Effect of Sleep Restriction on Cardiometabolic and Haemoinflammatory Parameters in Adult Male Wistar Rats
Background and Objective: While insufficient sleep remains an under-recognized public health issue across the globe, there is paucity and heterogeneity of data regarding its cardiometabolic and haemoinflammatory implications. We, therefore, aimed to evaluate the impact of chronic sleep restriction o...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2021-11-01
|
| Series: | Journal of Sleep Sciences |
| Subjects: | |
| Online Access: | https://jss.tums.ac.ir/index.php/jss/article/view/174 |
| _version_ | 1828139938997075968 |
|---|---|
| author | Mahdi Dissi Gambo Ahmed Ibrahim A Salisu Yusuf Tanko Mohammed Aliyu |
| author_facet | Mahdi Dissi Gambo Ahmed Ibrahim A Salisu Yusuf Tanko Mohammed Aliyu |
| author_sort | Mahdi Dissi Gambo |
| collection | DOAJ |
| description | Background and Objective: While insufficient sleep remains an under-recognized public health issue across the globe, there is paucity and heterogeneity of data regarding its cardiometabolic and haemoinflammatory implications. We, therefore, aimed to evaluate the impact of chronic sleep restriction on cardiometabolic and haemoinflammatory parame-ters in rats.
Materials and Methods: 16 male Wistar rats (aged 8-10 weeks) were randomly assigned into equal control or sleep restriction groups. Gentle handling was used to induce sleep restriction for six weeks. Fasting weight and blood sugar were obtained and lipids were analyzed using their respective Randox kits. Malondialdehyde (MDA) levels and catalase (CAT) and superoxide dismutase (SOD) activities were assayed. Full blood count and CD4+ T cell count were deter-mined using automated analyzer. Data were analyzed using Student’s t-test, with level of significance set at P ≤ 0.05, via SPSS software.
Results: Chronic sleep restriction caused significant initial weight loss, increase in feed consumption, and percentage in-crease in fasting blood sugar (FBS) (32% vs. 15%). We also noted the triglyceride-glucose (TyG) index of sleep-restricted rats to be significantly higher (6.22) than that of controls (5.62). In addition, a significant reduction in monocyte count, monocyte-lymphocyte ratio (MLR), and absolute CD4+ cell count among the sleep-restricted rats was observed.
Conclusion: Our findings have provided objective evidence that, over the course of 6 weeks, 5 hours of sleep re-striction had caused body weight gain, hyperglycaemia, insulin resistance, and impairment in immunoinflammatory status; hence, it could be a risk factor for developing cardiometabolic syndrome and immune-related disorders. |
| first_indexed | 2024-04-11T19:00:35Z |
| format | Article |
| id | doaj.art-43d27a3276a84d429673600306c3e439 |
| institution | Directory Open Access Journal |
| issn | 2476-2938 2476-2946 |
| language | English |
| last_indexed | 2024-04-11T19:00:35Z |
| publishDate | 2021-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Sleep Sciences |
| spelling | doaj.art-43d27a3276a84d429673600306c3e4392022-12-22T04:08:03ZengTehran University of Medical SciencesJournal of Sleep Sciences2476-29382476-29462021-11-015410.18502/jss.v5i4.7804Effect of Sleep Restriction on Cardiometabolic and Haemoinflammatory Parameters in Adult Male Wistar RatsMahdi Dissi Gambo0Ahmed Ibrahim A Salisu1Yusuf Tanko2Mohammed Aliyu3Department of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University, Kano, NigeriaDepartment of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University, Kano, NigeriaDepartment of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria, NigeriaDepartment of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria, NigeriaBackground and Objective: While insufficient sleep remains an under-recognized public health issue across the globe, there is paucity and heterogeneity of data regarding its cardiometabolic and haemoinflammatory implications. We, therefore, aimed to evaluate the impact of chronic sleep restriction on cardiometabolic and haemoinflammatory parame-ters in rats. Materials and Methods: 16 male Wistar rats (aged 8-10 weeks) were randomly assigned into equal control or sleep restriction groups. Gentle handling was used to induce sleep restriction for six weeks. Fasting weight and blood sugar were obtained and lipids were analyzed using their respective Randox kits. Malondialdehyde (MDA) levels and catalase (CAT) and superoxide dismutase (SOD) activities were assayed. Full blood count and CD4+ T cell count were deter-mined using automated analyzer. Data were analyzed using Student’s t-test, with level of significance set at P ≤ 0.05, via SPSS software. Results: Chronic sleep restriction caused significant initial weight loss, increase in feed consumption, and percentage in-crease in fasting blood sugar (FBS) (32% vs. 15%). We also noted the triglyceride-glucose (TyG) index of sleep-restricted rats to be significantly higher (6.22) than that of controls (5.62). In addition, a significant reduction in monocyte count, monocyte-lymphocyte ratio (MLR), and absolute CD4+ cell count among the sleep-restricted rats was observed. Conclusion: Our findings have provided objective evidence that, over the course of 6 weeks, 5 hours of sleep re-striction had caused body weight gain, hyperglycaemia, insulin resistance, and impairment in immunoinflammatory status; hence, it could be a risk factor for developing cardiometabolic syndrome and immune-related disorders.https://jss.tums.ac.ir/index.php/jss/article/view/174Sleep deprivationMetabolic syndromeBlood cell countCD4 positive cells |
| spellingShingle | Mahdi Dissi Gambo Ahmed Ibrahim A Salisu Yusuf Tanko Mohammed Aliyu Effect of Sleep Restriction on Cardiometabolic and Haemoinflammatory Parameters in Adult Male Wistar Rats Journal of Sleep Sciences Sleep deprivation Metabolic syndrome Blood cell count CD4 positive cells |
| title | Effect of Sleep Restriction on Cardiometabolic and Haemoinflammatory Parameters in Adult Male Wistar Rats |
| title_full | Effect of Sleep Restriction on Cardiometabolic and Haemoinflammatory Parameters in Adult Male Wistar Rats |
| title_fullStr | Effect of Sleep Restriction on Cardiometabolic and Haemoinflammatory Parameters in Adult Male Wistar Rats |
| title_full_unstemmed | Effect of Sleep Restriction on Cardiometabolic and Haemoinflammatory Parameters in Adult Male Wistar Rats |
| title_short | Effect of Sleep Restriction on Cardiometabolic and Haemoinflammatory Parameters in Adult Male Wistar Rats |
| title_sort | effect of sleep restriction on cardiometabolic and haemoinflammatory parameters in adult male wistar rats |
| topic | Sleep deprivation Metabolic syndrome Blood cell count CD4 positive cells |
| url | https://jss.tums.ac.ir/index.php/jss/article/view/174 |
| work_keys_str_mv | AT mahdidissigambo effectofsleeprestrictiononcardiometabolicandhaemoinflammatoryparametersinadultmalewistarrats AT ahmedibrahimasalisu effectofsleeprestrictiononcardiometabolicandhaemoinflammatoryparametersinadultmalewistarrats AT yusuftanko effectofsleeprestrictiononcardiometabolicandhaemoinflammatoryparametersinadultmalewistarrats AT mohammedaliyu effectofsleeprestrictiononcardiometabolicandhaemoinflammatoryparametersinadultmalewistarrats |