial Quality Control Pathways in Gastrocnemius Muscle Biology cannabinoid type 1 receptor (CB1) skeletal muscle mitochondria mitochondrial quality control (MQC) miRNA mitochondrial unfolded protein response (UPRmt) |
| title | Effect of CB1 Receptor Deficiency on Mitochondrial Quality Control Pathways in Gastrocnemius Muscle |
| title_full | Effect of CB1 Receptor Deficiency on Mitochondrial Quality Control Pathways in Gastrocnemius Muscle |
| title_fullStr | Effect of CB1 Receptor Deficiency on Mitochondrial Quality Control Pathways in Gastrocnemius Muscle |
| title_full_unstemmed | Effect of CB1 Receptor Deficiency on Mitochondrial Quality Control Pathways in Gastrocnemius Muscle |
| title_short | Effect of CB1 Receptor Deficiency on Mitochondrial Quality Control Pathways in Gastrocnemius Muscle |
| title_sort | effect of cb1 receptor deficiency on mitochondrial quality control pathways in gastrocnemius muscle |
| topic | cannabinoid type 1 receptor (CB1) skeletal muscle mitochondria mitochondrial quality control (MQC) miRNA mitochondrial unfolded protein response (UPRmt) |
| url | https://www.mdpi.com/2079-7737/13/2/116 |
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