A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis
Objective: The objective of this prospective observational case–control study is to evaluate the prevalence of <i>Fusobacterium nucleatum</i> in the tissues of oral squamous cell carcinoma (OSCC). Reconnoitering the CCL20-related mechanism of carcinogenesis in <i>Fusobacterium nucl...
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MDPI AG
2021-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/11/1218 |
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| author | Devi Prasad Mandal Neeta Mohanty Paresh Kumar Behera Divya Gopinath Sasmita Panda Abdulaziz A. Al-Kheraif Darshan Devang Divakar Sukumaran Anil Swagatika Panda |
| author_facet | Devi Prasad Mandal Neeta Mohanty Paresh Kumar Behera Divya Gopinath Sasmita Panda Abdulaziz A. Al-Kheraif Darshan Devang Divakar Sukumaran Anil Swagatika Panda |
| author_sort | Devi Prasad Mandal |
| collection | DOAJ |
| description | Objective: The objective of this prospective observational case–control study is to evaluate the prevalence of <i>Fusobacterium nucleatum</i> in the tissues of oral squamous cell carcinoma (OSCC). Reconnoitering the CCL20-related mechanism of carcinogenesis in <i>Fusobacterium nucleatum</i>-positive OSCC is another objective. Methodology: Tissues from 50 OSCC patients and 30 healthy oral tissues were collected. The prevalence of <i>Fusobacterium nucleatum</i> was evaluated in both tumour and healthy tissue by polymerase chain reaction. The immunohistochemistry of OSCC tissues was conducted to evaluate the difference in the expression of CCL20 between <i>Fusobacterium nucleatum</i>-positive and -negative OSCC tissues. Results: <i>Fusobacterium nucleatum</i> was significantly (<i>p</i> < 0.001) prevalent in OSCC tissues (74%), compared to healthy tissues (26%). No association of <i>Fusobacterium nucleatum</i> or CCL20 immuno-expression with any clinical or histopathological features of OSCC was observed. While the intensity of CCL20 immuno-expression did not differ (<i>p</i> = 0.053), the CCL20-positive cell population was significantly different (<i>p</i> = 0.034) between <i>Fusobacterium nucleatum</i>-positive and -negative OSCC. Conclusion: <i>Fusobacterium nucleatum</i> is possibly prevalent in oral cancer tissues in the Indian population. By using immunohistochemistry, this is the first study to propose that the carcinogenesis in <i>Fusobacterium nucleatum</i>-positive OSCC may be CCL20-related. The findings enrich the knowledge of mechanisms involved in <i>Fusobacterium nucleatum</i>-mediated oral carcinogenesis. |
| first_indexed | 2024-03-10T05:20:36Z |
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| id | doaj.art-43cdc6676e73475eb69c637df19b79a6 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T05:20:36Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-43cdc6676e73475eb69c637df19b79a62023-11-23T00:04:36ZengMDPI AGLife2075-17292021-11-011111121810.3390/life11111218A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral CarcinogenesisDevi Prasad Mandal0Neeta Mohanty1Paresh Kumar Behera2Divya Gopinath3Sasmita Panda4Abdulaziz A. Al-Kheraif5Darshan Devang Divakar6Sukumaran Anil7Swagatika Panda8Institute of Dental Sciences, Siksha ’O’ Anusandhan Deemed to Be University, Bhubaneswar 751030, Odisha, IndiaDepartment of Oral Pathology and Microbiology, Institute of Dental Sciences, Siksha ’O’ Anusandhan Deemed to Be University, Bhubaneswar 751030, Odisha, IndiaHead and Neck Oncology, Acharya Harihar Regional Cancer Centre, Cuttack 753007, Odisha, IndiaClinical Oral Health Sciences Division, School of Dentistry, International Medical University, Kuala Lumpur 57000, MalaysiaDepartment of Pathology, Acharya Harihar Regional Cancer Centre, Cuttack 753007, Odisha, IndiaDental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi ArabiaDental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi ArabiaDepartment of Dentistry, Oral Health Institute, Hamad Medical Corporation, Doha P.O. Box 3050, QatarDepartment of Oral Pathology and Microbiology, Institute of Dental Sciences, Siksha ’O’ Anusandhan Deemed to Be University, Bhubaneswar 751030, Odisha, IndiaObjective: The objective of this prospective observational case–control study is to evaluate the prevalence of <i>Fusobacterium nucleatum</i> in the tissues of oral squamous cell carcinoma (OSCC). Reconnoitering the CCL20-related mechanism of carcinogenesis in <i>Fusobacterium nucleatum</i>-positive OSCC is another objective. Methodology: Tissues from 50 OSCC patients and 30 healthy oral tissues were collected. The prevalence of <i>Fusobacterium nucleatum</i> was evaluated in both tumour and healthy tissue by polymerase chain reaction. The immunohistochemistry of OSCC tissues was conducted to evaluate the difference in the expression of CCL20 between <i>Fusobacterium nucleatum</i>-positive and -negative OSCC tissues. Results: <i>Fusobacterium nucleatum</i> was significantly (<i>p</i> < 0.001) prevalent in OSCC tissues (74%), compared to healthy tissues (26%). No association of <i>Fusobacterium nucleatum</i> or CCL20 immuno-expression with any clinical or histopathological features of OSCC was observed. While the intensity of CCL20 immuno-expression did not differ (<i>p</i> = 0.053), the CCL20-positive cell population was significantly different (<i>p</i> = 0.034) between <i>Fusobacterium nucleatum</i>-positive and -negative OSCC. Conclusion: <i>Fusobacterium nucleatum</i> is possibly prevalent in oral cancer tissues in the Indian population. By using immunohistochemistry, this is the first study to propose that the carcinogenesis in <i>Fusobacterium nucleatum</i>-positive OSCC may be CCL20-related. The findings enrich the knowledge of mechanisms involved in <i>Fusobacterium nucleatum</i>-mediated oral carcinogenesis.https://www.mdpi.com/2075-1729/11/11/1218oral squamous cell carcinoma<i>Fusobacterium nucleatum</i>CCL20 |
| spellingShingle | Devi Prasad Mandal Neeta Mohanty Paresh Kumar Behera Divya Gopinath Sasmita Panda Abdulaziz A. Al-Kheraif Darshan Devang Divakar Sukumaran Anil Swagatika Panda A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis Life oral squamous cell carcinoma <i>Fusobacterium nucleatum</i> CCL20 |
| title | A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis |
| title_full | A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis |
| title_fullStr | A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis |
| title_full_unstemmed | A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis |
| title_short | A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis |
| title_sort | plausible proposition of ccl20 related mechanism in i fusobacterium nucleatum i associated oral carcinogenesis |
| topic | oral squamous cell carcinoma <i>Fusobacterium nucleatum</i> CCL20 |
| url | https://www.mdpi.com/2075-1729/11/11/1218 |
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