<span style="font-variant: small-caps">l</span>-Lactate Dehydrogenase B Chain Associated with Milk Protein Content in Dairy Cows
This study aimed to explore genes associated with milk protein content in dairy cows and their relationships with <span style="font-variant: small-caps;">l</span>-leucine. Ten primiparous Holstein cows (93.8 ± 11.56 milking days) fed the same diet were divided into two...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/9/7/442 |
| _version_ | 1818421965357056000 |
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| author | Tao Wang Seung Woo Jeon U Suk Jung Min Jeong Kim Hong Gu Lee |
| author_facet | Tao Wang Seung Woo Jeon U Suk Jung Min Jeong Kim Hong Gu Lee |
| author_sort | Tao Wang |
| collection | DOAJ |
| description | This study aimed to explore genes associated with milk protein content in dairy cows and their relationships with <span style="font-variant: small-caps;">l</span>-leucine. Ten primiparous Holstein cows (93.8 ± 11.56 milking days) fed the same diet were divided into two groups depending on their milk protein contents (group High, 3.34 ± 0.10%; and group Low, 2.86 ± 0.05%). Milk epithelial cells (MECs) were isolated from the collected morning milk and differentially expressed proteins in MECs were explored by two-dimensional gel electrophoresis (2-DE). Then, the mRNA expression of these proteins was detected by real time PCR in MAC-T cells incubated with three different media named positive control (PC), negative control (NC), and <span style="font-variant: small-caps;">l</span>-leucine depletion (NO-leu). Results showed that ten proteins were differentially expressed in MECs from cows in group High. They included seven down-regulated ones (heat shock protein beta-1 (HSPB1), 78 kDa glucose-regulated protein (GRP-78), <span style="font-variant: small-caps;">l</span>-lactate dehydrogenase B chain (LDH-B), malate dehydrogenase, cytoplasmic (MDH1), annexin I (ANXA1), cytokeratin-7 (CK-7), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)), and three up-regulated ones (prohibitin (PHB), beta casein (CSN2), and alpha S1 casein (CSN1S1)). When <span style="font-variant: small-caps;">l</span>-leucine was depleted from the medium, not only proteins content was lowered (<i>p</i> < 0.05), but also the LDH-B mRNA expression was decreased in MAC-T cells (<i>p</i> < 0.05). In conclusion, LDH-B is negatively associated with the milk protein content of dairy cows and has a positive association with <span style="font-variant: small-caps;">l</span>-leucine. |
| first_indexed | 2024-12-14T13:18:44Z |
| format | Article |
| id | doaj.art-43d1a07c670c42148152f8e6a6430d21 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-12-14T13:18:44Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-43d1a07c670c42148152f8e6a6430d212022-12-21T22:59:58ZengMDPI AGAnimals2076-26152019-07-019744210.3390/ani9070442ani9070442<span style="font-variant: small-caps">l</span>-Lactate Dehydrogenase B Chain Associated with Milk Protein Content in Dairy CowsTao Wang0Seung Woo Jeon1U Suk Jung2Min Jeong Kim3Hong Gu Lee4Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, KoreaDepartment of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, KoreaDepartment of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, KoreaDepartment of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, KoreaDepartment of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, KoreaThis study aimed to explore genes associated with milk protein content in dairy cows and their relationships with <span style="font-variant: small-caps;">l</span>-leucine. Ten primiparous Holstein cows (93.8 ± 11.56 milking days) fed the same diet were divided into two groups depending on their milk protein contents (group High, 3.34 ± 0.10%; and group Low, 2.86 ± 0.05%). Milk epithelial cells (MECs) were isolated from the collected morning milk and differentially expressed proteins in MECs were explored by two-dimensional gel electrophoresis (2-DE). Then, the mRNA expression of these proteins was detected by real time PCR in MAC-T cells incubated with three different media named positive control (PC), negative control (NC), and <span style="font-variant: small-caps;">l</span>-leucine depletion (NO-leu). Results showed that ten proteins were differentially expressed in MECs from cows in group High. They included seven down-regulated ones (heat shock protein beta-1 (HSPB1), 78 kDa glucose-regulated protein (GRP-78), <span style="font-variant: small-caps;">l</span>-lactate dehydrogenase B chain (LDH-B), malate dehydrogenase, cytoplasmic (MDH1), annexin I (ANXA1), cytokeratin-7 (CK-7), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)), and three up-regulated ones (prohibitin (PHB), beta casein (CSN2), and alpha S1 casein (CSN1S1)). When <span style="font-variant: small-caps;">l</span>-leucine was depleted from the medium, not only proteins content was lowered (<i>p</i> < 0.05), but also the LDH-B mRNA expression was decreased in MAC-T cells (<i>p</i> < 0.05). In conclusion, LDH-B is negatively associated with the milk protein content of dairy cows and has a positive association with <span style="font-variant: small-caps;">l</span>-leucine.https://www.mdpi.com/2076-2615/9/7/442milk epithelial cells<span style="font-variant: small-caps">l</span>-leucineMAC-T cellsmilk protein content<span style="font-variant: small-caps">l</span>-lactate dehydrogenase B chain |
| spellingShingle | Tao Wang Seung Woo Jeon U Suk Jung Min Jeong Kim Hong Gu Lee <span style="font-variant: small-caps">l</span>-Lactate Dehydrogenase B Chain Associated with Milk Protein Content in Dairy Cows Animals milk epithelial cells <span style="font-variant: small-caps">l</span>-leucine MAC-T cells milk protein content <span style="font-variant: small-caps">l</span>-lactate dehydrogenase B chain |
| title | <span style="font-variant: small-caps">l</span>-Lactate Dehydrogenase B Chain Associated with Milk Protein Content in Dairy Cows |
| title_full | <span style="font-variant: small-caps">l</span>-Lactate Dehydrogenase B Chain Associated with Milk Protein Content in Dairy Cows |
| title_fullStr | <span style="font-variant: small-caps">l</span>-Lactate Dehydrogenase B Chain Associated with Milk Protein Content in Dairy Cows |
| title_full_unstemmed | <span style="font-variant: small-caps">l</span>-Lactate Dehydrogenase B Chain Associated with Milk Protein Content in Dairy Cows |
| title_short | <span style="font-variant: small-caps">l</span>-Lactate Dehydrogenase B Chain Associated with Milk Protein Content in Dairy Cows |
| title_sort | span style font variant small caps l span lactate dehydrogenase b chain associated with milk protein content in dairy cows |
| topic | milk epithelial cells <span style="font-variant: small-caps">l</span>-leucine MAC-T cells milk protein content <span style="font-variant: small-caps">l</span>-lactate dehydrogenase B chain |
| url | https://www.mdpi.com/2076-2615/9/7/442 |
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